Magnetic nano material with cell membrane reversely coated with cell membrane as well as preparation method and application of magnetic nano material
A magnetic nanometer and cell membrane technology, applied in the field of bionic materials, can solve the problem of uncontrollable orientation of cell membrane and membrane receptors, and achieve the effect of achieving consistency, high specific surface area, and improving analysis efficiency
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Embodiment 1
[0044] Specifically, the preparation method is divided into two parts:
[0045] 1. SA-Fe 3 o 4 Synthesis
[0046] Synthesis of Fe by solvothermal method 3 o 4 magnetic nanoparticles. The specific operation is briefly as follows: under ultrasonic conditions FeCl 3 ·H 2 O (3.60 g) and trisodium citrate (0.72 g) were dissolved in ethylene glycol (100 mL), then NaAc (4.80 g) was added and after vigorous stirring at 50 °C for 30 min, the mixture was then sealed in a Teflon liner with a capacity of 100 mL stainless steel autoclave. After the stainless steel autoclave was kept at 200°C for 10 hours, the resulting product was washed six times with methanol and water to obtain a neutral product, namely Fe 3 o 4 magnetic nanoparticles.
[0047] Synthetic Fe 3 o 4 @SiO 2 Nanoparticles, the details of preparation are described as follows: 0.1g Fe 3 o 4 The magnetic nanoparticles were uniformly dispersed in a mixed solution of ethanol (80mL) and ultrapure water (12mL) after ...
Embodiment 2
[0059] Embodiment 2 adsorption test
[0060] For the prepared IOCMMNPs, the present invention has carried out the investigation and research on the adsorption property. The result is as Figure 4 As shown in A, as the concentration of gefitinib gradually increased, the adsorption capacity of IOCMMNPs for gefitinib increased rapidly at the beginning, then slowed down, and then the adsorption capacity became stable and reached saturation. The final saturated adsorption capacity is 302 mg g -1 . In addition, the adsorption capacity of IOCMMNPs for gefitinib was obviously much higher than that of MNPs for gefitinib. The resulting data were then further processed by Freundlich, Langmuir, Scatchard and Dubinin-Radushkevich isotherms, respectively, to assess the binding properties of IOCMMNPs; see Figure 4 In B, it can be clearly observed that the Freundlich isotherm (FI) model can describe the static adsorption well and has the highest correlation coefficient (for α 1A / MNGs, ...
Embodiment 3
[0065] Embodiment 3 screening performance test
[0066] The magnetic biomaterial invented in this experiment aims to establish a controllable cell membrane magnetic solid-phase extraction drug screening model for intramembrane receptors, so as to provide a fast, simple, targeted and effective sample pretreatment technology, which can be used to extract complex quickly find and screen target compounds. In order to determine the applicability of the proposed material, the extract of the traditional Chinese medicine Nuxe chinensis was extracted using IOCMMNPs, and the results are shown in Figure 6 middle. From Figure 6 In (a), (b) and (c), it can be seen that compared with the chromatographic peaks of the traditional Chinese medicine Nuxychnium extract, a large number of components were washed out during the screening process, and finally the main components of the two Nuxychnium points were screened out by IOCMMNPs. To identify the two screened components, further analysis...
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