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A Quantitative Detection Method for Meat Adulteration Based on Elemental Analysis

A quantitative detection method and elemental analysis technology, applied in the direction of analyzing materials, material analysis by electromagnetic means, measuring devices, etc., can solve the problems of low sensitivity, cumbersome operation, high cost, and achieve strong anti-interference ability, low operating cost, Detecting fast effects

Active Publication Date: 2021-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunological detection method is a highly selective analysis method established by using the specific reaction between antigen and antibody. Although it has high specificity, it cannot be quantitatively detected.
Chromatography-mass spectrometry is an efficient qualitative and quantitative detection of specific proteins in meat products, but the analysis cost is expensive and requires a high level of operator skills
Although infrared spectroscopy technology can quickly and non-destructively detect samples, substances with low content have weak absorption in the near-infrared region and low sensitivity
Electronic nose and electronic tongue technologies are easily affected by additional flavors and fragrances, and the external environment has a greater influence
Nucleic acid PCR technology is to analyze and detect the nucleotide sequence in the target object. Although it has high accuracy, the operation is cumbersome, costly and time-consuming.

Method used

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  • A Quantitative Detection Method for Meat Adulteration Based on Elemental Analysis
  • A Quantitative Detection Method for Meat Adulteration Based on Elemental Analysis
  • A Quantitative Detection Method for Meat Adulteration Based on Elemental Analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Sampling: 100 pork hind legs and 100 chicken breast samples were purchased from the market, and the pork and chicken were ground into uniform minced meat with a meat grinder, and stored at 4°C.

[0048] (2) Sample preparation: Weigh chicken meat according to the mass ratio of 0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 60%, 70% %, 80%, 90%, and 100% gradients were added to pork mince, and mixed thoroughly to simulate the adulteration of chicken into pork samples.

[0049] Digestion: 10 parallels for each sample. Take 0.5g of each simulated sample into a 50ml centrifuge tube, add 5ml of MOS grade concentrated nitric acid, place it in a heavy metal graphite digestion apparatus, set 120°C for 40 minutes, add 2ml of GR grade hydrogen peroxide and continue digestion for 30 minutes. Dilute to 50 ml with ultrapure water to be tested. The same digestion method prepared 10 blank samples to be tested.

[0050] The working parameters of the instrument are shown in ...

Embodiment 2

[0068] On the basis of Partial Least Squares Discriminant Analysis in Example 1, factor analysis: due to the large number of element variables involved in the element analysis method, and there may be intricate relationships between the variables, the factor analysis method is used to convert multiple observation elements Reflect the characteristics and essence of the whole research for a few elemental factors;

[0069] The first step is to standardize the quantitative analysis data of all elements, and treat the processed data as all element variables;

[0070] Do KMO and Bartlett tests on all element variables to judge whether they are suitable for factor analysis. The results are shown in Table 3. The KMO statistic is 0.78, which is greater than the minimum standard of 0.5, which is suitable for factor analysis; Bartlett spherical test rejects unit correlation matrix The null hypothesis of , P<0.001, suitable for factor analysis;

[0071] table 3

[0072]

[0073] The ...

Embodiment 3

[0091] Multiple linear regression analysis:

[0092] Regression analysis is a statistical analysis method used to determine the quantitative relationship between variables interdependence. This method is by carrying out multiple linear regression analysis to 12 kinds of extraction factors in the above-mentioned embodiment 2, draws the linear regression figure such as figure 2 shown.

[0093] At the same time, the linear equation 1 is obtained as follows:

[0094] Y=64.706-0.001X1+2.863e -5 X2-0.007X3-0.001X4-4.002e -7 X5-3.475e -5 X6+0.34X7-0.008X8-0.005X9+0.012X10+24.187X11-0.006X12

[0095]In the formula: X1: Ba (barium) content; X2: Ca (calcium) content; X3: Cu (copper) content; X4: Fe (iron) content; X5: K (potassium) content; X6: Mg (magnesium) content; X7: Mo (molybdenum) content; X8: Se (selenium) content; X9: Sr (strontium) content; X10: Tl (thallium) content; X11: Ti (titanium) content Content; X12: Zn (zinc) content; all content units are ng / g.

[0096] Veri...

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PUM

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Abstract

The invention discloses a method for quantitative detection of meat adulteration based on elemental analysis, which comprises: weighing minced chicken meat and adding it to minced pork meat, mixing thoroughly and uniformly to obtain a sample of simulated chicken adulterated into pork; adulterating simulated chicken into pork The pork sample was digested to obtain the digestion solution to be tested; the digestion solution to be tested was analyzed and detected to detect 23 elements; by detecting 23 common elements in 170 simulated samples, the online dilution internal standard method was used for accurate quantitative analysis Select 12 kinds of elements that cumulative contribution rate reaches 95% by factor analysis as extraction factor, be element Ba, Ca, Cu, Fe, K, Mg, Mo, Se, Sr, Tl, Ti, Zn respectively; By said 12 kinds of factors were extracted, and multiple linear regression analysis was carried out to obtain a linear equation. Based on the elemental analysis and detection technology, the present invention can realize the advantages of simple sample pretreatment, low operation cost, fast detection speed, good stability, strong anti-interference ability, etc., and can realize qualitative and quantitative detection and analysis at the same time.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a quantitative detection method for meat adulteration based on elemental analysis. Background technique [0002] Among the many food safety incidents, pork adulteration has always been an important issue of concern. With the development of society and the improvement of economic level, the intake of pork has become an indispensable part of people's daily diet. In order to better protect the interests of consumers and a fair competition environment, the research and development of rapid detection methods for adulterated meat is also extremely important. [0003] At present, in response to the adulteration of meat, countries have formulated some corresponding industry standards and adopted corresponding control methods. The main detection technologies are based on immunology technology, protein chromatography mass spectrometry technology, infrared spectroscopy, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/626G01N33/12G01N1/38G01N1/44
CPCG01N27/626G01N33/12G01N1/38G01N1/44
Inventor 高雅慧李子杰
Owner JIANGNAN UNIV