Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of histone deacetylase gene oshdt701 in prolonging plant seed life

A technology of deacetylase and histone, applied in the fields of application, plant products, genetic engineering, etc., to achieve the effect of prolonging the life of plant seeds and great application prospects

Active Publication Date: 2022-04-08
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the role of histone deacetylase OsHDT701 or its encoding gene in regulating plant seed lifespan.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of histone deacetylase gene oshdt701 in prolonging plant seed life
  • Application of histone deacetylase gene oshdt701 in prolonging plant seed life
  • Application of histone deacetylase gene oshdt701 in prolonging plant seed life

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Obtaining of overexpressed transgenic rice with histone deacetylase gene OsHDT701

[0029] In order to construct the OsHDT701 overexpression vector, the cDNA fragment of OsHDT701 (accession number: AK072845) was first amplified, and then the fragment was inserted into the vector pUbiO1301 (plasmid map as shown in figure 1 shown), the vector was modified based on pCAMIBA1301, and a maize ubiquitin promoter was added. The constructed vector was transformed into Agrobacterium strain EHA105, and then into the embryogenic callus of mature seeds of rice "Zhonghua 11". Specifically include the following steps:

[0030] 1. Construction of histone deacetylase gene OsHDT701 overexpression vector

[0031] Design specific primers according to the full-length sequence of the histone deacetylase gene 0sHDT701 known by NCBI, and at the same time add BamH I restriction sites to the 5' ends of the primers, and the amplification primers are:

[0032] oxHDT701F: 5'-CG GGATC...

Embodiment 2

[0055] Example 2 Obtaining of Histone Deacetylase Gene OsHDT701 RNAi Transgenic Rice

[0056] The OsHDT701 RNAi transgenic rice material is based on the cDNA sequence of OsHDT701 and its protein conserved domain, using the Zhonghua 11 seedling cDNA as a template to amplify the RNAi target fragment of about 300bp in the OsHDT701 gene, construct it on the interference vector, and use Agrobacterium Rice RNAi transgenic lines were obtained by bacterial dipping technique, and RT-PCR analysis showed that the expression of OsHDT701 gene in the transgenic lines was lower than that in the wild type. Specifically include the following steps:

[0057] 1. Construction of OsHDT701 RNAi vector

[0058] Selecting an appropriate gene segment is the key to successfully constructing an RNA interference vector. The purpose of constructing the vector is to specifically and effectively minimize the expression level of the target gene. Put the cDNA sequence of the target gene on NCBI for comparis...

Embodiment 3

[0084] Example 3 Vitality detection of OsHDT701 overexpression / RNAi transgenic rice

[0085] The determination of seed life is measured according to the classical method, that is, the germination rate after artificial aging treatment (Chen2012, Bentsink et al., 2000; Tesnier et al., 2002; Oge et al., 2008; Rajjou and Debeaujon, 2008). The OsHDT701 overexpression transgenic rice obtained in Example 1 and the OsHDT701 RNAi transgenic rice obtained in Example 2 were respectively subjected to the following experiments:

[0086] 1. Artificial aging treatment

[0087] According to the method of Liu Jun (2000, Journal of Experimental Botany) and Chen (2012, Journal of Experimental Botany), with slight modifications, the artificial aging was completed in a sealed desiccator. Put the seeds in a large centrifuge tube with the cover removed and pretreat them at 15°C and 85% relative humidity (R.H) for 3 days, then transfer them to a well-balanced desiccator at 42°C and 85% R.H for 3-5 d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of histone deacetylase gene OsHDT701 in prolonging the life span of plant seeds. The present invention respectively constructs the overexpression vector of OsHDT701 and the RNAi silencing expression vector and transforms rice to obtain transgenic plants overexpressing OsHDT701 and knocking down expressing OsHDT701, and the results show that the transgenic rice seeds knocking down expressing OsHDT701 have longer Seed longevity and stronger ability to resist artificial aging; while rice seeds overexpressing OsHDT701 showed shorter seed life or weaker ability to resist artificial aging than wild-type seeds, indicating that OsHDT701 negatively regulates rice seed life, OsHDT701 Genes can be used to improve crop seed longevity.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, and more specifically relates to the application of histone deacetylase gene OsHDT701 in prolonging the lifespan of plant seeds. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world. More than half of the world's population takes it as a staple food. Rice production is a major event related to the national economy and people's livelihood, and is closely related to my country's food security. As the most basic means of production, seeds are a necessary condition for the development of agricultural production. Seed vigor and longevity play a decisive role in plant reproduction, crop yield, germplasm conservation and biodiversity. Because seed production is difficult to predict accurately, seeds often need to be stored. With the prolongation of storage time, most of the seeds begin to deteriorate, seriously affecting agricultura...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/80C12N15/82A01H5/10A01H6/46
CPCC12N9/80C12N15/8218C12N15/8267C12Y305/01098
Inventor 刘军贾俊婷刘勋成段俊赵金会
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI