Extraction process for ultrasonic-assisted flash extraction of sweet osmanthus hydrolat and activity determination method

A flash extraction, ultrasonic-assisted technology, applied in the field of daily chemical products

Pending Publication Date: 2021-07-06
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the studies on the activity of pure dew focus on antibacterial properties, and most of the cognition of other efficacy comes from ancient book records, practical experience and even analogy inference. Assay method steps

Method used

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  • Extraction process for ultrasonic-assisted flash extraction of sweet osmanthus hydrolat and activity determination method
  • Extraction process for ultrasonic-assisted flash extraction of sweet osmanthus hydrolat and activity determination method
  • Extraction process for ultrasonic-assisted flash extraction of sweet osmanthus hydrolat and activity determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Extraction of sweet-scented osmanthus hydrosol by ultrasonic-assisted flash distillation:

[0039] 1. Experimental equipment and medicines:

[0040] 3000mL beaker, 5000mL flask, spherical condenser, Clevenger apparatus, glass rod, glass beads, sterile bottle,

[0041] Flash extractor (JBHT-50T, Henan Zhijing Biotechnology Co., Ltd.), ultrasonic cell crushing (Scientz-IID, China Ningbo Xinzhi Biotechnology Co., Ltd.), sterile low-speed filter paper, ultra-clean bench; electronic balance PL203 ( Mettler-Toledo Instruments (Shanghai) Co., Ltd.).

[0042] 2. Osmanthus fragrans hydrosol extraction steps:

[0043] 1) Soak 100g of sweet-scented osmanthus flowers dried quickly at 70°C in 1000mL of salt water;

[0044] 2) The material in step 1) is first extracted with 15V voltage flash for 30s;

[0045] 3) After stirring evenly up, down, left, and right, place the material in an ultrasonic instrument and sonicate 3 times with 600W power, 10 minutes each time.

[0046] 4) Put...

Embodiment 2

[0049] Determination of antioxidant activity of sweet-scented osmanthus hydrosol:

[0050] 1. Experimental equipment and medicines:

[0051] Ultraviolet spectrophotometer UV-6000 (Shanghai Yuanyi Instrument Co., Ltd.); microplate reader (Multiskan FC, Thermo Fisher Technology Co., Ltd., China); 96-well plate; absolute ethanol, 1,1-diphenyl- 2-Picrylic hydrazino (free radical) (DPPH), 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, dihydrogen phosphate Sodium, sodium dihydrogen phosphate (Shanghai Titan Technology Co., Ltd.).

[0052] 2. Experimental steps:

[0053] According to the double dilution method, dilute the pure dew to the following sample concentration:

[0054] Q (pure sample), 0.5Q, 0.25Q, 0.125Q, 0.0625Q;

[0055] 1) DPPH free radical scavenging experiment

[0056] Each sample was mixed with 0.16 mM DPPH solution (diluted with absolute ethanol) in a 96-well plate, and placed at 30°C in the dark. After 30 min...

Embodiment 3

[0067] In vitro Tyrosinase Activity Inhibition Experiment of Osmanthus fragrans Hydrosol

[0068] 1. Experimental equipment and medicines:

[0069] Ultraviolet spectrophotometer UV-6000 (Shanghai Yuan Analysis Instrument Co., Ltd.); microplate reader (Multiskan FC, Thermo Fisher Technology Co., Ltd., China); constant temperature water bath, ultrasonic cleaner, pipette gun, volumetric flask, 96 Orifice plates, centrifuge tubes; tyrosinase from mushrooms (Shanghai Merrill Chemical Technology Co., Ltd.), kojic acid, PBS (pH=6.8), L-DOPA, absolute ethanol (Shanghai Titan Technology Co., Ltd.), super pure water.

[0070] 2. Experimental steps:

[0071] Add 100 μL, 150 μL, 150 μL, and 200 μL of PBS (pH = 6.8) to wells A, B, C, and D in sequence. Add 50 μL of each sample to wells A and B respectively, then put 50 μL of tyrosinase solution (50 U / mL) into wells A and C, and react at 37°C for 10 minutes. Quickly add 100 µL of L-DOPA (5 mM) solution to the quadruple wells. After con...

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Abstract

The invention discloses an extraction process for ultrasonic-assisted flash extraction of sweet osmanthus hydrolat and an activity determination method. The method comprises the following steps: soaking osmanthus flowers in a saline solution, performing ultrasonic treatment after flash crushing, adding water for direct distillation, and receiving osmanthus hydrolat, wherein the sweet-scented osmanthus hydrolat is aromatic water with rich sweet-scented osmanthus fragrance and terpene component smell, is prominent in sweetness after being aged, and has pleasant fruit sweetness like peaches. According to the method disclosed by the invention, more active substances in petals are brought into a solution through the strong crushing force of the flash extractor, the cavitation effect generated between the rotary vane and liquid and the crushing effect of ultrasonic waves on plant cell walls, so that the additional value of osmanthus fragrans products is improved; in addition, an in-vitro and cell whitening experiment, an antioxidant determination method and experimental data of the sweet osmanthus hydrolat are provided, and it is proved that the sweet osmanthus hydrolat is extremely low in cytotoxicity and has antioxidant activity and whitening activity.

Description

technical field [0001] The invention belongs to the field of daily chemical products and relates to an osmanthus fragrans pure dew, in particular to an extraction process for ultrasonic-assisted flash extraction of osmanthus fragrans pure dew, a method for measuring anti-oxidation and whitening activities and basic data. Background technique [0002] Osmanthus fragrans (Osmanthus fragrans) is evergreen shrubs or dungarunga of Oleaceae, one of the top ten traditional famous flowers of China. Osmanthus osmanthus has a pleasant fragrance, but when it is thick, it will overflow far away. It can be eaten or used as medicine. Osmanthus fragrans is warm and pungent in nature, warms the stomach and relieves pain, can reduce phlegm and blood stasis, and has curative effects on loss of appetite, anti-caries, and abdominal pain after amenorrhea; osmanthus essential oil, extract, and absolute oil are favored in the fields of fragrance and aromatherapy. In recent years, pure dew, a by-p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N21/31
CPCG01N1/4022G01N21/31G01N2001/4033
Inventor 易封萍吕晨媛郝临雨陈梓谦苏畅张松幸
Owner SHANGHAI INST OF TECH
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