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Method for synthesizing medium-chain fatty acid from planting wastes

A medium-chain fatty acid and waste technology is applied in the field of resource utilization of planting waste to achieve the effects of reducing pollution, realizing resource utilization, and reducing costs.

Inactive Publication Date: 2021-06-15
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for synthesizing medium-chain fatty acids from planting waste, and propose a resource utilization method for producing ethanol, short-chain fatty acids, methane and MCFAs from planting waste, and solve the problem of medium-chain fatty acid produced by anaerobic fermentation of planting waste The process needs to add exogenous electron donors such as ethanol and lactic acid

Method used

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  • Method for synthesizing medium-chain fatty acid from planting wastes

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Embodiment 1

[0025] (1) Corn stalks were used as fermentation raw materials, cellulase and yeast were added to a 0.5L anaerobic fermentation bottle, and anaerobic fermentation was carried out at 30°C, TS 10%, and pH 5 to produce ethanol. After the cellulose is decomposed into glucose under the action of cellulase, the yeast decomposes the sugar into ethanol under anaerobic conditions.

[0026] (2) Distill and extract the ethanol in step (1) under heating conditions; then add livestock and poultry manure as inoculum to the anaerobic fermentation bottle in step (1), mix it with the remaining residue for anaerobic fermentation to generate biogas, Continue anaerobic fermentation to produce biogas at 35°C, TS (solid content) 20%, and pH 6.8;

[0027] (3) Add corn stalks and inoculated sludge containing acid-producing bacteria into another 0.5L anaerobic fermentation bottle, and carry out anaerobic fermentation to produce acid under the conditions of 25°C, TS 6%, and pH 5.5 to generate acetic ac...

Embodiment 2

[0030] (1) Corn stalks were used as fermentation raw materials, cellulase and yeast were added, and anaerobic fermentation was carried out at 35°C, TS 20%, and pH 6 to produce ethanol.

[0031] (2) Distill and extract the ethanol in step (1) under heating conditions, then add cow dung to the remaining fermentation residue as an inoculum, and continue anaerobic fermentation at 40°C, TS 25%, and pH 7 to produce biogas.

[0032] (3) Using corn stalks as fermentation raw materials, adding inoculated sludge rich in acid-producing bacteria, anaerobic fermentation was carried out at 30°C, TS 8%, and pH 6 to produce acid and produce short-chain fatty acids such as acetic acid.

[0033] (4) Ethanol obtained by distillation was added, and the carbon chain extension experiment was carried out at 40°C and pH 7, and the yield of MCFAs could reach 9.4g / L.

Embodiment 3

[0035] (1) Corn stalks were used as fermentation raw materials, cellulase and yeast were added, and anaerobic fermentation was carried out at 40°C, TS was 30%, and pH was 7 to produce ethanol.

[0036] (2) Distill and extract the ethanol in step (1) under heating conditions, then add cow dung to the remaining fermentation residue as an inoculum, and continue anaerobic fermentation at 45°C, TS 30%, and pH 7.2 to produce biogas.

[0037] (3) Using corn stalks as fermentation raw materials, adding inoculated sludge rich in acid-producing bacteria, anaerobic fermentation was carried out at 35°C, TS 10%, and pH 6.5 to produce acid and produce short-chain fatty acids such as acetic acid.

[0038] (4) Ethanol obtained by distillation was added, and the carbon chain extension experiment was carried out at 50°C and pH 8, and the yield of MCFAs could reach 8.7g / L.

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Abstract

The invention discloses a method for synthesizing medium-chain fatty acid from planting wastes, and belongs to the field of resource utilization of the planting wastes. The problem that exogenous electron donors such as ethanol and lactic acid need to be added in the process of producing the medium-chain fatty acid by anaerobic fermentation of the planting wastes is solved. The method comprises the following steps that corn straw is taken as a raw material, and cellulase and saccharomycetes are added for anaerobic fermentation to produce ethanol; the ethanol is extracted; the corn straw is used as a fermentation raw material, inoculated sludge rich in acid-producing bacteria is added, hydrolytic acidification is carried out under an anaerobic condition, and short-chain fatty acids such as acetic acid are generated; and ethanol obtained by distillation is added and used as an electron donor, and the medium-chain fatty acid is synthesized by extending a carbon chain under the action of clostridium kluyvei. Diversified utilization of the planting wastes is achieved, high-value utilization of the planting wastes is further achieved, and an economical and feasible method is provided for producing the medium-chain fatty acid through anaerobic fermentation of the planting wastes.

Description

technical field [0001] The invention belongs to the field of resource utilization of planting waste, and in particular relates to a method for synthesizing medium-chain fatty acids from planting waste. Background technique [0002] China is a large agricultural country, and a large amount of planting waste is produced every year. In 2019, the country's major crop straw production was 865 million tons, and the amount of straw that can be collected was 731 million tons. The main component of planting waste is lignocellulose, which is biodegradable. Therefore, with the help of biotechnology, planting waste is completely effective. Potential to be converted into bio-based products with higher added value. [0003] Crop straw is a biomass resource rich in carbon and hydrogen. The use of straw anaerobic fermentation to produce biogas is a way of energy utilization, which has been widely used in my country. However, there are problems such as long degradation cycle, low degradati...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12P7/10C12P7/54C12P7/40C02F11/04C12R1/145
CPCC12P5/023C12P7/10C12P7/54C12P7/40C02F11/04C12P2203/00Y02E50/10Y02E50/30
Inventor 赵立欣陈润璐于佳动罗娟姚宗路申瑞霞安柯萌
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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