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Paenibacillus ehime hd producing antimicrobial peptides and its application

A technology of bacillus and antimicrobial peptides, applied in the fields of application, antibacterial drugs, antifungal agents, etc., can solve the problem of the lack of reports on the antibacterial properties of Paenibacillus Ehime, general fungal inhibitory effect, and no mention of metabolites, etc. problem, to achieve excellent antifungal activity, good antibacterial effect, simple and easy-to-operate fermentation method

Active Publication Date: 2022-04-01
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A variety of extracellular antibacterial substances secreted by Paenibacillus have inhibitory activity against a variety of pathogenic bacteria, but there are not many reports on the antibacterial properties of Paenibacillus Ehime at home and abroad
In 2009, the team of Professor Lu Chengping of Nanjing Agricultural University reported a strain of Paenibacillus ehime NJ2008 with antibacterial properties, but there was no description of its antifungal properties, nor its metabolites; the Chinese invention patent with the announcement number CN110257269A The application discloses a bacteriocin-producing Paenibacillus ehime NPUST-1. The bacteriocin protein produced by the strain has an inhibitory effect on bacteria, but the inhibitory effect on fungi is general

Method used

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  • Paenibacillus ehime hd producing antimicrobial peptides and its application
  • Paenibacillus ehime hd producing antimicrobial peptides and its application
  • Paenibacillus ehime hd producing antimicrobial peptides and its application

Examples

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Effect test

Embodiment 1

[0033] Example 1 Screening and Identification of Paenibacillus Ehime HD Producing Antimicrobial Peptides

[0034] (1) Isolation and screening of bacterial strains

[0035] More than 40 soil samples were collected from Hebei, Jiangsu and other places. On the nutrient agar medium, the plate antibacterial method was used, Staphylococcus aureus and Penicillium chrysogenum were used as indicator bacteria, and the inhibition zone was used as the basis for screening. Preliminary screening of bacterial strains; after screening, 5 bacterial strains capable of inhibiting the growth of bacteria and fungal indicator bacteria were obtained, respectively numbered as MH, XM, YM, HD and GL; Record the diameter of the inhibition zone to Staphylococcus aureus and Penicillium chrysogenum, the results are shown in Table 1:

[0036] Screening of strains in Table 1

[0037] Strain number Diameter of inhibition zone against Staphylococcus aureus (mm) Diameter of inhibition zone again...

Embodiment 2-7

[0050] The fermentation method of the Paenibacillus ehime HD of embodiment 2-7 producing antimicrobial peptide

[0051] Embodiments 2-7 are respectively a fermentation method of Paenibacillus ehime producing antimicrobial peptides, specifically: after weighing the carbon source, nitrogen source, inorganic salt, and distilled water according to Table 3, mixing, adjusting the pH value, 121 After sterilizing at ℃ for 25 minutes, the improved fermentation medium was prepared; Paenibacillus ehime HD was inoculated into the corresponding improved fermentation medium, and fermented for 18-32 hours at a temperature of 28-32°C and a rotational speed of 220-240r / min ; Their difference only lies in the difference of raw material, consumption, initial pH value and process parameter, specifically see Table 3 for details:

[0052] Table 3 List of fermentation method parameters of Paenibacillus ehime HD producing antimicrobial peptides

[0053]

[0054] Wherein, the fermentation time is ...

Embodiment 8

[0055] Example 8 Effect Identification of the Fermentation Method of Paenibacillus Ehime HD Producing Antimicrobial Peptides

[0056] The fermentation method (after optimization) and the traditional fermentation method (before optimization) of Example 2 were respectively adopted to utilize Paenibacillus ehime HD to carry out long-term fermentation to produce antimicrobial peptides, and the fermentation time 0-60h was recorded every 10h respectively before adopting optimization. / The antimicrobial peptide yield of the post-fermentation method, the results are as follows Figure 4 shown;

[0057] Depend on Figure 4 It can be seen that compared with the traditional fermentation method before optimization, the production of antibacterial peptides by the optimized fermentation method of Example 2 of the present invention is significantly higher at 15-35h of fermentation, and the activity reaches the highest value at 32h of fermentation, compared with that before no optimization ...

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Abstract

The invention belongs to the technical field of fermentation engineering, and discloses an antimicrobial peptide-producing Paenibacillus ehime HD and its application. The strain was deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on August 7, 2020, and the preservation address is It is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.20505. The Paenibacillus ehime HD producing antimicrobial peptides provided by the present invention has fast growth, good adaptability and low fermentation cost, and the antimicrobial peptides produced by the fermentation method of the present invention have good stability and wide antibacterial spectrum, and can inhibit various bacteria and fungi , especially exhibits excellent antifungal activity, can effectively inhibit the activity of yeast and filamentous fungi; and this strain can also be used to produce biological preservatives and antibacterial agents, and the obtained products are used for food preservation and antibiotic-free breeding of livestock and poultry. field of prevention and control.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to Paenibacillus ehime, in particular to Paenibacillus ehime HD producing antimicrobial peptides and an application thereof. Background technique [0002] Microorganisms such as bacteria and fungi have caused various problems such as food pollution, plant diseases, and human infections, which have brought great harm to people's life and production. Difficulties; traditional synthetic antibacterial drugs have drug residues and side effects, which can no longer meet the demand. Therefore, no matter in the food industry, livestock and poultry breeding industry, or the pharmaceutical industry and plant control fields, there is an urgent need to develop safe and efficient antibacterial agents. [0003] Antimicrobial peptides, as the natural defense molecules of biological organisms, have a broad prospect of becoming safe and efficient antibacterial agents. At present, stud...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00A23K20/147A23L3/3526A23B7/154A61K38/16A61P31/04A61P31/10C12R1/01
Inventor 王志新贾英民宁亚维鲁雷震宏丹刘洋封成玲贾紫伟
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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