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Sodium alginate-hydroxyapatite hybrid nanoparticle double-layer membrane and preparation method thereof

A technology of hybrid nanoparticles and hydroxyapatite, applied in the field of biomedical materials, can solve the problems of insufficient cell adhesion, lack of biocompatibility, low wettability, etc., to avoid insufficient adhesion, high Effect of biocompatibility and high wettability

Pending Publication Date: 2021-04-20
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies in the above-mentioned prior art, the present invention provides a method for preparing a sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film, the purpose of which is to solve the existing problems of polycaprolactone-based double-layer films in the prior art. was developed, however, such membranes have low wettability and lack of biocompatibility, leading to technical problems of insufficient adhesion of cells to the pore walls during the transport of nutrients in biomaterials

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  • Sodium alginate-hydroxyapatite hybrid nanoparticle double-layer membrane and preparation method thereof

Examples

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Effect test

Embodiment 1

[0025] The preparation method of the sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film provided in this example, the specific technical scheme is as follows:

[0026] A method for preparing a sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film, comprising the steps of:

[0027] S1, 6g of calcium chloride, 10g of sodium phosphate and 75g of sodium nitrite were sequentially added to 875mL of a solution containing 1g of cetyltrimethylammonium bromide, followed by hydrothermal treatment to obtain a mixed solution.

[0028] S2, the mixed solution in step S1 was filtered through a filter paper with a pore size of 2.5 μm, and then calcined at 400° C. for 5 hours to obtain hydroxyapatite hybrid nanoparticles (Han particles).

[0029] S3. Stir the sodium alginate aqueous solution with a mass concentration of 2g / mL at 40°C and 350rmp for 3 hours to obtain an aqueous sodium alginate solution. Add the aqueous sodium alginate solution to a petri dish wit...

Embodiment 2

[0034] The preparation method of the sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film provided in this example, the specific technical scheme is as follows:

[0035] A method for preparing a sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film, comprising the steps of:

[0036] S1, add 2.4g of calcium chloride, 4g of sodium phosphate and 30g of sodium nitrite to 350mL solution containing 0.4g of cetyltrimethylammonium bromide in sequence, and perform hydrothermal treatment to obtain a mixed solution.

[0037] S2, the mixed solution in step S1 was filtered through a filter paper with a pore size of 2.5 μm, and then calcined at 400° C. for 3 hours to obtain hydroxyapatite hybrid nanoparticles (Han particles).

[0038] S3, Stir the sodium alginate aqueous solution with a mass concentration of 3g / mL at 50°C and 350rmp for 5 hours to obtain an aqueous sodium alginate solution, add the aqueous sodium alginate solution to a petri dish with a diamet...

Embodiment 3

[0044] The preparation method of the sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film provided in this example, the specific technical scheme is as follows:

[0045] A method for preparing a sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film, comprising the steps of:

[0046] S1, add 2.75g of calcium chloride, 5.5g of sodium phosphate and 41.25g of sodium nitrite to 500mL solution containing 0.55g of cetyltrimethylammonium bromide (concentration: 3mM) successively, and perform hydrothermal treatment to obtain a mixed solution .

[0047] S2, the mixed solution in step S1 was filtered through a filter paper with a pore size of 2.5 μm, and then calcined at 450° C. for 2 hours to obtain hydroxyapatite hybrid nanoparticles (Han particles).

[0048] S3, Stir the sodium alginate aqueous solution with a mass concentration of 1g / mL at 45°C and 300rmp for 2 hours to obtain an aqueous sodium alginate solution, add the aqueous sodium alginate solutio...

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Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to a sodium alginate- hydroxyapatite hybrid nanoparticle double-layer membrane and a preparation method thereof. The method comprises steps as follows: sequentially adding calcium salt, sodium phosphate and sodium nitrite into the hexadecyl trimethyl ammonium bromide solution, and then filtering and forging the solution to obtain hydroxyapatite hybrid nanoparticles; then mixing the hydroxyapatite hybrid nanoparticles with calcium chloride to obtain a cross-linking solution; and finally, stirring and drying the cross-linking solution and an ALG membrane obtained by drying the sodium alginate solution to obtain the double-layer membrane. The outer side of the sodium alginate- hydroxyapatite hybrid nanoparticle double-layer membrane can limit growth of harmful soft connective tissue and support angiogenesis and wound stabilization, and the inner side of the sodium alginate-hydroxyapatite hybrid nanoparticle double-layer membrane can protect hemagglutination below and promote bone tissue regeneration, so that the sodium alginate-hydroxyapatite hybrid nanoparticle double-layer membrane needs to have high wettability, high biocompatibility and biodegradablity, and has relatively strong biological adhesion, so that insufficient adhesion of cells on pore walls is avoided.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film and a preparation method thereof. Background technique [0002] The periosteum is an asymmetric membrane that covers most of the bones in our body. Specifically, it is a dense, irregular connective tissue structure whose outer layer contains fibroblasts and microvessels and whose inner layer mainly contains osteoprogenitor cells. Since the periosteum plays an important role in the remodeling and metabolism of bone tissue, designing new biomaterials that mimic its structure is an attractive strategy for regenerative applications. [0003] Guided bone regeneration is a surgical approach widely used in the oral and maxillofacial region that utilizes a barrier membrane to guide bone growth while physically preventing the migration of non-osteogenic cells. The significance of this membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/52A61L27/58
Inventor 孔海磊许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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