Procyanidine plant nutrient and application thereof
A technology for plant nutrients and proanthocyanidins is applied in the field of crops to achieve the effects of simple preparation process, low cost and promoting plant growth
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Embodiment 1
[0042] Embodiment 1 uses pine needles as raw material to prepare proanthocyanidin plant nutrient
[0043] (1) Take fresh massoniana pine needles, crush them for later use. Add tap water with 10 times the mass of pine needle raw material, soak at 70°C for 36 hours, release the first extract after filtering; add water with 5 times the mass of pine needle raw material to the filter residue, soak at 70°C for 24 hours, and release the second extract after filtering Extraction solution; add water with 3 times the quality of the pine needle raw material to the filter residue, soak at 70°C for 12 hours, filter and combine three extraction solutions, concentrate to make an aqueous solution, and make the mass fraction of the active ingredient proanthocyanidins 0.01%; thus prepared Get Pine Needle Phytonutrients.
[0044] (2) or get fresh masson pine pine needles, add after crushing 40% ethanol aqueous solution with a volume fraction of 10 times the mass of the pine needle raw material,...
Embodiment 2
[0045] The detection of the proanthocyanidins in embodiment 2 plant nutrients
[0046] Take pine needle plant nutrition as an example. The 4,8 linkages between the proanthocyanidin structural units are easy to crack under the action of acid, the lower unit forms catechin or epicatechin, the upper unit becomes a carbocation, loses a proton to form a flavon-3-en-3-ol, and Then anthocyanins are formed. Based on this principle, UV-visible spectrophotometer method can be used to measure the absorbance of the generated product and carry out qualitative and quantitative detection of proanthocyanidins. Adopt n-butanol / hydrochloric acid method, get the pine needle phytonutrient in embodiment 1, be made into the methanol solution that pine needle phytonutrient concentration is 10mg / mL, get 1mL and add in the pressure-resistant glass reaction tube of 15mL, then add 0.2mL successively Ferric ammonium sulfate solution (2% mass fraction) and 3.8mL n-butanol / hydrochloric acid solution (95:...
Embodiment 3
[0047] Example 3 Preparation of proanthocyanidin plant nutrient with pine bark as raw material
[0048] Take the dry bark of slash pine and crush it for later use. Add 8 times the mass fraction of raw material 70% edible alcohol, soak at room temperature for 48 hours, filter and release the first extract; add 4 times the mass fraction of 70% edible alcohol, soak at room temperature for 24 hours, combine twice The extract was concentrated to obtain the total extract, so that the mass fraction of the active ingredient was 30%; thus the pine bark plant nutrient was prepared for testing in subsequent examples.
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