Lactobacillus delbrueckii and soybean milk beverage obtained by fermenting lactobacillus delbrueckii
A Lactobacillus delbrueckii and soybean technology, applied in the field of microorganisms, can solve problems such as undiscovered, and achieve the effects of removing beany smell, improving antioxidant capacity, and increasing soybean isoflavone aglycone content
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Embodiment 1
[0034] The isolation and identification of embodiment 1 bacterial strain
[0035] 1. Screening of bacterial strains
[0036] Physalis water, the substrate for strain isolation, was taken from the Physalis water used in the traditional production process of Guilin fermented bean curd. The samples were packed in food-grade PET bottles, brought back to the laboratory under low temperature storage conditions, and stored at 4 °C. Physalis water was diluted with sterile water, spread on MRS medium containing 2% calcium carbonate, and cultured anaerobically in a constant temperature incubator at 37°C for 48 hours.
[0037] Select the suspected lactic acid bacteria colonies that produce calcium-dissolving circles for pure culture, and the number is MT772183. After repeating the purification three times, a single colony was picked for Gram staining, and it was observed that the cells were Gram-positive, long rod-shaped, and most of them grew in pairs, but also appeared singly. The co...
Embodiment 2
[0052] The mensuration of embodiment 2 bacterial strain β-glucosidase activity
[0053] The specific operations for determining the β-glucosidase in the fermentation broth and whole cells of Lactobacillus delbrueckii MT772183 are as follows:
[0054] Sample pretreatment: Lactobacillus delbrueckii MT772183 was inoculated in MRS liquid medium, cultured at 37°C for 24 hours, 5 mL of fermentation broth was taken in a 10 mL centrifuge tube, centrifuged at 8000 r / min for 20 minutes, and the precipitate and supernatant were collected. The bacterial cell pellet was washed twice with pH 5.0 potassium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, centrifuged to remove the supernatant, and the precipitate was dissolved in 5 mL of pH 5.0 potassium dihydrogen phosphate-disodium hydrogen phosphate buffer solution to make a bacterial suspension solution to adjust the OD of the bacterial suspension 600 value, so that the density of the bacterial strain is close to 10 9 C...
Embodiment 3
[0065] Determination of antioxidant capacity of embodiment 3 bacterial strains
[0066] 1. Preparation of cell-free extract
[0067] Lactobacillus delbrueckii MT772183 was inoculated in MRS liquid culture medium, cultured at 37°C for 24 hours, collected by centrifugation, washed 3 times with PBS buffer of pH 7.4, and then resuspended in the same amount of buffer, adjusting the number of bacteria at 10 10 About CFU / ml, the cells were ultrasonically disrupted in an ice bath, 4°C, 12000g, and centrifuged for 30min. The supernatant was collected to obtain a cell-free extract.
[0068] 2. The ability to scavenge hydroxyl radicals
[0069] Add 2.5mmol / L O-phenanthroline, 1mL each of pH7.4 PBS buffer and distilled water to the test tube, mix thoroughly and add 1mL 2.5mmol / L ferrous sulfate and 1mL 0.1% H 2 o 2 , 37°C water bath for 1 hour, measure the absorbance at 536nm and record it as A1; replace 1mL H with 1mL distilled water 2 o 2 OD 536 The value is A0; the OD of 1 mL o...
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