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Salinivibrio for removing calcium ions in high-salinity wastewater and application of salinivibrio

A high-salt wastewater and halovibrio technology, applied in the field of environmental microorganisms, can solve many problems, such as improving building materials, broad application prospects, and increasing strength

Active Publication Date: 2021-10-15
SHANDONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on the immobilization and biomineralization of microorganisms in high-salt environments to precipitate calcium ions.

Method used

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  • Salinivibrio for removing calcium ions in high-salinity wastewater and application of salinivibrio
  • Salinivibrio for removing calcium ions in high-salinity wastewater and application of salinivibrio
  • Salinivibrio for removing calcium ions in high-salinity wastewater and application of salinivibrio

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Experimental program
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Effect test

Embodiment 1

[0037] Culture, identification and preservation of bacteria

[0038] 1. Culture of bacteria

[0039] In this study, after separating and purifying halophilic bacteria from the seawater collected by Yinjiashan Salt Factory, the halovibrio bacterial strain of the present invention is obtained, and the liquid medium for cultivating HMY2 bacteria contains the following components: tryptone (10g L -1 ), beef extract (5g·L -1 ), NaCl (100g·L -1 ), KCl (2g·L -1 ), 1L of distilled water, and finally adjust the pH range of the liquid medium to 7.2-7.4. Add agar (2g L -1 ) powder to prepare solid medium.

[0040]Prepare the above-mentioned liquid medium after autoclave sterilization and cooling, take 10mL water sample (collected from Yinjiashan Salt Field, Qingdao, China) and add it to 150mL liquid medium, and place it in a shaker for enrichment culture with a rotation speed of 120rpm and a temperature of 32°C. After 48 hours, the liquid medium became turbid, confirming that the e...

Embodiment 2

[0047] Preparation of halovibrio HMY2 bacterial agent

[0048] Halovibrio HMY2 was activated on solid medium, and then a single colony was picked and inoculated into a 250mL Erlenmeyer flask containing 150mL of liquid medium, and cultured on a constant temperature shaker at 32°C and 120rpm for 120h to obtain OD 600 =1 Halovibrio mesolongii (Halovibrio mesolongii) HMY2 bacterial agent, referred to as HMY2 bacterial agent.

Embodiment 3

[0050] Micromorphological Analysis of Halovibrio HMY2

[0051] Take a drop of HMY2 bacteria agent and drop it into the copper grid containing carbon film. After the water in the copper grid volatilizes naturally, use a high-resolution transmission electron microscope HRTEM to observe the morphology and element composition of Halovibrio HMY2 in the liquid medium .

[0052] figure 2 The microscopic morphology of Halovibrio HMY2 was observed by HRTEM, and it was found that the length of the bacteria was 2-4 μm, the width was 0.3-0.5 μm, and there were extracellular polymers (EPS) around the bacteria. The distribution of Ca, C, O, Mg, N, S and P elements in and around a single Halovibrio HMY2 was analyzed, among which the Ca element was evenly distributed, and the distribution of C, O, N, and P elements in the bacteria was significantly more The distribution of Mg and S elements in the cell is less, and the EDS analysis of the cell interior of Halovibrio HMY2 shows that the con...

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Abstract

The invention provides a salinivibrio for removing calcium ions in high-salinity wastewater and application of the salinivibrio. The bacterial strain is named as Halovibrio mesolongii HMY2, and is preserved in the China General Microbiological Culture Collection Center on April 13, 2021, and the preservation number is CGMCC No.22182. The halovibrio disclosed by the invention has a wide application prospect in the field of high-salt hard water treatment, calcium ions in the high-salt hard water can be settled so as to achieve the purpose of reducing the hardness of the high-salt hard water, and in addition, the formed calcium carbonate precipitate has an extremely important resource utilization value and can be used for improving the strength of concrete, improving building materials and the like.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a halovibrio strain for removing calcium ions in high-salt wastewater and an application thereof. Background technique [0002] my country is rich in oil, natural gas and well salt resources, and a large amount of high-salt hard water will be produced during the mining process. If it is directly reused, scale will appear on the equipment and pipelines, or even be blocked by scale, which will not only affect the service life of the pipeline, but also cause serious consequences. If it is discharged directly, it will not only cause waste of water resources, but also pollute the water environment. Therefore, how to efficiently treat high-salt hard water is not only beneficial to the recycling of water resources, energy saving and emission reduction, but also crucial to dealing with current environmental and water resource issues. [0003] In industry, chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/084C12N11/04C02F3/34C12P3/00C04B22/10C01F11/18C12R1/01C02F101/10
CPCC12N1/20C12N11/14C12N11/084C12N11/04C02F3/34C12P3/00C04B22/10C01F11/18C02F2101/10Y02W10/10
Inventor 闫华晓黄美玉赵辉韩作振徐毓东王纪晗魏莉荣李丹
Owner SHANDONG UNIV OF SCI & TECH