Talaromyces wortmannii, microbial inoculum and application of talaromyces wortmannii in preparation of growth regulator
A plant growth regulator and tallow bacteria technology, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of high cost and insignificant effect, and achieve the improvement of growth rate, remarkable effect, and easy labor. The effect of cultivation
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Embodiment 1
[0110] This example provides the screening, isolation and identification methods of Talaromyces wortmannii MN122989 strain.
[0111] The strain was isolated from the rhizosphere soil and named as Watermania trachobacterium MN122989.
[0112] The genome of the strain was extracted using the Biospin Fungal Genomic DNA Extraction Kit, and amplified with the primers shown in Table 1 below. The amplified product was sent to Jinweizhi Biotechnology Co., Ltd. for sequencing.
[0113] Table 1 PCR upstream and downstream primers.
[0114]
[0115] The sequencing results of ITS, BenA and CMD sequences are shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3.
[0116] The sequence sequencing results were compared for homology by Blast analysis. Phylogenetic tree analysis confirmed that the strain was Talaromyces wortmannii. Phylogenetic tree analysis chart reference figure 1 shown.
Embodiment 2
[0118] In this example, the fermentation broth of Talaromyces wortmannii MN122989 strain was prepared.
[0119] The preparation method includes: punching 18 pieces of bacteria with a sterile puncher, putting 18 pieces of bacteria into a 50mL sterile centrifuge tube containing 30mL of PDB culture solution, placing them in a constant temperature shaking incubator, at 28°C, 200rpm Under culture for 4 days, count the number of spores with a hemocytometer, and adjust the number of spores in the fermentation broth to 10 6 CFU / mL.
Embodiment 3
[0121] In this example, the research on the effect of the Wortmanella trachobacterium MN122989 strain on the drought resistance of maize seeds in the germination stage was carried out. Technical route reference figure 2 shown.
[0122] Disinfect the surface of the seeds first: take out the corn (Zhengdan 958) seeds from the cold storage, select healthy seeds with the same particle size, disinfect them with 75% ethanol for 1 minute, wash them with sterile water for 3 times, and then disinfect them with 5% sodium hypochlorite for 5 minutes. Finally, wash 3 times with sterile water.
[0123] Then carry out the seed soaking treatment: at room temperature, use each treatment solution to soak the seeds overnight. Use the fermented broth of the screening strain prepared in Example 2 to soak the seeds as the treatment group; use the PDB culture broth to soak the seeds as the negative control group. The blank control group was soaked with PDB culture solution, and watered normally ...
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