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Application of a traditional Chinese medicine composition in the preparation of anti-cervical precancerous lesions

A composition and technology for cervical cancer, applied in the direction of drug combination, drug delivery, antiviral agents, etc., can solve the problems of high recurrence rate, difficulty in evaluation, low efficiency and easy recurrence, etc., and achieve the goal of inhibiting cervical precancerous lesions and exempting surgery Bitterness, the effect of ensuring reproductive function

Active Publication Date: 2022-06-28
苏州龙达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing types of anti-HPV products mainly include: α-interferon immunomodulatory type, which does not directly affect the virus and enhances the immune response, with unsatisfactory curative effect and high recurrence rate; corrosive chemical topical drugs, which are ineffective and easy to relapse, and are effective against high-risk HPV Viruses are ineffective; biological protein dressing type, biological protein charge non-specific combination, mainly plays a physical isolation role, has limited direct effect on viruses, and the curative effect varies too much from individual to individual, making it difficult to evaluate; most products are ineffective against persistent infection, and most of them have no clinical symptoms HPV infection is transient, and the infected person will heal itself without using the product, so most of the existing products have fished in troubled waters and are ineffective for persistent infection

Method used

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  • Application of a traditional Chinese medicine composition in the preparation of anti-cervical precancerous lesions
  • Application of a traditional Chinese medicine composition in the preparation of anti-cervical precancerous lesions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of Chinese medicine composition of the present invention

[0029] S1. Take 5 parts of Solanum nigrum, 12 parts of Sophora flavescens, 8.5 parts of Andrographis paniculata, 2 parts of Rhubarb and 8.5 parts of Indigo Naturalis, washed, dried at 40°C for 15h, and then pulverized at 10000r / min for 60s to obtain coarse powder.

[0030] S2. The coarse powder obtained in step S1 is crushed by a vibrating grinding type wall-breaking ultrafine pulverizer under the condition of 960 r / min for 40 minutes, the temperature is -15 ° C, and the particle size is below 25 microns to obtain ultra-fine wall-breaking powder. ; Ultrafine broken powder is sterilized by cobalt 60 rays.

[0031] S3. First, add 1 part of carbomer, 1 part to 2 parts of small molecular weight sodium hyaluronate, 0.1 part of sodium ethylparaben, add distilled water to 85 parts, and fully swell to obtain a gel matrix. Add gel matrix to 15 parts of ultra-fine wall-breaking powder in ste...

Embodiment 2

[0033] Example 2: Pharmacological and pharmacodynamic experiments

[0034] The antiviral activity of the gel paste prepared in Example 1 was detected, and the activity against human papilloma virus HPV16 was mainly detected. The detection basis is "Disinfection Technical Specifications" (2002 edition), taking Vero-E6 cells as target cells, using the gel paste prepared in Example 1 as a test group, using step S3 in Example 1, without adding 15 parts of ultrafine The gel matrix prepared by the broken wall powder was used as the control group. Three parallel experiments were set up in the experimental group and the control group. Human papilloma virus HPV16 was added to the normal growth medium of the target cell Vero-E6 cells for co-incubation, and then the coagulation was added. The gel paste or gel matrix was used to observe the inhibitory effect of the gel paste or gel matrix on human papillomavirus HPV16. The test results are shown in the following table. The results show tha...

Embodiment 3

[0037] Example 3: Toxicology Experiment

[0038] This example illustrates the safety of the gel paste based on in vitro cytotoxicity:

[0039] In this example, Vero-E6 cells were used as target cells and incubated with media containing different concentrations of gel paste. The results showed that different drug doses had different cytotoxicity to Vero-E6 cells, and cell viability was significantly related to the concentration of gel paste. Inversely proportional, the results are shown in the following table:

[0040] Table 2

[0041]

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PUM

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Abstract

The invention discloses a traditional Chinese medicine composition for resisting HPV infection and a preparation method thereof. The traditional Chinese medicine composition of the invention is prepared from the following raw materials: black nightshade, flavescens, andrographis paniculata, rhubarb and indigo naturalis. The active ingredients in the traditional Chinese medicine composition of the present invention fully contact the vagina and cervical mucous membrane with high permeability, directly reach the cells of the basal layer, can kill the virus more thoroughly and deeper, have a significant curative effect on HPV infection, and the negative conversion rate reaches more than 88% , effectively inhibit the precancerous lesions of the cervix, and make the prognosis of lesions below CINⅡ grade. And the effective rate of treating chronic inflammation such as cervical erosion and vaginitis is over 98%.

Description

technical field [0001] The invention relates to the application of a traditional Chinese medicine composition in the preparation of an anti-cervical precancerous lesion drug, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Cervical cancer is one of the three major malignant tumors of the female reproductive system, and it is the fourth cancer with the highest morbidity and mortality in women worldwide. The number of new cases in China is 130,000 per year. The incidence of cervical cancer is closely related to persistent infection of high-risk human papillomavirus (HR-HPV). HPV can be detected in all invasive cervical cancer and 95% of cervical intraepithelial neoplasia and carcinoma in situ. The high-risk HPV types are mainly HPV16 and HPV18. The main oncogenic proteins E6 and E7 expressed by HPV play a key role in the development of cervical cancer from HPV infection. 70-80% of women have been infected with HPV in their lif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K36/81A61K47/32A61K47/36A61P15/00A61P29/00A61P31/20A61P35/00
CPCA61K36/81A61K36/489A61K36/19A61K36/708A61K36/704A61K36/315A61K36/195A61K9/06A61K47/32A61K47/36A61K9/0034A61P31/20A61P35/00A61P15/00A61P29/00A61K2236/15A61K2300/00
Inventor 李丽娅尹斯佳李丽琦
Owner 苏州龙达生物科技有限公司
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