Method for preparing peanut meal
A technology of peanut meal and enzymolysis, which is applied in the fields of animal feed and bioengineering, can solve the problems of reduced disease resistance of fish, adverse effects, and liver cell damage in growing and fattening pigs, so as to improve comprehensive antioxidant properties and reduce arginine. The effect of improving acid content, overall protein quality
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[0043] (1) Preparation of peanut meal enzymatic hydrolyzate
[0044] Peanut meal (the peanut meal is completely crushed and passed through a 40-mesh sieve) is mixed with water at a ratio of 1:5 (w / w), and 0.1% cellulase (purchased from Baiyin Sanuo Biotechnology Co., Ltd.) and phytic acid are added respectively Enzyme (provided by Wuhan Xinhuayang Biological Co., Ltd.), enzymolysis at 50°C for 1h, inactivated in boiling water bath for 10min, supplemented with distilled water to 250mL, and then acid protease (provided by Weifang Kangdien Biotechnology Co., Ltd.) for a second After enzymatic hydrolysis and enzyme inactivation, adjust the pH to 5.5.
[0045] Optimization of the secondary enzymatic hydrolysis conditions, changing the conditions of acidic protease enzymatic hydrolysis of peanut meal, and investigating the effect of the degree of protein hydrolysis of peanut meal, specifically changing the substrate enzyme activity, substrate mass fraction, enzymatic hydrolysis temp...
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