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Agricultural humic acid nutritional agent with biological control function as well as preparation method and application of agricultural humic acid nutritional agent

A humic acid and nutrient technology, applied in the field of microorganisms, can solve the problems of low spore production, poor growth, low insecticidal toxicity, etc., and achieve the effect of promoting crop growth, low equipment requirements, and simple operation

Inactive Publication Date: 2021-08-03
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of agricultural humic acid nutrient with biological control function and its preparation method and application, which solves the poor growth and spore production of existing Beauveria bassiana products. The problem of less insecticidal toxicity and low insecticidal toxicity, at the same time, the nutrient can provide all-round nutrition for crops and improve crop resistance to diseases and insects

Method used

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  • Agricultural humic acid nutritional agent with biological control function as well as preparation method and application of agricultural humic acid nutritional agent
  • Agricultural humic acid nutritional agent with biological control function as well as preparation method and application of agricultural humic acid nutritional agent
  • Agricultural humic acid nutritional agent with biological control function as well as preparation method and application of agricultural humic acid nutritional agent

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preparation example Construction

[0034] A preparation method of an agricultural humic acid nutrient with a biological control function, comprising the following steps: cultivating Beauveria bassiana in a liquid medium for liquid fermentation culture, then mixing the fermented liquid obtained from the liquid fermentation culture with the solidified culture liquid, mixing The volume ratio is 3: (1 ~ 2). When mixing, use a mixer to stir. After mixing evenly, let it stand until it solidifies to carry out solid fermentation. After the solid is cultivated for a period of time, stop the solid fermentation to obtain a fermentation intermediate; put the fermentation intermediate in Naturally air-dried at 22-28°C, and then crushed to a size of 60-80 mesh to obtain nutrient granules, which is an agricultural humic acid nutrient with biological control function according to the present invention.

[0035] The raw materials of the fermentation medium used in the fermentation are: the liquid fermentation medium is composed ...

Embodiment 1

[0044] The fermentation medium used in the fermentation is respectively: the liquid fermentation medium is composed of the following raw materials in weight percentage: chitosan 1.0%, yeast powder 0.5%, potassium humate 3.0%, magnesium sulfate 0.5%, calcium chloride 1.5% %, distilled water 1L, surfactant 0.5%. The solidified culture solution is composed of the following raw materials in weight percentage: 3% sodium alginate, 1.0% starch, 0.5% glycerin, and 1 L of distilled water.

[0045] Cultivating the Beauveria bassiana in a liquid medium is Beauveria bassiana, the surfactant is span20, and then the fermented liquid cultured in the liquid fermentation medium is mixed with the solid culture liquid in a mixing ratio of 3:2, and stirred with a mixer, After mixing evenly, let it stand for solid fermentation, cultivate it on a solid medium for a period of time, stop fermentation, and obtain a fermentation intermediate; naturally air-dry the fermentation intermediate at a tempera...

Embodiment 2

[0049] The fermentation medium that described fermentation adopts is respectively: liquid fermentation medium is made up of the raw material of following percentage by weight: chitosan 1.5%, yeast powder 0.5%, potassium humate 2.5%, magnesium sulfate 1.0%, calcium chloride 1.5% %, distilled water 1L, surfactant 1.0%. The solidified culture solution is composed of the following raw materials in weight percentage: 3% sodium alginate, 0.5% starch, 1.0% glycerin, and 1 L of distilled water.

[0050] Cultivating the Beauveria bassiana in a liquid medium is Beauveria bassiana, the surfactant is span60, and then the fermentation liquid obtained from the cultivation of the liquid fermentation medium is mixed with the solidified culture liquid in a mixing ratio of 3:2, and stirred with a mixer, After mixing evenly, let it stand for solid fermentation. When the solid medium reaches 24 hours, stop the fermentation to obtain the fermentation intermediate; air-dry the fermentation intermed...

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Abstract

The invention discloses an agricultural humic acid nutritional agent with a biological control function as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: fermenting beauveria in a compound culture medium containing humic acid to obtain a fermentation intermediate; subjecting the obtained fermentation intermediate to air drying treatment and then conducting smashing, so as to obtain the agricultural humic acid nutritional agent with the biological control function. The Beauveria is Beauveria bassiana or Beauveria brongniartii; and the compound culture medium comprises a liquid culture medium and a solidified culture solution. The preparation method is easy to operate, low in equipment requirement and environmentally friendly, comprehensive nutrition can be provided for crops, the disease resistance of the crops is improved, and development and fruiting of fruit trees are promoted; and the agricultural humic acid nutritional agent has the advantages of strong adhesion, wide application range and good control effect, and can be applied to pest control of budworms.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to an agricultural humic acid nutrient with biological control function and its preparation method and application. Background technique [0002] The small peach borer belongs to the order Lepidoptera. It has the characteristics of nocturnal and backlight. It is one of the largest and most harmful pests in fruit tree production. It mainly eats fruit in the larval stage. For a long time, chemical control has been an effective means to control borer-eating insects, but the continuous use of chemical insecticides will cause a series of problems such as drug resistance and food and environmental safety. And because of its nocturnal and backlight habits, chemical pesticides have disadvantages such as difficulty in spraying at night and poor hit rate. Therefore, traditional pesticide control is difficult, ineffective, and easy to cause environmental pollution. It has gradually failed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05G3/80C12N1/14C12R1/645
CPCC05G3/60C05G3/80C12N1/14C05F11/02C05F11/08
Inventor 宋洁韩家旋牛育华李龙陈莉君
Owner SHAANXI UNIV OF SCI & TECH
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