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Method for detecting glucose by using novel enzyme-free glucose sensor

A glucose sensor and glucose technology, applied in the field of electrochemical detection, can solve problems such as high cost, and achieve the effects of easy operation, enhanced performance, and high sensitivity

Active Publication Date: 2021-07-09
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the enzymatic glucose sensor has the advantages of fast response and good selectivity, it is still affected by factors such as high cost, complicated enzyme immobilization process and temperature and pH.

Method used

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  • Method for detecting glucose by using novel enzyme-free glucose sensor
  • Method for detecting glucose by using novel enzyme-free glucose sensor
  • Method for detecting glucose by using novel enzyme-free glucose sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for detecting glucose by a novel enzyme-free glucose sensor, the preparation method of the working electrode of the enzyme-free glucose sensor comprises the following steps:

[0040] Step 1. Precursor preparation

[0041] 1. Ultrasonically mix and disperse 7.5mmol nickel nitrate hexahydrate and 2.5mmol ferric nitrate nonahydrate into 20mL deionized aqueous solution, and record it as solution A;

[0042] 2. Prepare 30 mL of 2.5 mmol sodium hydroxide solution, which is called solution B; add 23 vol.% formamide into 20 mL of aqueous solution containing 0.1 M sodium nitrate, and call it solution C.

[0043] Step 2, the preparation of NiFe-PMLDHs nanosheet material

[0044] Add solutions A and B to solution C at the same time. After the reaction is completed, centrifuge, wash, and store wet to obtain the material NiFe-PMLDHs; in the magnetic stirring of the water bath, slowly drop solutions A and B into solution C at the same time, and the temperature of the oil ba...

Embodiment 2

[0052] Example 2: Sensor response to glucose

[0053] Use the modified electrode prepared in Example 1 as the working electrode, the platinum wire as the counter electrode, and the Ag / AgCl electrode as the reference electrode. One end of these three electrodes is connected to an electrochemical workstation (Autolab), and 0.1M NaOH solution is used as the electrolyte for electrochemical testing. .

[0054] The specific test conditions are:

[0055] The cyclic voltammetry test technology is adopted, and the sensor is used to perform cyclic voltammetry scanning in the potential range of 0V to 0.7V. The cyclic voltammogram comparison of the sensor in the solution of 1mM glucose / 0.1M sodium hydroxide and the solution without glucose ( image 3 ).

[0056] The measurement result is:

[0057] It can be found that after adding 1mM glucose, the oxidation peak current has a significant increase of about 400μA, indicating that the sensor has an obvious electrochemical response to glu...

Embodiment 3

[0065] Example 3: Application of the sensor in the detection of glucose in actual serum samples

[0066] Use the modified electrode made by implementing a modified electrode as a working electrode, platinum wire as a counter electrode, and an Ag / AgCl electrode as a reference electrode. One end of these three electrodes is connected to an electrochemical workstation (Autolab), and 0.1M NaOH solution is used as an electrolyte for electrochemical testing.

[0067] The specific test conditions are:

[0068] Under the applied potential of 0.5V, the current response value of the enzyme-free glucose sensor based on the porous single-layer nickel-iron layered double hydroxide nanosheets to the known concentration of glucose solution was tested, and the 1% human serum sample was detected by the standard sample recovery method. glucose. Prepare three serum samples by adding glucose with known concentrations of 6.0, 7.0, and 8.5mM, perform an amperometric experiment, and detect the curr...

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Abstract

The invention relates to a method for detecting glucose by using a novel enzyme-free glucose sensor, and belongs to the field of electrochemical detection. The porous single-layer ferronickel layered double hydroxides (PM-LDHs) nanosheet material is successfully synthesized through a one-step water bath method, the material is modified on a glassy carbon electrode, and rapid and accurate detection of glucose is achieved. The enzyme-free glucose sensor has the advantages of high sensitivity, good reproducibility, good selectivity, high stability, strong anti-interference capability, wide detection range and the like, the detection range of glucose is 0.01-2.49 mM, the detection limit is 3.2 [mu] M, and the sensitivity is as high as 5179.11 [mu] A[mu]M <-1> cm <-2>. The method for preparing the modified electrode of the sensor is simple, the detection method is high in response speed, steady-state current can be achieved within 1 second, operation is easy and convenient, cost is low, and batch and large-scale production and use can be achieved. The method has a good application prospect in the fields of clinical diagnosis, food industrial analysis and the like.

Description

technical field [0001] The invention relates to a method for detecting glucose by a novel enzyme-free glucose sensor, which belongs to the field of electrochemical detection. Background technique [0002] Over the past few decades, millions of people have suffered from the chronic disease of diabetes, which not only leads to serious health problems, but also leads to heavy medical burden and indirect social costs. Unfortunately, since there is no cure, people with diabetes must keep their disease under control by regularly measuring blood sugar levels. Therefore, a convenient and reliable glucose determination method is needed. [0003] So far, many methods for detecting glucose have been developed, such as spectrophotometry, fluorescence spectrophotometry, colorimetry, and infrared spectroscopy. However, these methods have some disadvantages, such as time-consuming, professional operation, expensive equipment, etc., which limit their clinical application. The electrochem...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/3277G01N27/48
Inventor 袁群惠干为潘旺
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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