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Preparation and application of Pluronic-F127 hydrogel loaded nano sponge detoxification system

A technology of pluronic-f127 and nano-sponge, which is applied in blood/immune system cells, cell dissociation methods, biochemical equipment and methods, etc., can solve problems that have not been reported yet, and achieve the effect of simple preparation process

Inactive Publication Date: 2021-10-08
中国人民解放军海军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the preparation and application of Pluronic F-127 hydrogel-loaded nano-sponge detoxification system

Method used

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  • Preparation and application of Pluronic-F127 hydrogel loaded nano sponge detoxification system
  • Preparation and application of Pluronic-F127 hydrogel loaded nano sponge detoxification system
  • Preparation and application of Pluronic-F127 hydrogel loaded nano sponge detoxification system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the preparation of NS

[0043] ①Preparation of PLGA nanoparticles: 0.67dl / g 50:50 PLGA polymer containing carboxy-terminal was prepared into PLGA nanoparticles of about 60nm by nanoprecipitation method. First, PLGA was dissolved in acetone to prepare a PLGA acetone solution (5 mg / ml); then, 1 ml of PLGA acetone solution was added to 3 ml of deionized water, stirred at room temperature for 3 hours, and the residual acetone was removed in vacuum to obtain PLGA nanoparticles. In addition, for the preparation of DiD fluorescent dye-labeled NSs for subsequent tracking imaging, except that 0.1wt% DiD fluorescent dye was added to PLGA acetone solution, the rest of the steps refer to the preparation of blank PLGA nanoparticles. The hydrated particle size and zeta potential of PLGA nanoparticles were measured by DLS. Observed under the transmission electron microscope, it can be seen that the typical nano-scale particle structure ( figure 1 ).

[0044]② Preparat...

Embodiment 2

[0046] Example 2: Preparation and characterization of Pluronic-F127 hydrogel-loaded NS detoxification system

[0047] ①Pluronic-F127 hydrogel: prepare Pluronic-F127 hydrogel solution according to the concentration of 10%, 15%, 20%, 25% and 30%, fully dissolve at 4°C overnight to remove air bubbles, observe the gelation in a 37°C water bath , it can be seen that the Pluronic-F127 hydrogels with a concentration of 25% and 30% can phase into gel ( image 3 ).

[0048] ②Mix the final concentration of 1mg / ml NS and 300mg / mL Pluronic-F127, fully dissolve at 4°C overnight to remove air bubbles, and observe the gelation in a 37°C water bath. It can be seen that the loading of NS does not affect the gelation of 30% Pluronic-F127 ( image 3 ), and a typical porous network structure under the scanning electron microscope ( Figure 4 ). Rheological tests show that the viscosity of the hydrogel-NS system is extremely low below 15°C, and can quickly phase-change to gel at 25°C, and the ...

Embodiment 3

[0051] Example 3: Pluronic-F127 hydrogel-loaded NS detoxification system inhibits hemolytic effect in vitro

[0052] First, the hemolytic activity of VvhA and Hlα was determined using a 2% suspension of mouse erythrocytes. Then, mix different concentrations of NS (0.016-2 mg / mL, final volume 0.5 mL) with VvhA (15 μg / mL) or Hlα (1 μg / mL), and then add an equal amount of 2% RBC suspension, at 37 ° C Incubate for 30min. After the incubation, centrifuge at 1000rpm for 5min, take the supernatant, and detect the absorbance of the released hemoglobin at 540nm. A similar hemolysis inhibition experiment was used to test the toxin neutralization ability of the Pluronic-F127 hydrogel-loaded nanosponge detoxification system (containing 1 mg / mL NS), and blank gel, NS and PBS were used as controls.

[0053] The results show( Figure 8 ), 15 μg / mL of VvhA or 1 μg / mL of Hlα can cause complete hemolysis; 1 mg / mL of NS can completely inhibit the hemolytic effect of the two toxins at the same...

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Abstract

The invention relates to the technical field of nano medicines, in particular to a Pluronic-F127 hydrogel loaded nano sponge (NS) detoxification system and a preparation method thereof. The Pluronic-F127 hydrogel loaded nano sponge detoxification system is obtained by wrapping PLGA nanoparticles with erythrocyte membrane vesicles to prepare NS, and then loading the NS with Pluronic-F127 hydrogel. The invention also provides an application of the hydrogel loaded nano sponge detoxification system in treatment of drug-resistant bacterium infection. According to the Pluronic-F127 hydrogel loaded nano sponge detoxification system and the preparation method thereof, the adopted Pluronic-F127 hydrogel is a biocompatible material approved by FDA and applicable to a human body, can load the NS to form the detoxification system with a local toxin adsorption effect, and can be applied to the fields of biological medicines, medical auxiliary materials and the like.

Description

technical field [0001] The invention relates to the technical field of nanomedicine, in particular to the preparation and application of a Pluronic-F127 hydrogel-loaded nano-sponge detoxification system. Background technique [0002] Drug-resistant bacterial infections have become a global public health problem due to their high morbidity and mortality. According to the statistics of the World Health Organization, about 700,000 people die from drug-resistant bacterial infections every year in the world (Genovese C, La Fauci V, Damato S, et al. Molecular epidemiology of antimicrobial resistant microorganisms in the 21th century: a review of the literature[J ].Acta Biomed,2020,91(2):256-73; Christaki E,Marcou M,Tofarides A.Antimicrobial resistance in bacteria: mechanisms,evolution,and persistence[J].J Mol Evol,2020,88(1): 26-40.), there is an urgent need to develop new therapeutic strategies. Targeted anti-drug has become a new alternative treatment method. Among them, the ...

Claims

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Application Information

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IPC IPC(8): A61L26/00C12N5/078C12N13/00
CPCA61L26/0019A61L26/0057A61L26/008A61L26/0066A61L26/0085C12N5/0641C12N13/00A61L2300/30A61L2300/404A61L2400/12C12N2509/10C08L67/04C08L71/02
Inventor 张黎明王蓓蕾邹帅军王倩倩柳国艳王博张福海李捷王钒
Owner 中国人民解放军海军特色医学中心
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