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Method for extracting catechin, and application thereof

A technology of catechins and extracts, which is applied in the direction of anti-toxic agents, organic chemistry, anti-viral agents, etc., can solve the problems that it cannot be used to prepare catechin conjugates, the synthesis pathway does not provide distinction, and the synthesis of metabolites is not reported. , to achieve the effects of improving solubility and stability, improving thermal degradation, and improving the uniformity of crushing

Inactive Publication Date: 2021-07-13
北京杰森源生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of "hybrid" technology is the inherent lack of modularity
[0008] In fact, the Rolando method is limited to the semi-synthesis of "simple" catechin conjugates and cannot be used to prepare complex catechin conjugates
Another disadvantage is the low yield of selective protection of catechins on the free catechol ring (i.e. the first step of the semi-synthetic method)
However, several stereoselective syntheses of optically active catechins and derivatives have been reported in the literature, and to the best of our knowledge, no synthesis of the corresponding metabolites (methyl ethers, sulfate esters, or glucuronides) has been reported.
In particular, the synthetic pathway described does not provide for distinguishing the hydroxyl groups of the catechol B ring from those of the phloroglucinol A ring on the catechin backbone, thereby failing to introduce conjugation at specific sites of the catechin in a regio-controlled manner.

Method used

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  • Method for extracting catechin, and application thereof
  • Method for extracting catechin, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for extracting catechins, comprising the steps of:

[0036] (1) The plant tissues of the tea tree are roots, stems and leaves. Grind the plant tissue of the tea tree with a pulverizer until it can pass through a sieve with a pore size of 1.3mm, and then soak in water at room temperature for 3 hours; ultrasonic treatment, 300W ultrasonic treatment at 50°C for 30min. Add compound enzyme solution, enzymolyze at 45°C for 8 hours, then stand still or centrifuge and take the supernatant as the enzymatic hydrolysis product; compound enzymes include cellulase, xylanase, amygdalinase, cellulase, The mass ratio of xylanase and amygdalinase is 4.5:5:1.2, and it is configured into a composite enzyme aqueous solution with a mass concentration of 5%; wherein cellulase is 420U / mg, xylanase is 5U / mg, bitter Amygdalinase is 8U / mg.

[0037] (2) Take the enzymatic hydrolysis product and add quaternary ammonium salt and stabilizer, the stabilizer is 4-picolinic acid, and then he...

Embodiment 2

[0042] A method for extracting catechins, comprising the steps of:

[0043] (1) The plant tissue of the tea tree is one or more of roots, stems and leaves. Grind the plant tissue of the tea tree with a pulverizer until it can pass through a sieve with a pore size of 1.55 mm, and then soak in water at room temperature for 3 hours; ultrasonic treatment at 50 °C for 30 min at 300 W. Add compound enzyme solution, enzymolyze at 65°C for 5 hours, then stand still or centrifuge and take the supernatant as the enzymatic hydrolysis product; compound enzymes include cellulase, xylanase, amygdalinase, cellulase, The mass ratio of xylanase and amygdalinase is 4.5:5:1.2, and it is configured into a composite enzyme aqueous solution with a mass concentration of 5%; wherein cellulase is 420U / mg, xylanase is 5U / mg, bitter Amygdalinase is 8U / mg.

[0044] (2) Take the enzymatic hydrolysis product and add quaternary ammonium salt and stabilizer, the stabilizer is 4-picolinic acid, and then hea...

Embodiment 3

[0049] A method for extracting catechins, comprising the steps of:

[0050] (1) The plant tissue of the tea tree is one or more of roots, stems and leaves. Grind the plant tissue of the tea tree with a pulverizer until it can pass through a sieve with a pore size of 1.55 mm, and then soak in water at room temperature for 2 hours; ultrasonic treatment at 50 °C for 30 min at 300 W. Add compound enzyme solution, enzymolyze at 65°C for 8 hours, then stand still or centrifuge and take the supernatant as the enzymatic hydrolysis product; compound enzymes include cellulase, xylanase, amygdalinase, cellulase, The mass ratio of xylanase and amygdalinase is 4.5:5:1.2, and it is configured into a composite enzyme aqueous solution with a mass concentration of 5%; wherein cellulase is 420U / mg, xylanase is 5U / mg, bitter Amygdalinase is 8U / mg.

[0051] (2) Take the enzymatic hydrolysis product and add quaternary ammonium salt and stabilizer, the stabilizer is 4-picolinic acid, and then hea...

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Abstract

The invention relates to a method for extracting catechin, and application thereof. The method comprises the following steps: grinding plant tissues of tea trees by adopting a grinder, adding water for soaking, carrying out ultrasonic treatment, and adding a compound enzyme solution; adding a solvent into the enzymolysis product for extraction, centrifuging an extract crude product, taking supernatant, adding an extracting agent for extraction, and performing column chromatography separation on the extracted crude liquid to obtain a primary crystal substance; and recrystallizing by using a crystallization liquid to obtain the catechin. The isonicotinic acid is added in the extraction process, so that the stability of the catechin is effectively improved, the oxidation loss of the catechin can be reduced to the greatest extent, and meanwhile, the condition that the catechin is degraded by heating can be improved; and a compound enzyme solution system of cellulase, xylanase and glucosidase is adopted, so that cell walls are damaged more sufficiently, and the resistance of substances in cells to be dissolved out can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of natural plant extraction, and in particular relates to a method for extracting catechins and an application thereof. Background technique [0002] Catechin, also known as tea tannin, and caffeine are two important functional components in tea, accounting for 65% to 80% of the total tea polyphenols. Catechin is one of the polyphenols, also known as Flavanols. Catechin has a positive effect on human health. Catechin includes ester catechin and simple catechin. Its main components are: EGCG (epigallocatechingallate), EGC (epigallocatechin), ECG (epicatechingallate), EC (epicatechin) and catchin. It mainly includes ester-type catechin and non-ester-type catechin. Ester-type catechin is the main active and water-soluble component in tea polyphenols in fresh tea leaves. It is the component with the highest content in catechin, accounting for catechin About 70% of the protein, which has two main components: L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P39/06A61P31/12
CPCC07D311/62A61P39/06A61P31/12
Inventor 张昱
Owner 北京杰森源生物科技有限责任公司
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