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Method for promoting gonad development and artificially inducing spawning of parent fish of diodon holocanthus

A technology of artificial induction of labor and gonadotropin, applied in fish farming, climate change adaptation, animal husbandry, etc., to achieve good palatability, avoid water pollution, and high nutritional content

Active Publication Date: 2021-08-31
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for promoting gonad development and artificially inducing spawning of parent fish of diodon holocanthus
  • Method for promoting gonad development and artificially inducing spawning of parent fish of diodon holocanthus
  • Method for promoting gonad development and artificially inducing spawning of parent fish of diodon holocanthus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 6

[0043] Example 1 Comparison of nutrient enhancement methods in six-spotted acupuncture

[0044] (1) Picking the fish

[0045] In the six-year thorn, the quality of the body has been stabilized, the quality is more than 1000g, the body length is 18cm, the body surface is no injury, the healthy and good healthy and good quality hex spots ( figure 1 ). The selected fish is divided into two groups of the experimental group and the control group, put in a pool in diameter of 5m, water depth 1.2m, stocking density is 5-7 tail / m 3 .

[0046] Cultivate environmental conditions: Salinity 28-32, maintain water temperature through the constant temperature system, from January to February 24-26 ° C, from March to September 26-28 ° C, pH 7.2 ~ 8.5, oxygen remained> 5mg / L, water Layer surface light 500LUX. Drain 30% from 16 pm every day, and clean up the bottom of the pool. After cleaning, add the water temperature of 24-26 ° C, filled with trace water.

[0047] (2) Fish fish strengthen

[...

Embodiment 2 6

[0055] Embodiment 2 Comparison of comparison of six-spot puncture oxytysia

[0056] The abdomen ridge, reproductive hole micro-red and protruding from the experimental group of Example 1 were selected, and the inspection was performed on the operation platform, and the hand was pushed from the fishing belly to the reproductive hole, which can flow out of the white semen or transparent lesiformity. It is determined that it has reached sexual maturity, divided into two groups, and placed in the pool. A set of oxytysis methods for use in the present invention were oxygenated; the other group made it naturally eggs as a control group.

[0057] Umbrella drug use to promote oceanin 2 + velvet gonadotropin I, 5 micrograms of fish weight per kilogram of fish, 350 unit dose injection of 350 units per kilogram of fish weight, oxythate time at 10: 00 ~ 11: 00, the effect time is 40 ~ 46h. After the effect time, the fertilized egg is collected from the affiliates and check the fertilization a...

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Abstract

The invention provides a method for promoting gonad development and artificially inducing spawning of parent fish of diodon holocanthus. The method is suitable for breeding the parent fish of the diodon holocanthus under artificial breeding environment conditions, a bait formula for nutrition enrichment in the breeding process comprises the following components: fresh and live shrimps, live charybdis japonica, squids and fresh and live razor clams, in addition, a breeding enhancer, fish oil powder, vitamin C, vitamin E and taurine are added into the fresh and live shrimps or the fresh and live razor clams according to a certain proportion, and stuffed feeding with the fresh and live shrimps or the fresh and live razor clams is carried out at regular time every day. After 35 days of intensive cultivation, the parent fish is ripened, wherein chorionic gonadotropin and luteinizing hormone releasing hormone A2 are selected as ripening drugs; and after the gonad develops to be mature, spawning induction is conducted through ovulation induction hormone 2 and chorionic gonadotropin I, after the parent fish reaches the spawning induction effect time, sperm discharge, spawning and fertilization are conducted automatically, and then high-quality fertilized eggs are collected and selected for fry breeding. By adopting the method, the synchronization rate of egg development in the gonad of the diodon holocanthus can be effectively increased, the group synchronization rate reaches 80% or above, and the egg diameter is remarkably increased by 17% due to egg laying.

Description

Technical field [0001] The present invention belongs to the parent fish and promote production technical field, particularly, to a barbed six spots Puffer broodstock and artificial oxytocin gonadotropin development method. Background technique [0002] Six plaques barbed puffer (Diodon holocanthus) is actinopterygii outline, tetraodontiformes A Porcupinefish, the gas common name melon Tsai, porcupine like. Tropical oceans of the underlying small fish, the main habitat in the shallow water reef areas, the soft bottom of the sea or open waters. The general line of solitary life, sometimes clustered, the rows juvenile oceanic drift sex life. Six spotted puffer thorn fresh meat, fish skin collagen protein-rich rich, by the market, the highest up to 200-240 yuan / kg, the market has great potential. Currently six spots all sources of barbed puffer sea fishing resources along with the fishermen overfishing or environmental degradation was dropped into a trend. [0003] Six spotted puff...

Claims

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Application Information

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IPC IPC(8): A01K61/10A23K50/80A23K10/22A23K20/142A23K20/158A23K20/174
CPCA01K61/10A23K50/80A23K10/22A23K20/142A23K20/158A23K20/174Y02A40/81
Inventor 岳彦峰施兆鸿蒋科技彭士明苏志星
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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