Preparation method of branch type walking machine aptamer electrochemical sensor for adenosine triphosphate detection

Abstract

The invention relates to a preparation method of a branch type walking machine aptamer electrochemical sensor for adenosine triphosphate detection, which comprises the following steps: preparing an acanthosphere-shaped bimetallic oxide / functionalized carbon nano composite material by adopting a hydrothermal synthesis method, and constructing a branch-type walking machine by self-designing complementary pairing of bases, constructing a branch walking machine / acanthosphere bimetallic oxide / functionalized carbon nano composite material / film gold electrode through covalent bonding, and reflecting the content of a target object adenosine triphosphate by signal change generated by T-DNA marked by signal molecules, so that the signal-reduced electrochemical aptamer sensor for detecting adenosine triphosphate is obtained. Compared with other electrochemical sensors for detecting the content of adenosine triphosphate, the prepared branch type walking machine aptamer electrochemical sensor has the advantages of high sensitivity, good repeatability and high accuracy.

Description

Technical field[0001]The present invention relates to a method for preparing a branch type walking machine for three-phospologic acid detection, and more particularly to a branch type walking machine / a borne bimetallic oxide / functionalized carbon nanocomposite / gold electrode method.Background technique[0002]Tripphosphate adenosine (ATP) is used as the main energy source of cells, involved in various biological processes, including cellular functions, muscle contraction, neuronal transmission, platelet function, membrane transport and vascular expansion, known as all organisms "molecular energy currency organisms". A key role in regulating cell metabolism and biochemical pathways in cell physiology. It has also been used as an indicator of cell viability and cellular injury, which plays a crucial biochemical reaction in regulating cell metabolism. Therefore, intracellular ATP concentration is closely related to the number of live cells. As a good biomarker, ATP is directly sens...

AI Technical Summary

Problems solved by technology

Traditional ATP detection methods are mainly based on high performance liquid chromatography, mass spectrometry and bioluminescence, however, these methods are complex and technically demanding

Nowadays, various novel analytical methods, including chemiluminescence, surface-enhanced Raman scattering, fluorescence, etc., have been widely explored to determine the amount of ATP. Although these methods can detect ATP with high sensitivity, the above methods are time-consuming and expensive in equipment. And the shortcomings of inconvenient on-site operation