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Method for purifying lactic acid through aqueous two-phase extraction

An aqueous two-phase and extraction technology, applied in the biological field, can solve the problems of high impurity content in lactic acid fermentation broth, difficulty in separation, and difficulty in meeting polymerization requirements.

Pending Publication Date: 2021-09-03
WANHUA CHEM (SICHUAN) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the deficiencies in the lactic acid purification method in the prior art, and mainly solves the technical problem that the existing lactic acid fermentation liquid has a large amount of impurities, which is difficult to meet the requirements of polymerization and difficult to separate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (1) Extraction of lactic acid fermentation broth

[0079] After the lactic acid fermentation process was finished, the fermented liquid was collected, and its lactic acid concentration was detected to be 18 wt%. Impurities: glucose 0.5%, cation 2000ppm, anion 1800ppm, protein 1%, heteroacid 4600ppm, chromaticity>1000Hazen.

[0080] Bacteria were removed by plate and frame filtration. At 85°C, add 98% concentrated sulfuric acid for acidification. After the pH reaches 1.5, stop adding sulfuric acid to obtain a solid-liquid mixed system containing lactic acid and calcium sulfate precipitates. Plate and frame filtration was performed to remove solid calcium sulfate to obtain a fermentation supernatant. Wherein the concentration of lactic acid is 17.5wt%. Impurities: glucose 0.5%, cation 2200ppm, anion 3400ppm, protein 1%, heteroacid 2400ppm, chromaticity>1000Hazen.

[0081] Add 20% polyvinylpyrrolidone (PVP) of fermentation broth quality to the fermentation supernatant,...

Embodiment 2

[0092] (1) Extraction of lactic acid fermentation broth

[0093] After the lactic acid fermentation process was finished, the fermented liquid was collected, and its lactic acid concentration was detected to be 14wt%. Impurities: glucose 0.1%, cation 2000ppm, anion 3000ppm, protein 3%, heteroacid 4000ppm, chromaticity>1000Hazen

[0094] Bacteria were removed by plate and frame filtration. At 70°C, add 98% concentrated sulfuric acid for acidification. After the pH reaches 2.0, stop adding sulfuric acid to obtain a solid-liquid mixed system containing lactic acid and calcium sulfate precipitates. Plate and frame filtration was performed to remove solid calcium sulfate to obtain a fermentation supernatant. Wherein the concentration of lactic acid is 14wt%. Impurities: glucose 0.1%, cation 2000ppm, anion 3300ppm, protein 3%, heteroacid 6000ppm, chromaticity>1000Hazen.

[0095] Add 30% polyvinylpyrrolidone (PVP) of fermentation broth quality in the fermentation supernatant, 18%...

Embodiment 3

[0106] (1) Extraction of lactic acid fermentation broth

[0107] After the lactic acid fermentation process was finished, the fermented liquid was collected, and its lactic acid concentration was detected to be 15 wt%. Impurities: glucose 1.6%, cation 1500ppm, anion 1800ppm, protein 2.8%, heteroacid 5000ppm, chromaticity>1000Hazen.

[0108] Bacteria were removed by plate and frame filtration. At 60°C, add 98% concentrated sulfuric acid for acidification. After the pH reaches 2.5, stop adding sulfuric acid to obtain a solid-liquid mixed system containing lactic acid and calcium sulfate precipitates. Plate and frame filtration was performed to remove solid calcium sulfate to obtain a fermentation supernatant. Wherein the concentration of lactic acid is 14.7wt%. Impurities 1.6%, cations 2600ppm, anions 2400ppm, protein 2.8%, heteroacids 4100ppm, chromaticity>1000Hazen.

[0109] Add 30% polyvinylpyrrolidone (PVP) and 10% quaternized polyacrylamide to the fermentation supernata...

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PUM

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Abstract

The invention discloses a method for purifying lactic acid through aqueous two-phase extraction. The aqueous two-phase extraction system is formed by adding lower alcohol or lactate and a water-soluble polymer material into lactic acid fermentation liquor; the aqueous two-phase extraction system is adopted for extracting lactic acid fermentation liquor, after standing and layering, an extract phase containing lactic acid, low alcohol or lactate and water is obtained on the upper layer, a raffinate phase containing impurities, a water-soluble polymer material and water is obtained on the lower layer, and therefore separation of the lactic acid and the impurities is achieved. The method is simple to operate, low in material consumption, high in lactic acid yield and free of side reaction, and can be used for industrial production of high-purity lactic acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to a method for preparing high-purity lactic acid by two-phase extraction. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, is one of the three major organic acids in nature. As an important raw material, it is widely used in the fields of food, medicine, chemical industry and materials. The most important application is to prepare A monomer of biodegradable material polylactic acid. At present, the mainstream preparation method of lactic acid is the fermentation method, which uses the metabolism of microorganisms to convert glucose or starch raw materials into lactic acid. Compared with chemical methods, it has the advantages of low raw material cost, environmental protection, and low pollution. However, microbial fermentation needs to add nutrients such as yeast powder and inorganic salts, and the metabolites in the process are relatively complex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/48C07C51/09C07C51/44C07C59/08C07C67/08C07C67/54C07C69/68
CPCC07C51/48C07C51/09C07C51/44C07C67/08C07C67/54C07C59/08C07C69/68
Inventor 单雨瑶王竞辉刘英俊朱小瑞贾子樊吴计划孙烨沈元伟
Owner WANHUA CHEM (SICHUAN) CO LTD