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Method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of bifunctional polyether-ether-ketone

A technology of polyether ether ketone and combined biology, which is applied in the field of medical biomaterials, can solve problems such as functional barriers on the surface of biomolecules, and achieve the effect of antibacterial ability, simple preparation method, and good application prospects

Active Publication Date: 2021-09-07
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PEEK materials have higher chemical resistance than most other polymer biomaterials, which poses a great obstacle to their functionalization on the surface of biomolecules.

Method used

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  • Method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of bifunctional polyether-ether-ketone
  • Method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of bifunctional polyether-ether-ketone
  • Method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of bifunctional polyether-ether-ketone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of bifunctional polyether ether ketone

[0047] 1. Materials: Peptides were prepared using standard Fmoc solid-phase synthesis. PEEK discs (15 mm diameter, 1.0 mm thickness) were purchased from Watson Medical Appliance Co (Changzhou, China), and PEEK rods (1.5 mm diameter, 10 mm length) were purchased from Tianzhu Changyun Medical Technology Co., Ltd. (Beijing, China).

[0048] . Instruments used for characterization: HPLC was performed on a liquid chromatography mass spectrometer (Ekspert TM, Eksigent, USA). ESI-MS was performed on an electrospray ionization mass spectrometer (API 4000+, SCIEX, USA). XPS was performed on an X-ray photoelectron spectrometer (K-Alpha, Thermo Electron Corporation, USA), and AFM images were acquired on an atomic force microscope (Dimension ICON, Bruker Corporation, USA). Static contact angle measurements were performed on a contact angle meter (OCTA21, DATAPHYSICS, Germany).

[0049], Preparation of bifunctional po...

Embodiment 2

[0051] Example 2 Performance Research of Bifunctional Polyetheretherketone

[0052] 1. Cell culture: BM-MSCs came from the Shanghai Cell Bank of the Chinese Academy of Sciences, and were cultured in Dulbecco’s modified Eagle medium (DMEM, HyClone, USA), supplemented with 10% fetal bovine serum (FBS, Gibco, USA), And add 1% penicillin / streptomycin (USA, Gibco). During cell culture, change the medium every three days until reaching 70% to 80% cell coverage on the cell culture dish. 24-well plates were used for seeding, and the cell seeding density of each sample was 2 × 10 4 cells.

[0053] 2. Cytocompatibility: After culturing BM-MSCs on different PEEK samples for 24 hours, cells were stained using a live / dead cell staining kit (AmyJet Scientific, Wuhan, China). Calcein AM (2×10 -6 M) and EthD-1 (10×10 -6 M) Live cells were stained green and dead cells were stained red, respectively, and observed using a confocal laser microscope (TCS SP8, Leica, Germany). LDH and CCK-8...

Embodiment 3

[0064] Embodiment 3 experimental result

[0065] 1. Characterization detection

[0066] XPS was used to detect and analyze the chemical composition of the sample surface. In the XPS spectrum of the PEEK sample, C and O are the dominant elements. After treatment with the mussel-derived click peptide, the N element was introduced, indicating that the N element has been successfully loaded on the surface of the sample. ( figure 1 ).

[0067] AFM results show that the surface of PEEK is relatively flat, and the surface of PEEK-AO becomes very dense and shows uniform undulations, which greatly increases the roughness of the material, thereby increasing the contact area between the material and the cell, and providing a better adhesion. Conditional ( figure 2 ).

[0068] The water contact angle results show that the water contact angle of PEEK is about 80 o , while the water contact angles detected on the modified samples were lower than 40 o . The significant decrease in wa...

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Abstract

The invention discloses a method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of the bifunctional polyether-ether-ketone. The preparation method comprises the following steps: a, synthesizing clickable mussel derived peptide; b, modifying polyether-ether-ketone by the clickable mussel derived peptide; c, preparing the DBCO-AMP and the DBCO-OGP; and d, preparing the bifunctional polyether-ether-ketone. The preparation method is simple and convenient, the preparation cost is relatively low, the prepared bifunctional polyether-ether-ketone (PEEK-AO) is excellent in performance, and an in-vitro cell experiment proves that the PEEK-AO has the effects of promoting adhesion, proliferation and osteogenic differentiation of BMSCs (bone mesenchymal stem cells) and has antibacterial performance on staphylococcus aureus and escherichia coli; in-vivo experiments prove that PEEK-AO promotes implant-bone interface integration by utilizing a rat distal femur implant rod model, has antibacterial ability and has a relatively good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterial application, and in particular relates to a method for preparing bifunctional polyetheretherketone (PEEK) by using mussel-derived peptide combined with bio-orthogonal reaction and its application. Background technique [0002] Polyether ether ketone (PEEK) is a special thermoplastic engineering plastic, which has been widely used in orthopedics and oral and maxillofacial surgery because of its excellent mechanical properties close to cortical bone and extra-bone stability in physiological environment. However, due to the biological inertness of traditional PEEK materials, there are problems of insufficient osseointegration and postoperative bacterial infection, which usually lead to implant loosening and even implant failure, which brings a heavy burden to patients and the whole society. Therefore, orthopedic prostheses made of PEEK need to be further functionalized to improve the oste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G8/28A61L27/18A61L27/22A61L27/54
CPCC08G8/28A61L27/18A61L27/227A61L27/54A61L2300/252A61L2300/404A61L2430/02C08L61/16C08L89/00Y02P20/55
Inventor 耿德春李蒙柏家祥陶华强葛高然肖龙王志荣李文明张巍潘国庆
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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