Method for efficiently recovering protein from excess sludge

A technology for excess sludge and protein, applied in the preparation method of peptides, plastic recycling, sludge treatment, etc., can solve the problems of unsuitable protein recovery, complex protein composition, low protein content, etc., to promote sludge dispersion, avoid Effects of overconsumption, rapid biodegradability

Active Publication Date: 2021-04-09
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with distiller's grain waste, potato processing wastewater, and seafood processing wastewater, the protein content is low and the protein composition is complex
In addition, the composition of WAS is complex, and various influencing substances such as heavy metals and antibiotics will be produced during the hydrolysis process.
Therefore, existing protein recovery methods are not suitable for the recovery of proteins in WAS

Method used

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  • Method for efficiently recovering protein from excess sludge

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]This embodiment is a method of efficiently recovering proteins from the remaining sludge (WAS), which includes the following steps:

[0033]The first step: sludge removed from the sewage treatment plant sludge, after 24 hours at 4 ° C, first discharge the supernatant with a straw, and filtered the sludge with a piece of aperture of 1 mm. The impurities in sludge were sieved, and finally the treated sludge was stored in a 4 ° C in an environment for subsequent experiments, and the SS of WAS was adjusted at around 10,000 mg / L, and the remaining sludge was obtained. This processing process is mainly to sieve the impurities in WAS and prepare for the back pre-processing.

[0034]Step 2: Take the residual sludge WAS in an anaerobic reactor having a capacitive volume of 500 ml, adjusting the pH of WAS 10.0 ± 0.2, then add 0.3 g / gs of APG at a temperature of 35 ± 1 ° C at a temperature of 35 ± 1 ° C. After the reaction, the reaction bottle was performed after 2-5 min, and the reaction ...

Embodiment 2

[0040]In the present embodiment, the present embodiment is in that, the amount of the APG in the present embodiment is added according to 0.1, 0.2, 0.4, 0.5 g / gs, and the recovery rate of the protein in this example is less than 90. %, But above 80%.

[0041]Under the conditions of the APG investment of 0.3 g / gss and pH of 10.0, the hydrolysis effect of WAS is preferably, the released protein content is high, which significantly improves the crack effect of sludge. Compared with the unprocessed sludge, the amount of SCOD of the APG was 0.0 ± 0.2, and the amount of SCOD of WAS was increased from 200.2 mg / L to 4500.0mg / L, protein dissolution amount. From 17.1 mg / L to 1009.7 mg / l, the amount of polysaccharide increased from 10.7 mg / L to 663.9 mg / L, and the SCOD content was increased by 22.5 times, and the protein content was increased by 59.0 times, and the polysaccharide was increased by 61.87 times.

Embodiment 3

[0043]In the present embodiment, the present embodiment is in that the pre-processed time is 0, 4, 8, 12, 24, 32, 48h in the present embodiment. During the WAS hydrolysis process, the highest concentration of SCOD, protein and polysaccharide increases with an increase in APG investment, but changes over time. When the reaction time was 2 h, the highest concentration of SCOD, protein and polysaccharide was 3250.1, 798.9, 478.1 mg / L, and the blank group SCOD, protein and polysaccharide were only 200.2, 17.2, 10.9 mg / L. The amount of protein is reduced in the amount of protein in the reaction time than 8 h, which is not conducive to efficient recovery of proteins.

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Abstract

The invention relates to a method for efficiently recovering protein from excess sludge. The method comprises the following steps: adding excess sludge WAS into a reaction flask, adding APG according to the addition amount of 0.1-0.5 g/gSS, and regulating the initial pH value to 4.0-11.0; after the reaction bottle is subjected to oxygen expelling and nitrogen charging for 2-5 min, sealing the reaction bottle and carrying out constant-temperature shaking culture for WAS hydrolysis; centrifuging pretreated WAS to completely precipitate the residual sludge WAS, taking the supernatant, then adding carrageenan into the supernate, enabling the mass ratio of protein obtained by WAS hydrolysis to the carrageenan to be (25-1):1, regulating the pH value to 1.0-3.0, and oscillating at the temperature of 30-37 DEG C for 15 minutes to fully mix the carrageenan with the protein and the acid; and carrying out centrifugal treatment on the mixed solution to obtain a lower precipitate, namely the recycled protein. According to the process, after hydrolysis, the technology of combining a complex coacervation method and an acid-base precipitation method is adopted, and protein is recycled to the maximum extent through the combined action of the complex coacervation method and the acid-base precipitation method.

Description

Technical field[0001]The present invention belongs to the technical field of solid waste treatment, and in particular, a method of efficiently recovering proteins from the remaining sludge.Background technique[0002]It is estimated that by 2020, my country's sludge production will reach 80 million tons, and the problem of Waste ActivatedSludge, Was has caused a wide range of attention. The conventional processing method not only causes waste of resources, but also increases the environmental burden. Therefore, high-efficiency recovery of resources in sludge, and improving sludge treatment efficiency have broad application prospects. Sludge contains a large number of recycled resources, such as proteins, heavy metals, and the like. And the protein and cationic substances in sludge are also affected by the surface properties, sedimentation and dehydration properties of the sludge. Therefore, the recovery of proteins in WAS can not only meet the reduction target of WAS, but also the res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/32C07K1/14C02F11/127C02F11/02C02F11/00
CPCC07K1/32C07K1/145C02F11/02C02F11/00C02F11/127Y02W30/62
Inventor 李相昆谢红伟刘改革杨瑛俊刘富荣孙钰洁李佳泽
Owner HEBEI UNIV OF TECH
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