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Detection reagent and kit used for esophageal cancer diagnosis or auxiliary diagnosis

A detection kit and auxiliary diagnosis technology, applied in the field of biomedicine, can solve the problems of low patient acceptance, inflexible operation, unfavorable prognosis and survival of patients, etc.

Active Publication Date: 2021-09-07
WUHAN AIMISEN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many similar markers that can assist in the diagnosis of tumors, such as P53 antibody, carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag) and cytokeratin 21-1, which can generally obtain certain specificity. The combination of endoscopy and cell biopsy has improved the detection rate of esophageal cancer in high-risk groups, but the acceptance of patients is low and the operation is not flexible enough; imaging examinations mainly There are X-ray barium meal, CT, etc. These examinations have high requirements for instruments and detection techniques, and are expensive, so they are not suitable for large-scale screening
Due to the characteristics of esophageal cancer, most patients will seek medical examination after symptoms such as dysphagia, and most of the esophageal cancers discovered at this time are in a relatively advanced state, which is very unfavorable to the prognosis and survival of patients

Method used

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  • Detection reagent and kit used for esophageal cancer diagnosis or auxiliary diagnosis
  • Detection reagent and kit used for esophageal cancer diagnosis or auxiliary diagnosis
  • Detection reagent and kit used for esophageal cancer diagnosis or auxiliary diagnosis

Examples

Experimental program
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Effect test

Embodiment

[0080] This embodiment provides reagents and detection kits for the diagnosis or auxiliary diagnosis of esophageal cancer, a total of 6 sets, including introduction pairs and probes for amplifying target sequences. The specific sequence is shown in Table 1.

[0081] The target sequence, introduction pair and probe involved in the kit of Table 1 Example 1-6

[0082]

[0083] The esophageal cancer detection kit provided in the above-mentioned Examples 1-6 can be used for detection, diagnosis and auxiliary diagnosis of biological samples of esophageal cancer patients through the following steps:

[0084] 1. Extraction of DNA template:

[0085] When the sample used is an esophageal tissue sample, use the QIAamp DNA FFPE Tissue Kit to extract tissue DNA. For details, refer to the kit instruction manual.

[0086] When the sample used is a blood sample, use Tiangen Biochemical Technology (Beijing) Co., Ltd. Magnetic Bead Method Serum / Plasma Free DNA Extraction Kit (DP709) for pl...

experiment example 1

[0105]48 cases of esophageal squamous cell carcinoma tissues, 48 ​​cases of early stage esophageal squamous cell carcinoma tissues, 64 cases of advanced stage esophageal squamous cell carcinoma tissues and 56 cases of paracancerous tissues diagnosed by clinical pathology in a hospital in Wuhan were collected. All samples were formalin Soaked and paraffin-embedded tissues, the sample collection process was approved by the ethics committee, all patients signed informed consent, and all samples were anonymized. DNA extraction and bisulfite conversion were carried out according to the methods provided in the examples, and the specific primers and probe combinations based on 6 regions in Examples 1-6 were selected for PCR detection. The PCR detection results are shown in Table 4.

[0106] The detection sensitivity and specificity of the detection kit of table 4 embodiment 1-6 in tissue sample

[0107]

[0108]

experiment example 2

[0110] Collect blood samples from 89 cases of esophageal squamous cell carcinoma, 75 cases of early stage esophageal squamous cell carcinoma, 130 cases of advanced stage esophageal squamous cell carcinoma and 52 cases of normal people with clinicopathologically confirmed high-grade neoplasia in a hospital in Zhengzhou. The process was approved by the ethics committee, all patients signed informed consent, and all samples were anonymized. Carry out plasma DNA extraction and bisulfite conversion according to the method provided in the embodiment, select the combination of specific primers and probes based on 6 regions in the human genome for PCR detection in Examples 1-6, the PCR detection results are shown in the table 5.

[0111] The detection sensitivity and specificity of the kit of table 5 embodiment 1-6 in plasma sample

[0112]

[0113] As can be seen from Table 4 and Table 5, when the test kit of embodiment 1-embodiment 6 is used as the detection and diagnosis of pat...

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Abstract

The invention discloses a detection reagent and kit used for esophageal cancer diagnosis or auxiliary diagnosis, is used for carrying out DNA methylation level detection on the full length and partial region of SEQ ID NO.22 or a complementary sequence thereof, can be used for diagnosis and auxiliary diagnosis of esophageal cancer patients, and is especially suitable for detection diagnosis / auxiliary diagnosis of esophageal squamous cell carcinoma. The detection reagent and kit can be used for esophageal cancer patients in different stages, has good sensitivity and specificity, can adopt tissue samples and plasma samples as monitoring samples, and has great significance in early diagnosis of the esophageal cancer and improvement of patient prognosis.

Description

technical field [0001] The present application relates to the technical field of biomedicine, in particular to a detection reagent and kit for diagnosis or auxiliary diagnosis of esophageal cancer. Background technique [0002] Esophageal cancer is a malignant tumor that occurs in the esophageal mucosal epithelium and esophageal glandular epithelium. It is a very common malignant tumor. In recent years, the incidence of esophageal cancer is still gradually increasing. At present, the prevalence of esophageal cancer in my country ranks first in the world and far exceeds that of other countries and regions. In terms of all tumors, esophageal cancer is the fourth most malignant tumor in my country after lung cancer, liver cancer and gastric cancer. Early diagnosis and early treatment intervention of esophageal cancer are of great significance to reduce the mortality of esophageal cancer and prolong the survival period of patients. [0003] There are two main histological clas...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154C12Q2600/112C12Q2600/118
Inventor 张良禄周俊董兰兰熊杨辉李婷婷
Owner WUHAN AIMISEN LIFE TECH CO LTD
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