Preparation method of branch type walking machine aptamer electrochemical sensor for adenosine triphosphate detection

An adenosine triphosphate and aptamer electrochemical technology is applied in the field of preparation of branch-type walking machine aptamer electrochemical sensors, which can solve the problems of high technical requirements, expensive equipment, complicated methods, etc., and achieves good repeatability, high accuracy and high sensitivity. Effect

Active Publication Date: 2021-05-07
HENAN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

Traditional ATP detection methods are mainly based on high performance liquid chromatography, mass spectrometry and bioluminescence, however, these methods are complex and technically demanding
Nowadays, various novel analytical methods, including chemiluminescence, surface-enhanced Raman scattering, fluorescence, etc., have been widely explored to determine the amount of ATP. Although these methods can detect ATP with high sensitivity, the above methods are time-consuming and expensive in equipment. And the shortcomings of inconvenient on-site operation

Method used

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Comparison scheme
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Embodiment 1

[0022]Specific steps are as follows:

[0023](1) Preparation of CO-LA Oxide / PEI-RGO Composites: Preparation of carbon nanomaterials by hydrothermal synthesis, 30 mg of carbon nanomaterials, dissolved in 20 ml of water, and tested 60 min. Then, a magnetic stirrer was slowly added to 2 ml of 3% amino functionalized solution at 450 rpm, and the mixed mixed solution of stirring was heated at 135 ° C for 3 h, and the obtained product was centrifuged at 8500 rpm (6 min) and water. 3 times was washed, dried at 60 ° C to obtain a functionalized carbon nanomaterial. 110 mg CoCl2· 4h2O, 180 mg LaCl3· 7h2O and 180 mg of urea dissolved in 20 mL mixed solution (H2O and ethanol, volume ratio 1: 1), and then prepared a mixed solution was added to 20 ml of 1.5 mg / ml PEI-RGO suspension, and the sonication was tested for 40 minutes at room temperature. The resulting solution was transferred into a 100 mL high pressure reactor and heated at 90 ° C in an electrothermal static drying tank for 16 h. Aft...

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Abstract

The invention relates to a preparation method of a branch type walking machine aptamer electrochemical sensor for adenosine triphosphate detection, which comprises the following steps: preparing an acanthosphere-shaped bimetallic oxide/functionalized carbon nano composite material by adopting a hydrothermal synthesis method, and constructing a branch-type walking machine by self-designing complementary pairing of bases, constructing a branch walking machine/acanthosphere bimetallic oxide/functionalized carbon nano composite material/film gold electrode through covalent bonding, and reflecting the content of a target object adenosine triphosphate by signal change generated by T-DNA marked by signal molecules, so that the signal-reduced electrochemical aptamer sensor for detecting adenosine triphosphate is obtained. Compared with other electrochemical sensors for detecting the content of adenosine triphosphate, the prepared branch type walking machine aptamer electrochemical sensor has the advantages of high sensitivity, good repeatability and high accuracy.

Description

Technical field[0001]The present invention relates to a method for preparing a branch type walking machine for three-phospologic acid detection, and more particularly to a branch type walking machine / a borne bimetallic oxide / functionalized carbon nanocomposite / gold electrode method.Background technique[0002]Tripphosphate adenosine (ATP) is used as the main energy source of cells, involved in various biological processes, including cellular functions, muscle contraction, neuronal transmission, platelet function, membrane transport and vascular expansion, known as all organisms "molecular energy currency organisms". A key role in regulating cell metabolism and biochemical pathways in cell physiology. It has also been used as an indicator of cell viability and cellular injury, which plays a crucial biochemical reaction in regulating cell metabolism. Therefore, intracellular ATP concentration is closely related to the number of live cells. As a good biomarker, ATP is directly sens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 何保山郑瑞娜谢玲玲曹晓雨白春启卫敏金华丽任文洁索志光吴立根王涛
Owner HENAN UNIVERSITY OF TECHNOLOGY
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