Inactivated virus preservation solution and preparation method thereof

Abstract

The invention relates to an inactivated virus preservation solution and a preparation method thereof. The inactivated virus preservation solution comprises guanidinium isothiocyanate, trihydroxymethyl aminomethane hydrochloride, tetrasodium iminodisuccinate, an anionic surfactant, a cationic surfactant and dithiothreitol. According to the inactivated virus preservation solution provided by the invention, not only the integrity of viral nucleic acid can be effectively guaranteed , but also the components for inactivating viruses are added, so that the inactivation performance of the viruses is guaranteed, a collected biological sample can be applied to nucleic acid detection, the risk of virus transmission infection is reduced, and subsequent nucleic acid extraction and amplification detection are not influenced.

Description

technical field [0001] The invention relates to an inactivated virus preservation solution and a preparation method thereof, belonging to the technical field of in vitro diagnosis. Background technique [0002] Viruses are non-cellular life forms that must parasitize and proliferate in living cells. They have a simple structure and are generally composed of nucleic acid (DNA or RNA) and a protein shell that wraps the nucleic acid. Most viruses are pathogenic microorganisms that can cause disease in the host and can spread in the organism to be infectious. At present, in vitro diagnosis can be carried out by collecting samples such as throat swabs, nasal swabs, and blood from patients to detect whether they are infected with a certain virus. Common detection methods are antigen antibody detection or nucleic acid detection on patient samples, but the virus generally leaves the host. Cells will be inactivated quickly, the integrity of the virus will be destroyed, the virus she...

AI Technical Summary

Problems solved by technology

At present, in vitro diagnosis can be carried out by collecting samples such as throat swabs, nasal swabs, and blood from patients to detect whether they are infected with a certain virus. Common detection methods are antigen antibody detection or nucleic acid detection on patient samples, but the virus generally leaves the host. Cells will be inactivated quickly, the integrity of the virus will be destroyed, the virus shell will disintegrate, and the nucleic acid will be degraded. Therefore, in order to ensure the accuracy of in vitro diagnosis, the transport and preservation of virus samples require specific equipment or preservation solutions.

[0003] At present, the main virus preservation solutions on the market are generally physiological saline, PBS buffer or UTM transport medium. of biological samples are at risk of secondary transmission of infection

[0004] At present, most inactivated virus preservation solutions on sale are inactivated with high-concentration guanidine salts. Although high-concentration guanidine salts have a better effect on virus inactivation, high-concentration guanidine salts are prone to crystallization at low temperatures (for example, samples collected at transportation in low-temperature areas in the north in winter), the inactivation effect of the virus may be reduced after precipitation, and the risk of infection transmission during transportation is increased; Residues of guanidinium salt in nucleic acid, which will inhibit nucleic acid amplification, lead to missed detection and increase the proportion of false negative samples

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