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Double-sided scaffold and preparation method and application thereof

A double-sided, composite fiber technology, applied in the field of biomaterials, stem cells, and tissue repair research, can solve the problems of degradable polymer materials such as lack of cell recognition signals, mechanical properties and easy degradation to limit applications, unfavorable cell recognition and adhesion, etc. Achieving good biocompatibility, avoiding incomplete periodontal regeneration, and good migration ability

Active Publication Date: 2021-09-03
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical and chemical properties (porosity, mechanical properties, etc.) of the decellularized tissue matrix are poor in modifiability; degradable polymer materials lack cell recognition signals, which are not conducive to cell recognition and adhesion, and the acidic substances formed during degradation will cause surrounding Inflammation; Although natural polymer materials have good cytocompatibility, their mechanical properties and easy degradation limit their application in periodontal regeneration

Method used

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  • Double-sided scaffold and preparation method and application thereof
  • Double-sided scaffold and preparation method and application thereof
  • Double-sided scaffold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] This embodiment provides a double-sided bracket, which is prepared by the following preparation method:

[0106] (1) configure the chitosan collagen spinning solution containing RGD: 3wt% chitosan (M W 3.1×10 5 ~3.75×10 5 Da), 3wt% collagen (M W 1.2×10 6 Da), 0.50wt% polyethylene oxide (M w 9.5×10 4 Da), 0.02mg / mL RGD (Arg-Gly-Asp) and 90wt% aqueous acetic acid were mixed to obtain a spinning solution;

[0107] Add the spinning solution into a 10mL needle tube, use a 23-gauge needle, at an extrusion rate of 0.5cm / min, carry out electrospinning under the conditions of low pressure -2kV and high voltage 20kV, and receive the composite fiber obtained by spinning with aluminum foil ; and then washed three times with ethanol and three times with deionized water; finally dried at -80°C for 18 hours to obtain the chitosan collagen composite fiber layer;

[0108] (2) Take 2g of chitosan collagen composite fiber layer and place it in 5mL of 1mg / mL hydroxyapatite depositi...

Embodiment 2

[0115] This embodiment provides a double-sided stent, and the only difference from Embodiment 1 is that in step (1), the content of polyethylene oxide in the spinning solution is 0.25 wt%, and the content of other components and the preparation steps are the same. example 1.

[0116] Figure 5 For the scanning electron micrograph of the chitosan collagen composite fiber that embodiment 2 provides, as Figure 5 As shown, the PEO content is less, the fiber diameter is lower, but the uniformity of the fiber is better.

Embodiment 3

[0118] This embodiment provides a double-sided stent, and the only difference from Embodiment 1 is that in step (1), the content of polyethylene oxide in the spinning solution is 0.75wt%, and the content of other components and the preparation steps are the same. example 1.

[0119] Figure 6 For the scanning electron micrograph of the chitosan collagen composite fiber that embodiment 3 provides, as Figure 6 As shown, the higher the PEO content, the higher the fiber diameter, but the uniformity of the fiber has decreased.

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Abstract

The invention provides a double-sided scaffold and a preparation method and application thereof. The double-sided scaffold comprises a chitosan collagen composite fiber layer and a hydroxyapatite-modified chitosan collagen composite fiber layer. The scaffold material has a double-layer structure, and the chitosan collagen composite fiber layer is a barrier layer and maintains a space for tissue regeneration; the hydroxyapatite-modified chitosan collagen composite fiber layer is a functional layer and provides active substances for tissue regeneration; and stem cells are combined and adhered to the functional layer to provide a good microenvironment for tissue regeneration, thereby achieving a good repair effect. In addition, the material has good biocompatibility and the ability to promote cell proliferation and differentiation. The double-sided scaffold material is combined with stem cells for tissue regeneration, which is beneficial to the retention of exogenous stem cells at the damaged part, and provides a good tissue regeneration microenvironment for endogenous stem cells and exogenous stem cells, and has guiding significance for the clinical application of tissue regeneration.

Description

technical field [0001] The present invention relates to the technical field of biological materials and stem cells, in particular to a double-sided scaffold and its preparation method and application, in particular to an implantable cell-carrying scaffold material in the field of tissue repair research and its preparation method and application. Background technique [0002] Periodontal tissue is composed of gingiva, periodontal ligament, cementum, and alveolar bone. It is a well-structured tissue whose main function is to provide physical and mechanical support for teeth. Severe periodontitis is an inflammation triggered by oral bacterial biofilms that can lead to severe damage to the soft and hard tissues of the periodontal tissue, impairing tooth function and aesthetics. Although, current treatments can limit disease progression by controlling the inflammatory aspect, complete periodontal regeneration is unpredictable. The ultimate goal of periodontal therapy is to regen...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/04A61L31/02A61L31/00
CPCA61L31/129A61L31/028A61L31/046A61L31/047A61L31/005A61L2300/412C08L5/08C08L89/00
Inventor 胥伟华陈春英闫娜
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA