A graphene-based polymer composite nerve guide and preparation method thereof

Abstract

The invention provides a method for preparing a graphene-based polymer composite nerve guide, comprising the following steps: (1) Preparation of graphene oxide: first stage: prepare expanded graphite; second stage: prepare high-concentration graphene oxide Dispersion liquid; (2) Printing ink preparation: sodium alginate solution is printing ink 1; graphene dispersion liquid is added with ethylenediamine solution to be printing ink 2; (3) 3D printing rGO catheter: coaxial dual-material 3D printing needle is used, Ink 1 flows out through the main printing needle of the 3D printer, and ink 2 flows out through the auxiliary printing needle to form a macroscopic three-dimensional rGO conduit, which is freeze-dried and ready for use; (4) Preparation of graphene-based polymer tube: the rGO conduit is burned and reduced, and the The polymer was dissolved in dichloromethane, and the rGO catheter was soaked in the above liquid. After soaking for a period of time, the rGO catheter was taken out and air-dried under natural conditions. The graphene-based polymer composite nerve guide was prepared.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a graphene-based polymer composite nerve conduit and a preparation method thereof. Background technique [0002] Peripheral nerves play an important role in supporting structural stability in the motor and sensory nervous systems. Its destruction can lead to paralysis and loss of essential functions in patients. The number of peripheral nerve injuries is increasing every year, posing a great threat to human health. . At present, autologous nerve transplantation is the gold standard for the treatment of severe peripheral nerve defects. However, autologous transplantation has great limitations, such as severe shortage of donors, which will cause unavoidable secondary damage to the donor site. Therefore, artificial nerve repair catheter has become a hot spot of modern medical research. [0003] A good neural implant should have the following characteristics: 1. Good neu...

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Problems solved by technology

However, it is difficult for a single material to meet the above characteristics, so people have developed a variety of composite materials, among which the composite of polymer and graphene materials is particularly important

[0004] Graphene materials have been proven to have good biocompatibility and electrical conductivity, but at present, most of its applications in nerve conduits are based on other materials. Graphene is mixed into printing ink as an additive material, or coated as a coating on On the surface of nerve guides made of other materials, the carrying of the conductive network depends on the random stacking of graphene sheets. The porous structure mainly comes from the preparation process (electrospinning or printing molds, etc.), which greatly reduces the graphene The applicability and processability of materials in nerve tissue engineering materials, therefore, it is particularly necessary to develop a nerve guide with graphene three-dimensional network tubular structure as the main body and other polymer materials as auxiliary materials

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