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FeOOH/PVDF (Polyvinylidene Fluoride) fiber scaffold as well as preparation method and application thereof

A fiber scaffold and fiber membrane technology, applied in biochemical equipment and methods, fiber processing, filament/thread forming, etc., can solve the problem of weak influence of stem cell differentiation

Active Publication Date: 2021-09-10
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effect of electrical stimulation alone on stem cell differentiation is relatively weak, and how to use piezoelectric materials to prepare materials more suitable for clinical applications has become a thorny issue

Method used

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  • FeOOH/PVDF (Polyvinylidene Fluoride) fiber scaffold as well as preparation method and application thereof
  • FeOOH/PVDF (Polyvinylidene Fluoride) fiber scaffold as well as preparation method and application thereof
  • FeOOH/PVDF (Polyvinylidene Fluoride) fiber scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of FeOOH / PVDF fiber scaffold

[0038] (1) Dissolve PVDF powder (Solvay 6010) in a mixed solvent of acetone: N,N-dimethylformamide = 3:7 (v / v), and configure a PVDF solution with a mass fraction of 12%; use a syringe to draw Insert about 5ml of PVDF solution into a 22G needle, and set the spinning parameters as follows: distance 15cm, voltage 18kV. After spinning, remove the membrane and dry it in an oven at 60°C for 30min to obtain a PVDF fiber membrane. The SEM Figure such as figure 1 As shown in a: the PVDF fiber surface is smooth and uniform in thickness, with a diameter of about 600nm;

[0039] (2) Weigh ferric chloride hexahydrate and sodium nitrate and dissolve them in 50 mL of distilled water so that the concentration of ferric chloride is 0.1 M and the concentration of sodium nitrate is 1 M. Add aqueous hydrochloric acid to adjust the pH to 1.25; add step (1) to prepare The good PVDF fiber membrane (size 2 cm×4 cm) was transferred to the ...

Embodiment 2

[0042] Example 2 Preparation of FeOOH / PVDF fiber scaffold

[0043] (1) The preparation of the PVDF membrane is the same as in Example 1 (1);

[0044] (2) Weigh ferric chloride hexahydrate and sodium nitrate and dissolve them in 50 mL of distilled water so that the concentration of ferric chloride is 0.12M and the concentration of sodium nitrate is 1M. Add aqueous hydrochloric acid to adjust the pH to 1.25; add step (1) to prepare The PVDF fiber membrane (about 2 cm × 4 cm) was transferred to the hydrothermal reaction kettle together, and then reacted at 95 ° C for 12 h, washed three times alternately with water and absolute ethanol, and dried to obtain the FeOOH / PVDF fiber scaffold. The TEM image of iron oxyhydroxide is shown in figure 1 As shown in a and b: FeOOH is rod-shaped, the crystal form is β-type, the size is uniform, the length is about 900nm, the width is about 100nm, and it has good crystallinity. SEM image of FeOOH / PVDF fiber scaffold Figure 5 As shown in c an...

Embodiment 3

[0045] Example 3 Preparation of FeOOH / PVDF fiber scaffold

[0046] (1) Dissolve PVDF powder (Solvay 6010) in a mixed solvent of acetone: N,N-dimethylformamide = 4:6 (v / v), and configure a PVDF solution with a mass fraction of 15%; use a syringe to draw Insert about 5ml of PVDF solution into a 22G needle, set the spinning parameters as follows: distance 15cm, voltage 20kV, after spinning, remove the membrane and dry it in a 60°C oven for 30min to obtain a PVDF fiber membrane, its SEM Figure such as Figure 8 shown. It can be seen from the figure that the PVDF fiber surface is smooth and uniform in thickness, with a diameter of about 600nm;

[0047] (2) The synthesis of FeOOH is the same as in Example 1 (2). PVDF membrane and FeOOH / PVDF membrane are carried out PFM test in step 1, the result is as follows Figure 9 It is shown that FeOOH / PVDF has good piezoelectricity, and at the same time, compared with PVDF, the piezoelectricity does not weaken significantly after loading ...

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Abstract

The invention provides a preparation method of a FeOOH / PVDF (Polyvinylidene Fluoride) fiber scaffold. The preparation method of the FeOOH / PVDF fiber scaffold comprises the following steps of dissolving PVDF powder in a mixed solvent of acetone and N, N-dimethylformamide to prepare a PVDF solution, then carrying out electrostatic spinning to obtain a fiber membrane, and then drying to obtain a PVDF fiber membrane; and transferring a mixed solution of ferric chloride hexahydrate and sodium nitrate and the PVDF fiber membrane into a reaction kettle for hydrothermal reaction, and washing and drying a product to obtain the FeOOH / PVDF fiber scaffold. The FeOOH in the FeOOH / PVDF fiber scaffold obtained by the preparation method is rod-shaped, the length is about 800 to 900 nm, the width is about 100 nm, and the crystal form is beta form; and the FeOOH is loaded on the surface of the PVDF fiber. The FeOOH / PVDF fiber scaffold can be used as a medical material for inducing mesenchymal stem cells to differentiate into nerve cells. According to the FeOOH / PVDF fiber scaffold as well as the preparation method and the application thereof provided by the invention, the FeOOH / PVDF fiber membrane is designed and prepared by utilizing an electrostatic spinning technology and a hydrothermal reaction process, the preparation method is simple, and reaction conditions are easy to realize; and the obtained scaffold is uniform in surface appearance, has good piezoelectricity, and can induce rBMSCs neural differentiation in a wireless stimulation mode.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method and application of FeOOH / PVDF fiber scaffold for inducing directional differentiation of mesenchymal stem cells into functional nerve cells by electrical stimulation without wires under the action of ultrasound. Background technique [0002] Population aging, cardiovascular and cerebrovascular diseases, and neurodegenerative diseases caused by traffic accidents have become increasingly serious threats to human life and health. Although medical scientists and clinicians have made great efforts and made great progress, nerve repair and regeneration still face great challenges in clinical application. Among them, the main problem is the lack of enough functional neurons. Currently, a promising approach is to induce neural stem cells to differentiate into functional neurons to restore the function of the nervous system. However, due to issues o...

Claims

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Application Information

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IPC IPC(8): D04H1/728D01D5/00C12N5/0793C12N13/00
CPCD04H1/728D01D5/003D01D5/0084C12N5/0619C12N13/00D10B2509/00C12N2506/1353C12N2521/10C12N2533/30
Inventor 王金刚韩树伟张瑞彤孙春辉刘宏
Owner UNIV OF JINAN
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