Composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and preparation method and application thereof

A composite material, free radical technology, applied in pharmaceutical formulations, medical science, prostheses, etc., can solve problems such as ROS that have not yet been developed, and achieve good mechanical properties and biocompatibility, good stability, and excellent biocompatibility sexual effect

Active Publication Date: 2021-06-29
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that inhibiting ROS can inhibit the occurrence of inflammatory reactions and promote tissue repair, but materials that effectively regulate ROS have not yet been developed.

Method used

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  • Composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and preparation method and application thereof
  • Composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and preparation method and application thereof
  • Composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example relates to hMnO 2 The preparation method of PLA spinning fiber support, comprises the following steps:

[0035] S11. Add 14 mL of absolute ethanol, 2 mL of deionized water, and 500 μL of ammonia water to the round-bottomed flask in a water bath at 50°C, and stir magnetically for 5 minutes until the liquid is evenly mixed to obtain a mixed liquid; Slowly add 500 μL of tetraethoxysilane dropwise into the above mixture through the funnel, continue to stir for 2 hours after the dropwise addition, then centrifuge at 14800 rpm for 5 minutes, remove the supernatant, and wash the precipitate twice with ethanol and deionized water respectively to obtain mesoporous SiO 2 Nanoparticles;

[0036] S12, the mesoporous SiO obtained in step S11 2 Nanoparticles were resuspended in 10 mL of deionized water, and 20 mL of KMnO was added dropwise under ultrasonic conditions. 4 Aqueous solution (KMnO 4 The total content is 600mg), continue to sonicate for 1h, then stir overn...

Embodiment 2

[0042] This embodiment relates to the preparation method of mPDA / GelMA composite hydrogel, comprising the following steps:

[0043] S21, add 14mL of absolute ethanol, 90mL of deionized water, 2mL of ammonia water to the round bottom flask, stir magnetically for 5min until the liquid is evenly mixed to obtain a mixed solution; then slowly add 10mL of concentration It is a dopamine solution of 50mg / mL. After the dropwise addition is completed, continue to stir for 1d, then centrifuge at 14800rpm for 5min, remove the supernatant, and wash the precipitate with deionized water 3 times to obtain mPDA nanoparticles; the TEM photo of mPDA nanoparticles is as follows image 3 shown;

[0044] S22, add 10mL of PBS to 0.5g of GelMA solid sponge, then place it in a 37°C water bath to dissolve to a transparent liquid, then add 0.025g of photoinitiator phenyl-2,4,6-trimethylbenzyl Lithium acylphosphonate is dissolved to obtain a GelMA solution with a mass concentration of 5%; 10 mg of mPDA ...

Embodiment 3

[0047] This example relates to mPDA / CaPO 4 The preparation method of composite bone cement comprises the following steps:

[0048] S31, add 14mL of absolute ethanol, 90mL of deionized water, 2mL of ammonia water to the round bottom flask, and magnetically stir for 5min until the liquid is evenly mixed to obtain a mixed solution; then slowly add 10mL of concentration It is a 50 mg / mL dopamine solution. After the dropwise addition is completed, continue to stir for 1 d, then centrifuge at 14800 rpm for 5 min, remove the supernatant, and wash the precipitate with deionized water 3 times to obtain mPDA nanoparticles;

[0049] S32, adding the mPDA mesoporous nanoparticles prepared in step S31 to a certain amount of calcium phosphate cement powder, and then placing it on a dry glass plate; then adding a corresponding amount of disodium hydrogen phosphate solution with a mass concentration of 0.25%, stirring, Obtain cement slurry; then put the cement slurry into a mold, place it at ...

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Abstract

The invention provides a composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The composite material capable of regulating and controlling the reactive oxygen species comprises an ROS scavenging matrix and a matrix carrier, wherein the ROS scavenging matrix is at least one of hMnO2 and mPDA; and the matrix carrier is a biocompatible material. The composite material prepared by the method can regulate and control the ROS, and can further promote tissue repair by releasing a loaded medicament and / or a growth factor after the medicament and / or the growth factor are / is loaded.

Description

technical field [0001] The invention relates to a technology in the field of biomedical materials, in particular to a composite material capable of regulating active oxygen radicals and its preparation method and application. Background technique [0002] After the tissue is damaged, a series of changes will occur inside it. For example, the pH of the damaged site will change from neutral or slightly alkaline to slightly acidic. The acidic environment will cause tissue acidosis and affect the tissue healing process; It will be accompanied by blood vessel rupture, resulting in insufficient blood supply to the damaged site, resulting in a drop in oxygen content at the damaged site, forming a hypoxic environment and producing a large amount of ROS (reactive oxygen species, active oxygen free radicals), a large amount of ROS will damage cellular nucleic acid and Lipids, induce apoptosis. Studies have shown that inhibiting ROS can inhibit the occurrence of inflammatory reactions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/02A61L27/18A61L27/50A61L27/54A61L27/52A61L27/56A61L27/22
CPCA61L27/025A61L27/18A61L27/50A61L27/54A61L27/52A61L27/56A61L27/222A61L2430/02A61L2430/32A61L2400/12A61L2300/414C08L79/04C08L67/04
Inventor 李斌李家颖韩凤选
Owner SUZHOU UNIV
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