Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermentation and cultivation method of schizophyllum producing schizophyllum polysaccharide

A technology for fermenting and culturing Schizophyllum, applied in the field of microbial fermentation, can solve problems such as the inability to meet the needs of polysaccharide production, and achieve the effects of increasing yield, increasing content and increasing yield

Active Publication Date: 2022-04-01
WUZHOUFENG AGRI SCI & TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both jasmonic acid and methyl jasmonate increase polysaccharide production by increasing the activity of glycosyltransferases (Jaulneau, Valérie, et al."Ulvan, a sulfated polysaccharide from green algae, activates plant immunity through the jasmonic acid signaling pathway. "Journal of Biomedicine and Biotechnology 2010(2010).), but still can not meet the demand of polysaccharide production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation and cultivation method of schizophyllum producing schizophyllum polysaccharide
  • Fermentation and cultivation method of schizophyllum producing schizophyllum polysaccharide
  • Fermentation and cultivation method of schizophyllum producing schizophyllum polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Preparation of soybean meal protein.

[0028] The following four schemes A, B, C, and D are used to prepare soybean meal protein respectively, and the parts mentioned refer to parts by mass.

[0029] A: Soak 80 parts of soybean meal with 300 parts of water for 24 hours; adjust the pH value to 6-7, add papain according to 2% of the weight of soybean meal, stir evenly, and carry out enzymatic hydrolysis at 55° C. for 0.5 hour. The enzymatic hydrolyzate was deactivated at 100° C. for 10 minutes, and soybean meal protein A was obtained after the treatment.

[0030] B: Soak 80 parts of soybean meal in 300 parts of water for 24 hours; adjust the pH value to 6-7, add papain according to 2% of the weight of soybean meal, stir evenly, and carry out enzymatic hydrolysis at 55° C. for 1 hour. The enzymolyzate was deactivated at 100° C. for 10 minutes, and soybean meal protein B was obtained after the treatment.

[0031] C: Soak 80 parts of soybean meal in 300 parts...

Embodiment 2

[0034] Example 2: Initial culture of Schizophyllum.

[0035] Schizophyllum commune SCSIO z046 was inoculated with PDA comprehensive medium (potato 200g, glucose 20g, agar 15-20g, water 1000mL, pH natural), and cultured at 30°C in the dark for 5-10 days after inoculation.

Embodiment 3

[0036] Example 3: Optimization of SPG basal medium.

[0037] The production of SPG was optimized using the experimental method of Design Expert version 7.0.1.0 (Statcon, USA). The independent variables selected for optimization were as follows: starch, soybean meal protein prepared by Protocol C of Example 1, yeast extract, sodium carboxymethylcellulose (CMC) and oleic acid. Table 1 presents the factors evaluated at five different levels. The total experiment was 50 runs, including 32(2 5 ) factor points, 10 axial points, and 8 replicates of the center point. The dependent response is the SPG yield (y 1 ) and biomass production (y 2 ).

[0038] Table 1: Experimental independent variables and their levels in the central composite design (CCD)

[0039]

[0040] Effective factors such as carbon and nitrogen sources and appropriate concentration ranges were investigated in this study through a one-factor-at-a-time approach, as considered in previous studies. Based on the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of using microorganisms to produce high value-added products, and in particular relates to a method for fermenting and culturing Schizophyllum polysaccharides from Schizophyllum, the method comprising the following steps: using basic culture medium to carry out deep layer liquid culture of Schizophyllum, and then adding ring Pentanone fatty acids continued to be cultured. The fermentation medium and the cyclopentanone fatty acid additive provided by the invention can shorten the fermentation cycle of the schizophyllum hyphae and greatly increase the content of high value-added products produced by the unit mycelium.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for promoting high-level secretion of schizophyllum polysaccharide by schizophyllum. Background technique [0002] Polysaccharides are abundant natural biopolymers and represent the largest polymers ever produced globally. Schizophyllan polysaccharide (SPG), as a biopolymer, is a nonionic and water-soluble extracellular polysaccharide consisting of a backbone of 1,3-β-D-glucopyranose units linked to a single 1,6- β-D-glucopyranose linking. This biopolymer is produced by the wood-decaying and filamentous fungus Schizophyllum strains (Kumari, Maushmi, Shrikant A. Survase, and Rekha S. Singhal. "Production of schizophyllan using Schizophyllum commune NRCM." Bioresource technology 99.5 (2008): 1036-1043. Alizadeh, Vahid, Seyed AbbasShojaosadati, and Seyed Morteza Zamir."Enhancement of schizophyllan production in Schizophyllum commune using microparticles i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/14C12P19/04C12R1/645
CPCC12N1/38C12N1/14C12P19/04
Inventor 迟艳李峰刘昌春张宇
Owner WUZHOUFENG AGRI SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com