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Method for detecting 5-hydroxymethylcytosine by constructing photoelectrochemical sensor based on perovskite and black zirconium dioxide

A hydroxymethylcytosine, photoelectrochemical technology, applied in the field of photoelectrochemical analysis, can solve the problems of insufficient attention, high cost, too time-consuming, etc., and achieve the effect of improving the hole transfer rate, low cost, and easy operation.

Active Publication Date: 2021-05-28
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] 5hmC was first discovered in phage in 1952, but it did not attract enough attention at that time
However, the above methods either require specialized operators or are too expensive or time-consuming, so it is crucial to develop simple, fast, low-cost alternatives with high sensitivity and high selectivity
However, there is no perovskite-based Bi 4 TaO 8 A report on the detection of 5hmC by photoelectrochemical analysis of Cl and black zirconia

Method used

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  • Method for detecting 5-hydroxymethylcytosine by constructing photoelectrochemical sensor based on perovskite and black zirconium dioxide
  • Method for detecting 5-hydroxymethylcytosine by constructing photoelectrochemical sensor based on perovskite and black zirconium dioxide
  • Method for detecting 5-hydroxymethylcytosine by constructing photoelectrochemical sensor based on perovskite and black zirconium dioxide

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] (2) Black ZrO 2 Preparation: 0.5-2g ZrO 2 Grinding and mixing with 0.01-1g of sodium borohydride, calcining at 300-700°C for 0.5-3h in a nitrogen atmosphere, washing the obtained black solid powder with deionized water, and drying in vacuum. Finally, the product is ground and collected for later use.

[0054] (3) Aminated perovskite Bi 4 TaO 8 Cl-NH 2 Preparation of the dispersion: weigh 1-20 mg of aminated perovskite Bi 4 TaO 8 Cl-NH 2 , added to 3-10mL deionized water, and ultrasonically dispersed for 1-3 hours.

[0055] (4) Black ZrO 2 Preparation of dispersion: weigh 1-20mg black ZrO 2 , added to 1-10mL deionized water, and ultrasonically dispersed for 1-3 hours.

[0056] (5) ITO electrode pretreatment: cut the ITO conductive glass into 5×1cm 2 For small pieces, ultrasonically clean them with acetone for 30-60 minutes, and then ultrasonically clean them with NaOH (0.01-1M molar concentration) ethanol / water (volume ratio of ethanol to water: 1:1 to 1:3) mix...

Embodiment 1

[0076] Example 1: Aminated perovskite Bi 4 TaO 8 Cl-NH 2 preparation of

[0077] Weigh 1.7g Bi 2 o 3 ,0.5g Ta 2 o 5 , 0.6g BiOCl, 1.4g NaCl and 1.8g KCl powders were ground and mixed, and calcined at 700°C for 5 hours in an air atmosphere, and the calcined solid was taken out, and after grinding, it was centrifuged and washed with deionized water at 10,000rpm to collect the precipitate, and stored at 60°C Vacuum drying yields yellow perovskite Bi 4 TaO 8 Cl. Bi 4 TaO 8 Cl powder was ultrasonically dispersed in a mixed solution of ethanol and water (ethanol:water ratio 1:1), 3mL APTES solution was added under constant stirring, and heated to 70°C for reflux reaction for 7h. After cooling to room temperature, the precipitate was collected by centrifugation, and then the precipitate was sequentially washed with absolute ethanol and water at a speed of 10000 rpm, and the final product was vacuum-dried at 60°C and collected by grinding.

Embodiment 2

[0078] Example 2: Black ZrO 2 preparation of

[0079] White 1g ZrO 2 Grinding and mixing with 0.5g of sodium borohydride for 30min, calcining at 650°C for 3h under a nitrogen atmosphere to obtain a black solid powder, washing with deionized water, and finally the product was vacuum-dried and collected by grinding.

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Abstract

The invention discloses a method for detecting 5-hydroxymethylcytosine deoxyribonucleotide (5hmC) by constructing a photoelectrochemical sensor based on perovskite and black zirconium dioxide. The photoelectrochemical sensor comprises an electrode, and perovskite, thiohydracrylic acid, 5hmC and black ZrO2 which are sequentially modified on the electrode. Firstly, a photoelectrochemical biosensor with perovskite as a substrate is constructed, good photoelectric activity and stability of perovskite Bi4TaO8Cl, catalysis of a specific reaction of hydroxymethyl and sulfydryl on 5hmC by M.HhaI methyltransferase and specific recognition of black ZrO2 on a phosphate group are utilized, photogenerated electron transfer is promoted through a heterojunction formed by black ZrO2 and Bi4TaO8Cl, and a photoelectric signal is enhanced; the high-sensitivity and high-specificity detection on the 5hmC is realized. The detection method is simple and easy to operate and low in cost, not only expands the application range of the two nano materials, but also can realize detection of 5hmC.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical analysis, in particular to the preparation of a photoelectrochemical biosensor based on perovskite and black zirconia and its method for detecting 5-hydroxymethylcytosine deoxyribonucleotide (5hmC) method. Background technique [0002] 5hmC was first discovered in phage in 1952, but it did not attract enough attention at that time. Until 2009, Science reported that 5hmC is abundantly expressed in mammalian genomic DNA, and 5-methylcytosine (5mC) can be oxidized to 5hmC by the TET (ten-eleven translocation) enzyme family. The research has drawn a lot of attention. As the "sixth base", the function, genome distribution and biological function of 5hmC are being studied in depth. Notably, 5hmC levels were significantly reduced in various cancers, suggesting that 5hmC plays an important role in cancer development. In addition, abnormal distribution of 5hmC has also been found in patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N21/76G01N27/26G01N27/30G01N27/3278
Inventor 周云雷丁佳殷焕顺艾仕云方曦王茜
Owner SHANDONG AGRICULTURAL UNIVERSITY
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