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Electrospinning PLGA/PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration and preparation method thereof

A fiber membrane and chitosan technology, applied in the field of electrospun PLGA/PCL fiber membrane composite chitosan sponge scaffold and its preparation, can solve the problem of active adaptation to the shape of irregular bone defects without reversibility and the degradation speed of filling materials Need to be improved, not suitable for cell growth and other issues, to achieve the effect of preventing penetration, high biocompatibility, and less usage

Active Publication Date: 2021-06-18
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the material has low porosity, which is not suitable for cell growth, and its shape does not have reproducibility and active adaptation to irregular bone defect shapes, and the degradation rate of bone defect filling materials in this material also needs to be improved.

Method used

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  • Electrospinning PLGA/PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration and preparation method thereof
  • Electrospinning PLGA/PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration and preparation method thereof
  • Electrospinning PLGA/PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] S1: Dissolving PLGA and PCL in trifluoroethanol at a volume ratio of 1:1 to obtain the first mixed solution, the concentration of PLGA and PCL in the first mixed solution is 0.1g / mL; put the first mixed solution into the syringe, Placed in a microinjector, the injection speed is 0.5mL / h, the voltage of the electrospinning transformer is set to 20kv, the receiving distance is 15cm, the room temperature during electrospinning is 25°C, and the relative air humidity is 35%. The collected fiber membrane Freeze-dried and stored.

[0048] S2: Dissolve dopamine powder in Tri-HCl solution, stir for 30s to obtain a second mixed solution with a dopamine concentration of 10 mg / mL, the temperature of the second mixed solution is 20°C, and the area is 100-1000mm 2 , the fiber membrane obtained in step S1 with a thickness of 50-500 μm is soaked in the solution in the dark for 2 hours, and the room temperature during soaking is 25°C. After soaking, the fiber membrane is taken out and r...

Embodiment 2

[0053] S1: Dissolving PLGA and PCL in trifluoroethanol at a volume ratio of 2:1 to obtain the first mixed solution, the concentration of PLGA and PCL in the first mixed solution is 0.3g / mL; put the first mixed solution into a syringe, Placed in a microinjector, the injection speed is 0.7mL / h, the voltage of the electrospinning transformer is set to 25kv, the receiving distance is 20cm, the room temperature during electrospinning is 25°C, and the relative air humidity is 50%. The collected fiber membrane Freeze-dried and stored.

[0054] S2: Dissolve dopamine powder in Tri-HCl solution, stir for 60s to obtain a second mixed solution with a dopamine concentration of 15 mg / mL, the temperature of the second mixed solution is 30°C, and the area is 100-1000mm 2 , the fiber membrane obtained in step S1 with a thickness of 50-500 μm was soaked in the solution in the dark for 3 hours, and the room temperature during soaking was 25°C. After soaking, the fiber membrane was taken out and ...

Embodiment 3

[0058] S1: Dissolve PLGA and PCL in trifluoroethanol at a volume ratio of 3:1 to obtain the first mixed solution, the concentration of PLGA and PCL in the first mixed solution is 0.4 / mL; put the first mixed solution into a syringe, place In the microinjector, the injection speed is 0.8mL / h, the voltage of the electrospinning transformer is set to 20kv, the receiving distance is 15cm, the room temperature during electrospinning is 25°C, and the relative air humidity is 20%. The collected fiber membranes are frozen Store dry.

[0059] S2: Dissolve dopamine powder in Tri-HCl solution and stir for 60s to obtain a second mixed solution with a dopamine concentration of 10mg / mL. The temperature of the second mixed solution is 30°C and the area is 100-1000mm 2 , the fiber membrane obtained in step S1 with a thickness of 50-500 μm is soaked in the solution in the dark for 2 hours, and the room temperature during soaking is 25°C. After soaking, the fiber membrane is taken out and rinsed...

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Abstract

The invention discloses an electrospinning PLGA / PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration. The stent is provided with a compact layer and a loose layer which are tightly combined, the compact layer is an electrospinning polylactic acid-glycolic acid / polycaprolactone (PLGA / PCL) fiber membrane, and the fiber membrane has internal permeability and is composed of disordered fibers; the loose layer is a chitosan sponge and has a loose porous structure; and the porosity of the chitosan sponge ranges from 91.14% to 95.74%. The invention also discloses a preparation method of the electrospinning PLGA / PCL fiber membrane composite chitosan sponge stent for oral cavity alveolar bone regeneration. The stent disclosed by the invention has an integrated compact-loose structure, and the electrospinning compact layer has a supporting effect and can prevent penetration of fibroblasts; and the loose layer has good shape renaturation and water absorption effect, can be combined with blood to form gel, is very suitable for cell ingrowth, and is beneficial to bone tissue regeneration.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials, in particular to an electrospun PLGA / PCL fiber membrane composite chitosan sponge support for oral alveolar bone regeneration and a preparation method thereof. Background technique [0002] In the reconstruction of oral alveolar bone, the reconstruction of new bone and the invasion of soft tissue have been in a dynamic competition. Without intervention, the soft tissue is likely to preemptively occupy the bone defect area, so that the defect area cannot reach bone repair effect. At present, the clinical method to deal with the above problems in the oral cavity is: when using bone filling materials (such as Bio-Oss, hydroxyapatite, etc.), they are used together with barrier membranes (such as bio-gide, collagen membranes, etc.). Although this method allows enough time for the bone filling material to form new bone, the bone filling material in this method is easy to move, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/20A61L27/18A61L27/50
CPCA61L27/18A61L27/20A61L27/50A61L27/56A61L2400/16A61L2430/02C08L5/08C08L67/04
Inventor 章非敏陈汉帮胡姝颖孙上雯
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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