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A functional peptide mixed powder for promoting the proliferation of osteogenic precursor cells and its application

A technology of osteogenic precursor cells and functional peptides, which is applied in the functions of food ingredients, bone/connective tissue cells, and applications, can solve the problems of waste of functional peptides, poor proliferation ability of osteogenic precursor cells, and avoid waste. , improve the stability and its nutritional value, and promote the effect of intestinal absorption

Active Publication Date: 2022-06-17
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem of poor ability of the existing extracted peptides to promote the proliferation of osteoblast precursor cells, and to provide a functional peptide mixed powder for promoting the proliferation of osteoblast precursor cells and its application. The surface of the functional peptide extracted from tuna bone Cover the protective layer, control the release position and release speed of functional peptides, improve the stability and nutritional value of functional peptides, promote the absorption of human intestinal tract, prepare functional peptide products that are easy to digest and can be fully absorbed, and avoid functional peptides. Peptide waste

Method used

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  • A functional peptide mixed powder for promoting the proliferation of osteogenic precursor cells and its application

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preparation example Construction

[0034] 3. The preparation process of the functional peptide microcapsules is:

[0035] (1) Dissolve cellulose acetate in ethanol, add starch and mix evenly, add diglycerol dropwise, continue to add fibroin fiber and dibutyltin dilaurate, heat up to 50-55°C for reaction, and add functional peptide after the reaction is complete powder, and continue to stir for 20-25min, and homogenize for 5-10min under the condition of 2000-2300r / min; the obtained solution is under the condition of inlet air temperature 45-50°C, outlet air temperature 30-35°C, feeding speed 70-90mL / h Spray drying is carried out to obtain single-layer coated microcapsules; the ratio of cellulose acetate, ethanol, starch, diglycerol, silk protein fiber, dibutyltin dilaurate and functional peptide powder is 20-22g: 450-480mL: 15-18g: 40-45mL: 12-16g: 0.5-0.8g: 15-20g; the length of the fibroin fiber is 1-1.5mm.

[0036] (2) Mix the components of the wall material according to the proportion, add the above-mention...

Embodiment 1

[0038] 1. Application of functional peptide mixed powder to promote the proliferation of osteogenic precursor cells in beverages. The preparation process of the beverage: adding functional peptide mixed powder with a mass fraction of 3.5% in warm water at 48° C., stirring evenly to completely dissolve it, and obtaining a functional peptide beverage.

[0039] 2. The formula of the functional peptide mixed powder includes: 35% functional peptide microcapsules; 15% galactooligosaccharide; 8% casein phosphopeptide; 8% taurine; 8% vitamin C; 1.5% microcrystalline cellulose and 1.5% carboxymethylcellulose; the remainder is whey protein powder.

[0040] 3. The preparation process of the functional peptide microcapsules is:

[0041] (1) Dissolve cellulose acetate in ethanol, add starch and mix evenly, add diglycerol dropwise, continue to add fibroin fiber and dibutyltin dilaurate, heat up to 53°C for reaction, add functional peptide powder after the reaction is complete, And continu...

Embodiment 2

[0044] 1. Application of functional peptide mixed powder to promote the proliferation of osteogenic precursor cells in beverages. The preparation process of the beverage: adding a functional peptide mixed powder with a mass fraction of 2% in warm water at 40° C., stirring evenly to completely dissolve it, and obtaining a functional peptide beverage.

[0045] 2. The formula of the functional peptide mixed powder includes: 30% functional peptide microcapsules; 20% galactooligosaccharide; 5% casein phosphopeptide; 10% taurine; 5% vitamin C; 2% microcrystalline cellulose and 1% carboxymethyl cellulose; the remainder is whey protein powder.

[0046] 3. The preparation process of the functional peptide microcapsules is:

[0047] (1) Dissolve cellulose acetate in ethanol, add starch and mix evenly, add diglycerol dropwise, continue to add fibroin fiber and dibutyltin dilaurate, heat up to 50°C for reaction, add functional peptide powder after the reaction is complete, And continue ...

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Abstract

The present invention relates to the technical field of peptide extraction from fish bones. Aiming at the problem of poor ability of the extracted peptides in the prior art to promote the proliferation of osteogenic precursor cells, a functional peptide mixed powder for promoting the proliferation of osteogenic precursor cells and its application are disclosed. The formula of the functional peptide mixed powder includes: 30~40% functional peptide microcapsules; 10~20% galactooligosaccharide; 5~10% casein phosphopeptide; 5~10% taurine; 5~10% vitamin C ; 1~2% microcrystalline cellulose and 1~2% carboxymethyl cellulose; the rest is whey protein powder. By covering the surface of the functional peptide extracted from tuna bone with a protective layer, and by controlling the release position and speed of the functional peptide, the stability and nutritional value of the functional peptide can be improved, and the absorption of the human intestinal tract can be promoted. The functional peptide product that can be fully absorbed avoids the waste of functional peptides and reduces the cost of preparing functional peptides.

Description

technical field [0001] The invention relates to the technical field of peptides extracted from fish bones, in particular to a functional peptide mixed powder for promoting the proliferation of osteogenic precursor cells and its application. Background technique [0002] Osteoporosis is a systemic bone disease that is prone to fracture due to the decrease of bone density and bone quality due to various reasons, the destruction of bone microstructure, and the increase of bone fragility. With the increasing aging of society, the incidence of osteoporosis has jumped to the seventh place among various common diseases in the world, and it is recognized as a serious social public health problem. The incidence of osteoporosis is related to various factors such as living habits, hormone regulation and heredity, and is the result of the combined action of multiple factors. At present, most of the researchers find the factors that cause the balance of bone resorption and bone formatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654A23L2/52A23L33/18A23L33/21A23L33/175A23L33/15A23L33/19A23L29/30A23L29/238A23L29/27A23L29/035A23L29/045A23L29/04A23P10/30C12N2501/998C12N2500/33C12N2500/34A23V2002/00A23V2200/306A23V2250/55A23V2250/284A23V2250/0644A23V2250/708A23V2250/51084A23V2250/51082A23V2250/54252A23V2250/51088A23V2250/5118A23V2250/542A23V2250/506A23V2250/5086A23V2250/5114A23V2250/211A23V2250/628A23V2300/26A23V2300/10
Inventor 肖金星郑刚周宇芳杨志坚赵小立王开扬周小敏劳敏军
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV