Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pig brain proliferation promoting peptide-brain phospholipid co-production method

A pig brain and phospholipid technology, which is applied to the preparation methods of peptides, chemical instruments and methods, edible phospholipid compositions, etc., can solve the problems of complicated preparation process, influence on product quality, metal ion pollution, etc., and achieves simple preparation process and low cost. , Environmentally friendly effect

Pending Publication Date: 2021-10-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inorganic salt composite precipitation method utilizes the selectivity of inorganic salts to phospholipid molecules. Although it can greatly increase the content of lecithin, it will introduce metal ions during operation, which will affect product quality and cause metal ion pollution.
[0006] Therefore, the existing preparation of proliferative peptides and phospholipids in the brain using pig brain as raw material has the problems of cumbersome preparation process, destruction of amino acids, difficult control of the reaction process, impact on the environment and impact on product quality.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pig brain proliferation promoting peptide-brain phospholipid co-production method
  • Pig brain proliferation promoting peptide-brain phospholipid co-production method
  • Pig brain proliferation promoting peptide-brain phospholipid co-production method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0065] Wherein the preparation steps of porcine brain proliferation-promoting peptide are,

[0066](d) ultrafiltration is carried out by successively passing the filtrate I in step (c) through a cellulose membrane with a molecular weight of 5000Da~6000Da and a cellulose membrane with a molecular weight of 950Da~1500Da; the inlet pressure in the ultrafiltration process is 2.81Bar~3.05 Bar, the reflux pressure in the ultrafiltration process is 0.53Bar~0.67Bar.

[0067] (e) vacuum drying and dehydrating the ultrafiltered substance in step (d) to obtain extract I, which is porcine brain proliferation-promoting peptide.

[0068] Wherein the more specific preparation steps of porcine brain proliferation-promoting peptide are,

[0069] (d1) ultrafiltration of the filtrate I in step (c) through a cellulose membrane with a molecular weight of 5000Da~6000Da; continuous ultrafiltration until the volume of the filtrate I becomes 1 / 3.5~1 / 3 of the original, to obtain a retentate I and the...

Embodiment 1

[0122] The porcine brain proliferation-promoting peptide-brain phospholipid co-production method includes the following steps,

[0123] (a) select 100g porcine brain and 900ml physiological saline, mix the selected porcine brain and physiological saline in proportion and homogenize for 15min;

[0124] (b) 8U / mg trypsin was added to the homogenized mixture to carry out stirring enzymolysis, the stirring time was 25min, the pH value of the solution in the enzymolysis process was 6-8, the enzymolysis temperature was 45°C, and the enzymolysis time was 5h;

[0125] (c) Filtration I and filter residue I are obtained after the material after enzymolysis is filtered.

[0126] includes steps,

[0127] (f) filter residue I in step (c) is carried out freeze dehydration, get the cold acetone that temperature is -15 ℃~-2 ℃, the filter residue I after cold acetone and freeze dehydration is mixed, the volume consumption of cold acetone is freezing 2.5 times of filter residue 1 volume after...

Embodiment 2

[0136] The volume ratio of ethanol to ammonia water in the alkali ethanol solution is 100:0.5, and other steps and data are the same as in Example 1.

[0137] The contents of phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) in the phospholipid extract of brain detected by HPLC are shown in Table 5;

[0138] Table 5: When the volume ratio of ethanol to ammonia water is 100:0.5, the content of components in the phospholipid extract in the brain

[0139]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of food and medicine ingredients, and particularly discloses a pig brain proliferation promoting peptide-brain phospholipid co-production method, which comprises the following steps: (a) selecting a pig brain and normal saline, mixing the selected pig brain and normal saline in proportion, and homogenizing; (b) adding trypsin into the homogenized mixture, and stirring for enzymolysis; and (c) filtering the substances subjected to enzymolysis to obtain filtrate I and filter residues I. The method has the characteristics that the preparation process is simple, the reaction process is mild, and the product quality is not influenced.

Description

technical field [0001] The invention relates to the technical field of food and medicine ingredients, in particular to a method for co-producing porcine brain proliferation-promoting peptide-brain phospholipids. Background technique [0002] Pig brain is not only fresh and delicious, but also rich in protein, phospholipids and various minerals, and can also increase the body's immunity. It is a traditional brain food. [0003] Pig brain hydrolyzate can be used to treat nervous system diseases and prevent age-dependent dementia. Clinically, Ziganshina L E, Abakumova T, and Vernay L found that pig brain polypeptides can improve the symptoms of patients with acute ischemic stroke and delay the progression of the disease. Studies have shown that pig brain polypeptide has a neuroprotective effect on NSC-34 cells, but too high a concentration of pig brain polypeptide will inhibit cell proliferation. Sharma H S, Zimmermann-Meinzingen S, Johanson C E found that pig brain hydrolyza...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07F9/10
CPCC12P21/06C07K1/34C07F9/103
Inventor 谌迪肖朝耿卢文静叶沁张岑张治国
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com