Method for preparing five anthocyanin standard substances from blueberry anthocyanin extract

A blueberry anthocyanin and extract technology, which is applied in the directions of instruments, measuring devices, scientific instruments, etc., can solve the problems of poor separation, low purity and high production cost, and achieves large-scale preparation, simple and convenient operation. , the effect of large output
CN112964804AActive Publication Date: 2021-06-15GUIZHOU INST OF BIOLOGY
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
GUIZHOU INST OF BIOLOGY
Publication Date
2021-06-15

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Abstract

The invention relates to a method for preparing five anthocyanin standard substances from a blueberry anthocyanin extract, which comprises the following steps: treating a blueberry anthocyanin solution to be prepared containing five anthocyanin components through an organic filter membrane, and then carrying out semi-preparative high performance liquid chromatography treatment to obtain the five anthocyanin standard substances. The five kinds of anthocyanins are delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, peonidin-3-O-glucoside and malvidin 3-O-glucoside. According to the method, the semi-preparative high performance liquid chromatography treatment conditions are optimized, so that different anthocyanin substances can be collected at different retention times, the method for simultaneously preparing five main anthocyanin standard substances from the blueberry anthocyanin extract is realized, the purity is high, and the yield is high; and moreover, the operation is simple and convenient, and the large-scale preparation of the anthocyanin in blueberries and the demand of the market on the anthocyanin monomers can be met.
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Description

technical field

[0001] The invention belongs to the technical field of preparing standard anthocyanins, and in particular relates to a method for preparing five kinds of anthocyanin standard products from blueberry anthocyanin extracts. Background technique

[0002] Anthocyanins or anthocyanins are water-soluble natural pigments, and the basic structure core is 2-phenylbenzopyran, that is, Flavylium. There are more than 500 kinds of anthocyanins known in nature, of which 6 are the most common, namely cyanidin or hibiscus anthocyanin (Cyanidin), delphinidin or delphinidin, delphinidin ( Delphinidin), Peonidin, Petunidin or Petunidin, Malvidin and Pelargonidin. Anthocyanins generally exist in the form of glycosides, and often form anthocyanins or anthocyanins (Anthocyanin) through glycosidic bonds with one or more glucose, rhamnose, galactose, arabinose, etc., in which anthocyanin-3-O- Glucoside is the main anthocyanin.

[0003] Anthocyanins are a group of compounds that ca...

Claims

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