Living microalgae composition and application thereof in nutrient enrichment artemia and litopenaeus vannamei juvenile shrimp feeding
A composition and the technology of vannamei are applied in the application field of juvenile shrimp breeding of Litopenaeus vannamei, which can solve the problems such as the influence of seedling production, difficulty in cultivating shrimp seedlings, deterioration of water quality, etc. The effect of content and nutritional components fortification
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[0032] The present invention will be further described below in conjunction with specific examples.
[0033] Schizochytrium l imacinum ATCC 1381.
[0034] Chaetoceros gracilis was provided by Ningbo Futian Biotechnology Co., Ltd.
[0035] Litopenaeus vannamei larvae were provided by Guangdong Hisenong Group Co., Ltd.
[0036] Artemia (Artemia sp.) is brine shrimp eggs purchased from Crown brand, produced in the Tibetan plateau.
[0037] Litopenaeus vannamei larvae (Litopenaeus vannamei) were provided by Guangdong Hisenong Group Co., Ltd.
[0038] 1. The cultivation of microalgae
[0039] 1.1. The f / 2 medium is sterilized by filtration with a 0.22 μm fiber membrane, and the formula of the f / 2 medium is shown in the table below
[0040] Table 1 medium formula
[0041]
[0042] Schizochytrium fermentation medium: glucose 50g·L -1 , yeast extract 5g·L -1 , sodium glutamate 4g·L -1 , 25‰ of natural seawater, pH 6.8.
[0043] 1.2. Cultivation method of Chaetoceros tenaci...
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