Choline polysaccharide conductive nervous tissue engineering hydrogel material as well as preparation method and application thereof

A nerve tissue and hydrogel technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of inability to take into account excellent electrical conductivity and biological signal transduction function, lack of biological signal transduction ability, etc., and achieve the effect of high mechanical performance.

Active Publication Date: 2021-06-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing conductive nerve tissue engineering hydrogel materials cannot take into account the excellent conduction and biological signal conduction functions, resultin

Method used

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  • Choline polysaccharide conductive nervous tissue engineering hydrogel material as well as preparation method and application thereof
  • Choline polysaccharide conductive nervous tissue engineering hydrogel material as well as preparation method and application thereof
  • Choline polysaccharide conductive nervous tissue engineering hydrogel material as well as preparation method and application thereof

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Experimental program
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Effect test

specific Embodiment approach 1

[0066] DETAILED DESCRIPTION OF THE INVENTION An Embodiment of the Cholinergic Polysaccharide Conductive Nerve Tissue Engineering Handan Base is introduced into a carboxyl group in the chitosan C6-glycosyl hydroxyl. Then the carboxymethyl chitosan is reacted with 3,4-dihydroxybenzaldehyde, and the phosphol of the phosphol is obtained, and the choline is grafted to the phyttolic carboxyme-chollane Sugar C6-based carboxy group to obtain choline-phylfocardial carboxyme-chitosan, and finally add pyrrole, iron chloride and iron chloride and iron chloride and iron chloride and iron chloride, and Calcium chloride, using a phenolic hydroxyl group and iron ion coordination, carboxyl group and calcium ion coordination, and hydrogen bond between polypyrrole and polysaccharide to obtain choline polysaccharide conductive neurotrophic engineering hydrogel materials.

[0067] The phthal-phytosyl chitin of the phytosyltoxy of the present embodiment contains a bacteriostatic Schiff base structure, ...

specific Embodiment approach 2

[0069] DETAILED DESCRIPTION OF THE INVENTION The present embodiment and the specific embodiment are different: the preparation method of the choline is done by following:

[0070] 250 μl of 2-amino ethanol was added to 50 ml of three-neck flask, then 20 ml of anhydrous methanol and 2G anhydrous K 2 CO 3 Nitrogen gas in the tri-necked flask is then added to the nitrogen atmosphere and magnetic stirring conditions, and finally at a nitrogen atmosphere, at room temperature and stirring conditions for 10 h, the reaction product is obtained; the reaction product is in an oven at 25 ° C 12h was dried to give 2-hydroxy-N ', N', N'-trimethylthylamine, which is choline. Other steps are the same as specific embodiments.

specific Embodiment approach 3

[0071] DETAILED DESCRIPTION OF THE INVENTION 3: The present embodiment is a method for preparing a choline polysaccharide conductive neurotrophic engineering hydrogel material to be completed as follows:

[0072] First, prepare a carboxymethyl chitcan:

[0073] 1, the chitosan and sodium hydroxide are added to the aqueous isopropyl alcohol, and then swollen, alkalized to give a water solution of the chitosan;

[0074] 2, dissolve chloroacetic acid in isopropyl alcohol to obtain a solution of isopropyl alcohol of chloroacetic acid;

[0075] 3, adding the isopropyl alcohol solution of chloroacetic acid to the isopropyl alcohol solution of churosan to obtain a reaction liquid I; a stirring reaction to the reaction liquid, and finally the reaction is added to the reaction, and then filtrate, resulting in a solid reaction Product I;

[0076] 4, washed the solid reaction product to obtain a dehydrated desalination product; a dehydrated desalination product was vacuum dried at room tempe...

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Abstract

The invention discloses a choline polysaccharide conductive nervous tissue engineering hydrogel material as well as a preparation method and the application thereof, and relates to conductive nervous tissue engineering polysaccharide hydrogel and a preparation method thereof. The invention aims to solve the problem that the existing conductive nervous tissue engineering hydrogel material cannot give consideration to excellent conductivity and biological signal transduction functions, so that the conductive nervous tissue engineering hydrogel material is poor in biological signal transduction capability. The preparation method comprises the following steps: 1, preparing carboxymethyl chitosan; 2, preparing catechol carboxymethyl chitosan; 3, preparing choline-catechol carboxymethyl chitosan; and 4, preparing the choline polysaccharide conductive nerve tissue engineering hydrogel material. The choline polysaccharide conductive nervous tissue engineering hydrogel material is used as a nervous tissue engineering material for promoting defective nerve repair. The choline polysaccharide conductive nerve tissue engineering hydrogel material can be obtained.

Description

Technical field [0001] The present invention relates to a polysaccharide hydrogel of conductive nerve tissue and a preparation method thereof. Background technique [0002] Nerve injury repair is a major research problem facing the regenerative medicine. Autologous nerve transplantation is the gold standard for treating peripheral nerve injury, but health perceived neurons caused additional wounds and sensory loss, and limited donor nerve is difficult to meet a wide range of nerve injury repair sources . Nerve repair is a dynamic complex process, relying on cellular signal factor adjustment, thereby achieving efficient and ideal repair effects, exploring cellular signal factors conducive to nerve repair is an urgent problem to be solved in the development of neurological engineering materials. Choline can be used as a protective nerve, maintaining neuroplastic signal molecular transmission nerve cell information, and regulates local micro-environment balance around the damaged ne...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/52A61L27/54A61L27/50C08L5/04C08L5/08C08L79/04C08J3/075C08J3/24C08K3/24C08B37/08
CPCA61L27/18A61L27/20A61L27/52A61L27/54A61L27/50C08J3/246C08J3/075C08B37/003C08J2305/04C08J2405/08C08J2479/04C08K3/16C08K2003/162A61L2430/32A61L2300/232A61L2300/404C08L5/04C08L5/08C08L79/04
Inventor 王路刘延浩李书彬王振宇
Owner HARBIN INST OF TECH
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