Xylooligosaccharide prepared from corn straw pulp cores and method thereof
A technology of corn stalks and xylo-oligosaccharides, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve the problems of cumbersome production process of xylo-oligosaccharides, and achieve the convenience of industrial production and simple operation process , the effect of improving the efficiency of enzymatic hydrolysis
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Embodiment 1
[0031] Dry, mold-free corn stalks were selected for dehulling and peeling to obtain corn stalk pith, which was crushed, washed, and dried in an oven at 45°C to constant weight for use. Take 5g of dried corn stalk core powder, add 75mL of deionized water, add solid heteropoly acid-phosphotungstic acid with a mass of 0.01g~0.1g and mix evenly for pretreatment. The reaction temperature is 75°C and the reaction time is 3h , down to room temperature after the reaction. Use 3 times the volume of 95% ethanol, let it stand overnight, filter under reduced pressure, and collect the filtrate; carry out enzymatic hydrolysis on the collected filtrate, add 500IU / g xylanase, and the enzymatic hydrolysis time is 6h, to obtain the enzymatic hydrolysis solution; The enzymolysis solution is treated with enzyme inactivation, and the enzymolysis solution is incubated at 100°C for 10 minutes to inactivate the enzyme, and then centrifuged in a high-speed centrifuge at 4000rpm for 15 minutes to remov...
Embodiment 2
[0035] Dry, mold-free corn stalks were selected for dehulling and peeling to obtain corn stalk pith, which was crushed, washed, and dried in an oven at 45°C to constant weight for use. Take 5g of dried corn stalk core powder, add 75mL of deionized water, add solid heteropoly acid-phosphotungstic acid with a mass of 0.08g and mix evenly for pretreatment. The reaction temperature is 55℃~95℃, and the reaction time is 3h , down to room temperature after the reaction. Use 3 times the volume of 95% ethanol, let it stand overnight, filter under reduced pressure, and collect the filtrate; carry out enzymatic hydrolysis on the collected filtrate, add 500IU / g xylanase, and the enzymatic hydrolysis time is 6h, to obtain the enzymatic hydrolysis solution; The enzymolysis solution is treated with enzyme inactivation, and the enzymolysis solution is incubated at 100°C for 10 minutes to inactivate the enzyme, and then centrifuged in a high-speed centrifuge at 4000rpm for 15 minutes to remove...
Embodiment 3
[0039] Dry, mold-free corn stalks were selected for dehulling and peeling to obtain corn stalk pith, which was crushed, washed, and dried in an oven at 45°C to constant weight for use. Take 5g of dried corn stalk core powder, add 75mL of deionized water, add solid heteropoly acid-phosphotungstic acid with a mass of 0.08g and mix evenly for pretreatment. The reaction temperature is 75°C, and the reaction time is 1-4h. Cool down to room temperature after the reaction. Use 3 times the volume of 95% ethanol, let it stand overnight, filter under reduced pressure, and collect the filtrate; carry out enzymatic hydrolysis on the collected filtrate, add 500IU / g xylanase, and the enzymatic hydrolysis time is 6h, to obtain the enzymatic hydrolysis solution; The enzymolysis solution is treated with enzyme inactivation, and the enzymolysis solution is incubated at 100°C for 10 minutes to inactivate the enzyme, and then centrifuged in a high-speed centrifuge at 4000rpm for 15 minutes to rem...
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