Method for preparing bifunctional polyether-ether-ketone by combining mussel derived peptide with biological orthogonal reaction and application of bifunctional polyether-ether-ketone
A technology of polyether ether ketone and combined biology, which is applied in the field of medical biomaterials, can solve problems such as functional barriers on the surface of biomolecules, and achieve the effect of antibacterial ability, simple preparation method, and good application prospects
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Embodiment 1
[0046] Example 1 Preparation of bifunctional polyether ether ketone
[0047] 1. Materials: Peptides were prepared using standard Fmoc solid-phase synthesis. PEEK discs (15 mm diameter, 1.0 mm thickness) were purchased from Watson Medical Appliance Co (Changzhou, China), and PEEK rods (1.5 mm diameter, 10 mm length) were purchased from Tianzhu Changyun Medical Technology Co., Ltd. (Beijing, China).
[0048] . Instruments used for characterization: HPLC was performed on a liquid chromatography mass spectrometer (Ekspert TM, Eksigent, USA). ESI-MS was performed on an electrospray ionization mass spectrometer (API 4000+, SCIEX, USA). XPS was performed on an X-ray photoelectron spectrometer (K-Alpha, Thermo Electron Corporation, USA), and AFM images were acquired on an atomic force microscope (Dimension ICON, Bruker Corporation, USA). Static contact angle measurements were performed on a contact angle meter (OCTA21, DATAPHYSICS, Germany).
[0049], Preparation of bifunctional po...
Embodiment 2
[0051] Example 2 Performance Research of Bifunctional Polyetheretherketone
[0052] 1. Cell culture: BM-MSCs came from the Shanghai Cell Bank of the Chinese Academy of Sciences, and were cultured in Dulbecco’s modified Eagle medium (DMEM, HyClone, USA), supplemented with 10% fetal bovine serum (FBS, Gibco, USA), And add 1% penicillin / streptomycin (USA, Gibco). During cell culture, change the medium every three days until reaching 70% to 80% cell coverage on the cell culture dish. 24-well plates were used for seeding, and the cell seeding density of each sample was 2 × 10 4 cells.
[0053] 2. Cytocompatibility: After culturing BM-MSCs on different PEEK samples for 24 hours, cells were stained using a live / dead cell staining kit (AmyJet Scientific, Wuhan, China). Calcein AM (2×10 -6 M) and EthD-1 (10×10 -6 M) Live cells were stained green and dead cells were stained red, respectively, and observed using a confocal laser microscope (TCS SP8, Leica, Germany). LDH and CCK-8...
Embodiment 3
[0064] Embodiment 3 experimental result
[0065] 1. Characterization detection
[0066] XPS was used to detect and analyze the chemical composition of the sample surface. In the XPS spectrum of the PEEK sample, C and O are the dominant elements. After treatment with the mussel-derived click peptide, the N element was introduced, indicating that the N element has been successfully loaded on the surface of the sample. ( figure 1 ).
[0067] AFM results show that the surface of PEEK is relatively flat, and the surface of PEEK-AO becomes very dense and shows uniform undulations, which greatly increases the roughness of the material, thereby increasing the contact area between the material and the cell, and providing a better adhesion. Conditional ( figure 2 ).
[0068] The water contact angle results show that the water contact angle of PEEK is about 80 o , while the water contact angles detected on the modified samples were lower than 40 o . The significant decrease in wa...
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