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A kind of biological bacterial fertilizer and method for promoting the growth of rhododendron alpine tissue culture seedlings

A technology of biological bacterial fertilizer and tissue culture seedlings is applied in the field of biological bacterial fertilizers for promoting the growth of alpine Rhododendron tissue culture seedlings, which can solve the problems of high control cost, poor effect, and difficulty in promoting production in a large area, and achieves the promotion of root growth and production efficiency. The effect of developing, facilitating root absorption, and promoting absorption

Active Publication Date: 2022-05-17
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current development of the alpine rhododendron industry is not satisfactory. The lack of artificial resource cultivation technology has restricted the high-quality development of the alpine rhododendron industry in China. Among them, how to improve the survival rate of tissue-cultured seedlings and strong seedlings has always been a technical bottleneck.
At present, the following methods are adopted for the survival rate and strong seedlings of alpine rhododendron tissue culture seedlings. One is to optimize the management of light, temperature, water and nutrients in the process of tissue culture and hardening, and artificially construct environmental conditions that are conducive to the growth of tissue culture seedlings. Although this method can improve the survival rate to a certain extent, it is difficult to promote production on a large scale due to the high cost of controlling these conditions. At the same time, it has an unsustainable disadvantage in promoting the growth of seedlings in the later stage; Farmyard manure, etc., can improve the survival rate and strong seedlings of tissue culture seedlings in the form of substrate mixing, root topdressing, and foliar fertilization, but the effect is not good, and there is also a shortcoming that the fertilizer effect cannot be sustained for a long time after fertilization

Method used

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  • A kind of biological bacterial fertilizer and method for promoting the growth of rhododendron alpine tissue culture seedlings
  • A kind of biological bacterial fertilizer and method for promoting the growth of rhododendron alpine tissue culture seedlings
  • A kind of biological bacterial fertilizer and method for promoting the growth of rhododendron alpine tissue culture seedlings

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preparation example Construction

[0028] In parts by volume, the biological bacterial fertilizer for promoting the growth of rhododendron alpine tissue culture seedlings according to the present invention preferably includes 35-60 parts of pumpkin juice, more preferably 40-55 parts, and even more preferably 50 parts. In the present invention, the pumpkin juice is preferably prepared from pumpkin powder or fresh pumpkin. When fresh pumpkin is used as raw material to prepare pumpkin juice, the preparation method of the pumpkin juice preferably includes the following steps: crushing the pumpkin pulp to obtain crushed pumpkin pulp; mixing the crushed pumpkin pulp with water, steaming over water, Get pumpkin juice. In the present invention, the mass ratio of crushed pumpkin pulp to water is preferably 100g:(300-400)mL, more preferably 100g:350mL. In the present invention, the cooking time is preferably 10-15 minutes, more preferably 15 minutes. When pumpkin juice is prepared from pumpkin powder, the concentration...

Embodiment 1

[0033] A biological bacterial fertilizer for promoting the growth of rhododendron alpine tissue culture seedlings, which is composed of the following components by volume: 11 parts of bacterial liquid of Saccharomyces cerevisiae, 8 parts of bacterial liquid of Trichoderma longibrachiae, and bacterial liquid of Paecilomyces lilacinus 11 parts, 20 parts of bacterial liquid of Bacillus polymyxa and 50 parts of pumpkin juice;

[0034] The viable count of the bacterium liquid of described Saccharomyces cerevisiae is 7 * 10 7 CFU / mL; The viable count of the bacterium liquid of described Trichoderma longibrachiae is 5 * 10 7 CFU / mL; The number of live bacteria of the bacterium liquid of described Paecilomyces lilacinus is 6 * 10 7 CFU / mL; the viable count of the bacterium liquid of described bacillus polymyxa is 6 * 10 8 CFU / mL.

[0035] The preparation method is as follows:

[0036] 1) Prepare sterile water, put distilled water in a sterile wide-mouth bottle, put it in a pressur...

Embodiment 2

[0042] A biological bacterial fertilizer for promoting the growth of rhododendron alpine tissue culture seedlings, which is composed of the following components by volume: 10 parts of bacterial liquid of Saccharomyces cerevisiae, 9 parts of bacterial liquid of Trichoderma longibranchus, and bacterial liquid of Paecilomyces lilacinus 11 parts, 22 parts of Bacillus polymyxa bacteria liquid and 48 parts of pumpkin juice;

[0043] The viable count of the bacterium liquid of described Saccharomyces cerevisiae is 7 * 10 7 CFU / mL; The viable count of the bacterium liquid of described Trichoderma longibrachiae is 7 * 10 7 CFU / mL; the number of live bacteria in the bacterium solution of Paecilomyces lilacinus is 8×10 7 CFU / mL; the viable count of the bacterium liquid of described bacillus polymyxa is 6 * 10 8 CFU / mL.

[0044] The preparation method is the same as in Example 1.

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Abstract

The invention provides a biological bacterial fertilizer and a method for promoting the growth of rhododendron alpine tissue culture seedlings, belonging to the technical field of biological bacterial fertilizer. The biological bacterial fertilizer for promoting the growth of rhododendron alpine tissue culture seedlings provided by the invention comprises Saccharomyces cerevisiae, Trichoderma longibranchus, Paecilomyces lilacinus, Bacillus polymyxa and squash. The present invention prepares the biological bacterial fertilizer that can significantly improve the survival rate and seedling strengthening effect of Rhododendron alpine tissue culture seedlings after transplanting through the reasonable compounding of microbial agents and the screening of suitable nutrients, and promotes the development of Rhododendron alpine in China. High-quality development of the industry.

Description

technical field [0001] The invention belongs to the technical field of biological bacterial fertilizer, and in particular relates to a biological bacterial fertilizer and a method for promoting the growth of rhododendron alpine tissue culture seedlings. Background technique [0002] Rhododendron is known as "the beauty of flowers" and occupies an important position in the flower market all over the world. The alpine rhododendron has become a boutique among rhododendrons because of its bright colors, rich colors, and huge flowers. China is a country with large rhododendron resources, and the distribution of alpine rhododendron resources is large and concentrated. However, the current development of the alpine rhododendron industry is not satisfactory. The lack of artificial resource cultivation technology has restricted the high-quality development of the alpine rhododendron industry in China. Among them, how to improve the survival rate of tissue-cultured seedlings and stro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/60C12N1/16C12N1/14C12N1/20C12R1/865C12R1/885C12R1/79C12R1/12A01C21/00
CPCC05G3/00C05G3/60C12N1/16C12N1/14C12N1/20A01C21/005C12N1/18C12R2001/865C12R2001/885C12R2001/12C12R2001/79C05F11/08A01P21/00A01N63/32A01N63/38A01N63/22A01N63/20
Inventor 龚记熠刘杰李菲唐婧唐明张习敏乙引
Owner GUIZHOU NORMAL UNIVERSITY
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