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A dna-binding antimicrobial peptide vpdb40 of Penaeus vannamei and its application

An antibacterial peptide, white shrimp technology, applied in the application, antibacterial drugs, peptides and other directions, can solve the problem that there is no nucleic acid sequence, recombinant protein research and application, etc.

Active Publication Date: 2022-06-28
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In Penaeus vannamei or other crustaceans, many antimicrobial peptide genes have been studied and reported, but the present invention has discovered a novel DNA-binding antimicrobial peptide during the research process, and there is no research on its nucleic acid sequence, recombinant protein and Application related reports

Method used

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  • A dna-binding antimicrobial peptide vpdb40 of Penaeus vannamei and its application
  • A dna-binding antimicrobial peptide vpdb40 of Penaeus vannamei and its application
  • A dna-binding antimicrobial peptide vpdb40 of Penaeus vannamei and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Mass spectrometry analysis of Penaeus vannamei DNA-bound antimicrobial peptides

[0042] First, the samples of Penaeus vannamei were prepared, and 400 healthy Penaeus vannamei were divided into high-intensity ultrasound group, medium-intensity ultrasound group, low-intensity ultrasound group and control group with 100 each. Each shrimp was injected with 0.1 mL of Vibrio parahaemolyticus (1.0×10 5-6 cfu / mL) or an equivalent amount of sodium chloride. Live shrimp were collected 24 hours after infection and minced immediately with PBS buffer. After centrifugation at 100g for 15min, the supernatant was taken and passed through a 3000Da filter. Store at -20°C for further analysis.

[0043] Chromatography was then performed using a Nano Aquity UPLC system (Waters Corp.) instrument. The injection volume was 5.0 μl, and the mobile phases were 0.1% methanol in water and acetonitrile, respectively. The elution gradients are shown in Table 1.

[0044] Table 1. Anti...

Embodiment 2

[0060] Example 2 Minimum inhibitory concentration (MIC) determination

[0061] Vibrio parahaemolyticus was incubated at 37°C for 12h to logarithmic growth phase and diluted to 10 in 0.01M pH 7.2 phosphate buffer 6-7 CFU / mL. The peptides were dissolved in phosphate buffer and mixed with bacteria in equal volume at 37°C for 2h. The minimum inhibitory concentration (MIC) is the lowest concentration of antimicrobial peptide at which no bacterial growth can be seen from the microtiter plate after incubation at 37°C. like figure 2 As shown, the minimum inhibitory concentration (MIC) of VPDB40 against Vibrio parahaemolyticus was 1.95 μg / mL.

Embodiment 3

[0062] Example 3 Time-kill curve (Time kill) determination

[0063] The time-kill curve of peptide VPDB40 was determined using the plate colony count method. Vibrio parahaemolyticus was incubated at 37°C for 12h to logarithmic growth phase and diluted to 10 in 0.01M pH 7.2 phosphate buffer 6-7 CFU / mL. Peptides were dissolved in phosphate buffer and diluted to 3.91 μg / mL, an equal volume was mixed with bacteria and incubated at 37°C. At different time points (ie 0.5, 1, 1.5, 2, 2.5 and 3 hours), 0.2 mL of bacterial suspension was obtained and colonies were counted after culturing on nutrient broth plates at 37°C for 24 hours. like image 3 shown.

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Abstract

The invention discloses a DNA binding antibacterial peptide VPDB40 of Penaeus vannamei, the amino acid sequence of which is GITIQCILPGFVVSKLSKLK, and the molecular weight of the antibacterial peptide is 2144.686 Daltons. The antibacterial peptide of the invention can effectively inhibit the growth of Vibrio parahaemolyticus, and can be used as a medicine for preventing or inhibiting Vibrio parahaemolyticus infection. The invention lays a foundation for further research on the Penaeus vannamei antimicrobial peptide as a food preservative and a feed additive for preventing shrimp diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DNA-binding antimicrobial peptide VPDB40 of Penaeus vannamei and application thereof. Background technique [0002] As the main shrimp culture species in my country, Penaeus vannamei has high economic value. In recent years, due to the deterioration of the breeding environment and the excessive breeding density, the frequent occurrence of diseases has seriously threatened the healthy development of the Penaeus vannamei industry and caused huge economic losses. Therefore, it is particularly important to carry out the prevention and control of shrimp diseases. [0003] In recent years, a mysterious disease called "Shrimp Early Death Syndrome" has killed a large number of farmed shrimp in many Asian countries. It has been confirmed that the culprit is Vibrio parahaemolyticus. The bacteria are transmitted orally and colonize the shrimp digestive tract to produce toxins that cause tiss...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A23K50/80A23K20/147
CPCC07K7/08A61P31/04A23K50/80A23K20/147A61K38/00
Inventor 杨燊王铭遥黄世英郑明静石林凡翁武银
Owner JIMEI UNIV
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