Method for rapidly obtaining konjak seedlings

Abstract

The invention discloses a method for rapidly obtaining konjak seedlings. The method comprises the steps of konjak embryogenic callus induction, konjak micro-corm induction, konjak micro-corm proliferation, konjak artificial seed preparation and konjak clustered seedling differentiation. According to the method, a plant tissue culture technology and an artificial seed preparation technology are combined, the konjac micro-corm is used as an artificial seed matrix, the problems of soft rot, variety degeneration and the like in traditional konjac planting are solved, a large number of clustered seedlings with a good detoxification effect can be obtained within a short time, and compared with konjak seedling seeds, the konjak artificial seeds are convenient to store and transport, high in breeding efficiency and capable of meeting market requirements and have high popularization value.

Description

technical field [0001] The invention relates to the technical field of rapid propagation of medicinal and edible plants, in particular to a method for rapid propagation of konjac, and in particular to a method for preparing artificial seeds of konjac. Background technique [0002] Konjac, also known as konjac, is a perennial herbaceous plant of the genus Amorphophallus Blume of the family Araceae. Its economic organ is the underground corm, which mainly contains glucomannan (KGM), starch, alkaloids, vitamins, minerals, and also contains a small amount of protein, various amino acids, ceramides, flavonoids and other nutrients. Konjac has a variety of pharmacological effects, which can be summarized as weight loss, fat lowering, blood sugar lowering, anti-cancer, anti-aging, and improving intestinal microenvironment. It has been listed as one of the top ten health foods by the United Nations Health Organization. Moreover, konjac is the only major economic crop that can provid...

AI Technical Summary

Problems solved by technology

Traditional konjac planting methods are limited by seasons and external environmental conditions, and there are problems such as slow propagation speed and long growth cycle. With the extension of planting years, there will be problems such as variety degradation, soft rot damage and continuous sitting obstacle damage. As a result, the planting area of ​​konjac has dropped sharply and the quality of the germplasm has declined, and the output of konjac fine powder is in short supply. Therefore, the konjac industry urgently needs improved breeding

Conventional konjac planting uses konjac bulbs as propagation seeds, and requires 4,000 konjac bulbs per mu, and the cost of planting seeds is very high; since konjac bulbs belong to vegetative bodies, their reproductive form belongs to vegetative reproduction, and the virus will slowly accumulate after years of seeding After reaching a certain level, it will cause slow growth and a large drop in yield; moreover, the traditional storage of bulbs needs to take up a lot of space, and there are outstanding problems such as long dormancy periods.

[0004]Konjac The traditional planting method of konjac is to use bulbs as seeds, which will be limited by seasons and external environmental conditions, with slow reproduction speed and long growth cycle. There are outstanding problems such as a large amount of reserved seeds and high costs; and with the extension of the planting period, a series of problems such as the accumulation of seed viruses accumulated year after year, there have been problems such as variety degradation, soft rot hazards, and increased hazards of continuous sitting obstacles, resulting in a sharp decline in the planting area of ​​konjac and The quality of germplasm is declining, and the output of konjac fine powder is in short supply; moreover, the traditional bulb block storage needs to occupy a lot of space, the quality of the bulb block decreases during storage, and there are physiological problems such as dormancy

[0005] At present, research on konjac tissue culture is limited to using explants such as konjac leaves, petioles, tubers to induce callus-differentiate into seedlings-harden seedlings-transplant, In the process of callus differentiation into seedlings, the callus is easy to brown and needs interphase transfer, and this process is limited to the laboratory research stage, and industrial production cannot be realized; the cost of tissue culture seedling preparation is high, and the degree of automation of production is low , the survival rate of transplanted test tube seedlings is low and it is inconvenient to transport and store; the cultivation of tissue culture seedlings requires a lot of space, etc.