CO<2>/N<2> switch type fluorescent liquid-liquid phase separation nano-droplet and preparation method thereof

A liquid phase separation and droplet technology, which can be applied to preparations for in vivo experiments, medical preparations with inactive ingredients, and pharmaceutical formulas, etc. It can solve the problems of biomedical application limitations and achieve good temperature stability.

Active Publication Date: 2021-04-20
GUANGDONG PHARMA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The condensed phase nano-droplets produced by the liquid-liquid phase separation currently constructed often need to be additionally loaded with dyes in order to achieve the purpose of fluorescence tracking. In biomedicine application is limited

Method used

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  • CO&lt;2&gt;/N&lt;2&gt; switch type fluorescent liquid-liquid phase separation nano-droplet and preparation method thereof
  • CO&lt;2&gt;/N&lt;2&gt; switch type fluorescent liquid-liquid phase separation nano-droplet and preparation method thereof
  • CO&lt;2&gt;/N&lt;2&gt; switch type fluorescent liquid-liquid phase separation nano-droplet and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for preparing liquid-liquid phase separation nano-droplets constructed of nonionic surfactants and carbon quantum dots, comprising the following steps:

[0044] 1) The concentration of immobilized S-CQDs was 2mg / mL, and 10mM, 15mM, 20mM, 25mM, 28mM, 29mM, 30mM, 31mM, 32mM, 33mM, 34mM, 35mM, 36mM, 37mM, 38mM, 39mM, 40mM, 41mM, 42mM, 43mM, 44mM, 45mM, 46mM, 47mM, 48mM, 49mM, 50mM PKOO solution, measure the absorbance at ultraviolet 600nm, draw the phase behavior of the system as PKOO changes with the concentration (see attached figure 1 );

[0045] 2) After the mixed solution with a concentration of 34mM PKOO and 2mg / mL S-CQDs was placed for 24 hours, the solution changed from turbid to clear, and the supernatant was discarded by centrifugation at 10,000rpm for 30min to obtain a viscous solid in the condensed phase of liquid-liquid phase separation;

[0046] 3) Dissolve 5 mg of the viscous solid in the condensed phase with 1 mL of deionized water to prepare a na...

Embodiment 2

[0049] A method for preparing liquid-liquid phase separation nano-droplets constructed of nonionic surfactants and carbon quantum dots, comprising the following steps:

[0050] 1) Take a mixed solution with a concentration of 40mM PKOO and 2mg / mL Se-CQDs and place it for 24h, the solution turns from turbid to clear, centrifuge at 10000rpm for 30min and discard the supernatant to obtain a viscous solid in the condensed phase;

[0051] 3) Take the viscous solid of the condensed phase and dissolve it with deionized water to prepare a 5 mg / mL nano-droplet solution, and store it at room temperature for later use.

Embodiment 3

[0053] Prepare solutions containing different concentrations of Nile Red and 5mg / mL nano-droplets, the specific steps are as follows:

[0054] 1) Take 5 test tubes (number: 1-5), add 20 μL of 1 mg / mL Nile Red methanol solution to the test tube, and dry in a vacuum drying phase at 25°C for two hours to remove the methanol solvent;

[0055] 2) According to the method of Example 1, the coacervated phase viscous solid was prepared, and the nano-droplet solution of 5 mg / mL was prepared, and different amounts of 5 mg / mL of the nano-droplet solution were added to No. 1-4 test tubes (with 5 mg / mL). / mL nano-droplet solution is regarded as solvent) to prepare Nile Red solutions containing different concentrations (the concentrations of Nile Red No. Add pure water to No. test tube to prepare 40μg / mL Nile red aqueous solution; keep away from light, incubate for 18h, measure fluorescence and observe under fluorescence microscope (see appendix Figure 5 , 6 )

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Abstract

The invention belongs to the field of fluorescent carrier nanotechnologies, and particularly relates to a CO<2> / N<2> switch type fluorescent liquid-liquid phase separation nano-droplet and a preparation method thereof. Preparation raw materials in the invention include oleamide propyl dimethyl tertiary amine and carbon quantum dots; the fluorescent nano-droplet is constructed by electrostatic interaction force, hydrogen bonds and the like between a surfactant PKOO and the carbon quantum dots; the construction method is simple; and the constructed nano-droplet has the characteristics of pH responsiveness, CO<2> / N<2> responsiveness, fluorescence characteristic and the like.

Description

technical field [0001] The invention belongs to the field of fluorescent carrier nanotechnology, in particular to a CO 2 / N 2 A switch-type fluorescent liquid-liquid phase separation nano-droplet and a preparation method thereof. Background technique [0002] Liquid-liquid phase separation is the process of spontaneously generating liquid-liquid phase separation between two polyelectrolytes through weak interaction forces such as electrostatic interactions and hydrogen bonds, during which spherical nano-droplets can be formed. The nanodroplets can be controlled by the chemical nature, ratio, equilibration time, pH value and ionic strength of the polyelectrolytes. Due to its own characteristics, liquid-liquid phase separation has received extensive attention in the study of primitive cells. It can enrich and isolate a variety of molecules, including biological macromolecules such as proteins, enzymes, DNA, etc., and can be used in condensed phases. The catalytic activity o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K47/18A61K47/04A61K9/107A61K48/00
Inventor 刘意伍春闲郑茵刘勇严志红冯冰洁
Owner GUANGDONG PHARMA UNIV
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