Preparation method of artemisinin liposome loaded alginate-silk fibroin composite hydrogel

A technology of composite hydrogel and silk fibroin, which is applied in the field of preparation of alginate-silk fibroin composite hydrogel, can solve problems such as poor mechanical properties and limitations, and achieve good biological and mechanical properties and excellent performance , Improve the effect of physical defects

Active Publication Date: 2021-06-15
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biopolymers are generally limited due to their poor mechanical properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Preparation of artemisinin liposomes

[0028] Liposomes were prepared by ethanol injection. Dissolve 10ml of PC S100, cholesterol, SA and artemisinin at a ratio of 30:10:1:1 in 200ml of absolute ethanol. The solution was then slowly injected into phosphate buffered saline (PBS, pH 7.4) with a syringe, and the remaining ethanol was evaporated at 50°C with constant stirring. Then, the resulting liposome suspension was filtered twice through a 0.4 micron polycarbonate membrane. Finally, the artemisinin-loaded liposomes were stored under nitrogen protection until use.

[0029] 2) Preparation of freeze-dried silk fibroin

[0030] Cut 13 silkworm cocoons into pieces, boil them twice in 0.02M sodium carbonate solution at 40°C for 20 minutes each time; rinse thoroughly with deionized water. Degummed silk fibers were dissolved in CaCl with a molar ratio of 1:2:8 2 -Ethanol-H 2 In a ternary system of O, stir at 78 °C. The resulting fibroin was centrifuged for 15 min at ...

Embodiment 2

[0037] 1) Preparation of artemisinin liposomes

[0038] Liposomes were prepared by ethanol injection. Dissolve 15ml of PC S100, cholesterol, SA and artemisinin at a ratio of 30:10:1:1 in 250ml of absolute ethanol. The solution was then slowly injected into phosphate buffered saline (PBS, pH 7.4) with a syringe, and the remaining ethanol was evaporated at 50°C with constant stirring. Then, the resulting liposome suspension was filtered twice through a 0.4 micron polycarbonate membrane. Finally, the artemisinin-loaded liposomes were stored under nitrogen protection until use.

[0039] 2) Preparation of freeze-dried silk fibroin

[0040] Cut 15 silkworm cocoons into pieces, boil them twice in 0.02M sodium carbonate solution at 40°C for 20 minutes each time; rinse thoroughly with deionized water. The degummed silk fibers were dissolved in a ternary system of CaCl2-ethanol-H2O with a molar ratio of 1:2:8 and stirred at 78 °C. The resulting fibroin was centrifuged for 15 min at...

Embodiment 3

[0047] 1) Preparation of artemisinin liposomes

[0048] Liposomes were prepared by ethanol injection. Dissolve 20ml of PC S100, cholesterol, SA and artemisinin at a ratio of 30:10:1:1 in 300ml of absolute ethanol. The solution was then slowly injected into phosphate buffered saline (PBS, pH 7.4) with a syringe, and the remaining ethanol was evaporated at 50°C with constant stirring. Then, the resulting liposome suspension was filtered twice through a 0.4 micron polycarbonate membrane. Finally, the artemisinin-loaded liposomes were stored under nitrogen protection until use.

[0049] 2) Preparation of freeze-dried silk fibroin

[0050] Cut 17 cocoons into pieces, boil them twice in 0.02M sodium carbonate solution at 40°C for 20 minutes each time, and rinse them thoroughly with deionized water. The degummed silk fibers were dissolved in a ternary system of CaCl2-ethanol-H2O with a molar ratio of 1:2:8 and stirred at 78 °C. The resulting fibroin was centrifuged for 15 min at...

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Abstract

The invention relates to the field of biomedical materials, and discloses a preparation method of an artemisinin liposome-loaded alginate-silk fibroin composite hydrogel. The preparation method comprises the following steps that an artemisinin drug and other lipids are dissolved in ethanol according to a proper proportion by an ethanol injection method, and injecting and evaporating are carried out to prepare an artemisinin drug-loaded liposome; degumming is carried out by using a sodium carbonate solution, silk fibroin is stirred and dissolved by selecting a CaCl2-ethanol-H2O ternary system, and centrifugal dialyzing and freeze-drying are carried out to obtain silk fibroin; and sodium alginate solution is prepared, then a mixture is formed from drug-loaded liposome, silk fibroin and sodium alginate, and centrifugal ultrasonic treatment is carried out to prepare the hydrogel. The artemisinin liposome-loaded alginate-silk fibroin composite hydrogel has excellent drug controlled release ability and extremely high drug bioavailability, the hydrogel prepared by compounding sodium alginate and silk fibroin is further very good in biocompatibility, and the sodium alginate and the silk fibroin form synergistic complementation, so that the mechanical property of the silk fibroin hydrogel is improved.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing an alginate-silk fibroin composite hydrogel loaded with artemisinin liposomes. Background technique [0002] Hydrogel is a swollen three-dimensional three-dimensional network structure polymer. Hydrogel dressings with excellent performance can create a hypoxic environment on the wound, keep the affected part moist, and remove necrotic tissue and toxins. Hydrogels have an extracellular matrix-like polymer network that allows the loading of bioactive molecules. In addition to the advantages of hydrogels, drug-loaded hydrogels also have the advantages of targeted drug release, increased drug bioavailability, high sensitivity to biological environments, and promotion of wound tissue healing. [0003] Some drug delivery systems, such as nanoemulsions, nanoparticles, hydrogels, liposomes, have been shown to improve the controlled release of drugs. Especial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0066A61L26/0047A61L26/0023A61L2300/626A61L2300/216C08L89/00C08L5/04
Inventor 王秉邓明姚柳婷彭志勤万军民
Owner ZHEJIANG SCI-TECH UNIV
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