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Stem cell preparation for treating premature ovarian failure and application and preparation method thereof

A stem cell preparation and premature ovarian failure technology, applied in the field of biomedicine, can solve the problems that the effect needs to be improved, increase the incidence of breast cancer, cardiovascular disease, stroke, etc., and achieve better therapeutic effect

Active Publication Date: 2021-06-18
多能干细胞再生医学科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of premature ovarian failure mainly uses hormone therapy, but some clinical studies have found that hormone therapy for premature ovarian failure will increase the incidence of breast cancer, endometrial cancer, cardiovascular disease, and stroke
[0003]In recent years, mesenchymal stem cell transplantation has become a hot spot in clinical research, which provides new hope for the clinical treatment of premature ovarian failure and has achieved preliminary curative effect, but the effect is still to be seen. improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After primary culture, huMSCs (human umbilical cord mesenchymal stem cells) were placed in DMEM / F12 medium containing 10% FBS, 100 U / ml penicillin and streptomycin; then, at 37°C, 5% CO 2 The subcultured cells were routinely cultured and subcultured in an incubator; the subcultured cells were digested with 0.25% trypsin, and the stably proliferating cells were collected.

[0021] will be 10 6 The collected human umbilical cord mesenchymal stem cells were suspended in 100ml PBS buffer solution containing 1% bovine serum albumin, and positive antibodies (CD90, CD105, CD73) and negative antibodies (CD45, CD34, CD11b) were detected by flow cytometry. , CD19 and HLA-DR) expression. The results showed that the human umbilical cord mesenchymal stem cells cultured in Example 1 met the stem cell standard.

Embodiment 2

[0023] A stem cell preparation for treating premature ovarian failure, its components include umbilical cord mesenchymal stem cells prepared in Example 1, proline, 5-hydroxyfurfural, cyanidin-3-O-glucoside and physiological saline; The density of umbilical cord mesenchymal stem cells was 10 4 pieces / ml; the mass fraction of proline is 2wt%; the mass fraction of 5-hydroxyfurfural is 1wt%; the mass fraction of cyanidin-3-O-glucoside is 0.2wt%.

[0024] Dissolve proline, 5-hydroxyfurfural, and cyanidin-3-O-glucoside in physiological saline to make a compound solvent, and pre-cool at 4°C for later use; resuspend the umbilical cord mesenchymal stem cells prepared in Example 1 In the compound solution, vested.

Embodiment 3

[0026] A stem cell preparation for treating premature ovarian failure, its components include umbilical cord mesenchymal stem cells prepared in Example 1, proline, 5-hydroxyfurfural, cyanidin-3-O-glucoside and physiological saline; The density of umbilical cord mesenchymal stem cells was 10 3 each / ml; the mass fraction of proline is 1wt%; the mass fraction of 5-hydroxyfurfural is 2wt%; the mass fraction of cyanidin-3-O-glucoside is 0.1wt%.

[0027] Dissolve proline, 5-hydroxyfurfural, and cyanidin-3-O-glucoside in physiological saline to make a compound solvent, and pre-cool at 4°C for later use; resuspend the umbilical cord mesenchymal stem cells prepared in Example 1 In the compound solution, vested.

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PUM

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Abstract

The invention provides a stem cell preparation for treating premature ovarian failure. The stem cell preparation comprises the following components: umbilical cord mesenchymal stem cells, proline, 5-hydroxyfurfural, cyanidin-3-O-glucoside and normal saline, the density of the umbilical cord mesenchymal stem cells is 10 < 3 >-10 < 5 > / ml; the mass fraction of the proline is 1-5 wt%; the mass fraction of the 5-hydroxyfurfural is 0.5 to 2 weight percent; the mass fraction of the cyanidin-3-O-glucoside is 0.1 wt% to 0.5 wt%. The invention also provides application and a preparation method of the stem cell preparation. According to the stem cell preparation, the umbilical cord mesenchymal stem cells are matched with the proline, the 5-hydroxyfurfural and the cyanidin-3-O-glucoside, the treatment effect on the premature ovarian failure is better, and the cell preparation can maintain the activity of the umbilical cord mesenchymal stem cells for a long time, is free of any toxic and side components and is safe and effective.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a stem cell preparation for treating premature ovarian failure and its application and preparation method. Background technique [0002] Premature ovarian failure refers to a disease characterized by a decrease in serum estrogen levels and an increase in gonadotropin levels in women before the age of 40. According to epidemiological statistics, the incidence rate of women before the age of 40 is 1%, premature ovarian failure accounts for 10%-28% in primary amenorrhea, and premature ovarian failure accounts for 4%-18% in secondary amenorrhea, and the incidence rate is as follows: rising trend year by year. Premature ovarian failure has become one of the main causes of infertility in women today, seriously affecting the quality of life of patients and increasing social and economic burdens. The etiology and pathogenesis of premature ovarian failure are complex and not yet fully underst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P15/08A61K31/401A61K31/34A61K31/7048
CPCA61K35/28A61K31/401A61K31/34A61K31/7048A61P15/08A61K2300/00
Inventor 顾军
Owner 多能干细胞再生医学科技(广州)有限公司
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