Lactobacillus paracasei ET-22 for resisting oxidation and regulating blood pressure and application of lactobacillus paracasei ET-22
A kind of Lactobacillus, paracheese technology, applied in Lactobacillus, food ingredients as antioxidants, application and other directions
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Embodiment 1
[0032] Embodiment 1: Determination of growth kinetics characteristics of Lactobacillus paracasei ET-22 fermented milk
[0033] 1. Experimental strains
[0034] Lactobacillus paracasei ET-22 (CGMCC No.15077) used in the technical solution of the present invention;
[0035] Control strain 1: commercial Lactobacillus paracasei strain L.CASEI 431;
[0036] Control strain 2: commercial Lactobacillus paracasei strain LPC-37.
[0037] 2. Activation of the strain and culture of seed solution
[0038] (1) Strain activation: Lactobacillus paracasei was activated with MRS medium, and the strains that had grown to the logarithmic phase were taken and stored in glycerol tubes for future use. Among them, MRS medium (g / L): peptone 10.0g; yeast extract 5.0g; glucose 20.0g; anhydrous sodium acetate 5.0g; dihydroammonium citrate 2.0g; 2.0g; magnesium sulfate heptahydrate 0.2g; manganese sulfate 0.05g; beef extract 10.0g; agar 15g-20g.
[0039] (2) Determination of strain growth dynamics ch...
Embodiment 2
[0079] Example 2. Fermentation of Lactobacillus paracasei ET-22 to prepare a milk beverage with anti-oxidation, blood pressure regulation and sleep regulation potential
[0080] This example provides a milk beverage prepared by fermentation of Lactobacillus paracasei ET-22, which has the potential of anti-oxidation, regulating blood pressure and regulating sleep. The manufacturing steps of this drink are as follows:
[0081] (a) Lactobacillus paracasei ET-22 is inoculated in skim milk and fermented, and fermented milk is obtained after fermentation;
[0082] (b) Blending a sweetener and / or a sour agent to form a blended liquid, mixing the blended liquid with the fermented milk prepared in step (a), and homogenizing to prepare a fermented milk beverage.
[0083] Further, the fermentation temperature in the step (a) is 37°C, and the fermentation time is 96h-120h.
[0084] Further, in step (b), the mixing volume ratio of the fermented milk and the blending solution is 1:2-4.
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