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Biological algaecide as well as preparation method and application thereof

An algaecide and biological technology, applied in the field of water treatment, can solve the problems of difficult application of blue-green algae control, water biological hazards, secondary pollution, etc., and achieve the effects of improving algae removal effect, strong ability to tolerate environmental changes, and low cost

Pending Publication Date: 2021-10-29
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods mainly include mechanical dredging, filter algae removal, clay algae removal and air flotation algae removal, etc., which are expensive and costly, and it is difficult to apply large-scale cyanobacteria control, which is limited in practical application; chemical methods mainly use chemical Chemicals such as copper sulfate, hydrogen peroxide, surfactants, sodium hypochlorite, etc., this method is prone to secondary pollution and has potential hazards to aquatic organisms

Method used

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  • Biological algaecide as well as preparation method and application thereof
  • Biological algaecide as well as preparation method and application thereof
  • Biological algaecide as well as preparation method and application thereof

Examples

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preparation example Construction

[0034] In a typical implementation of the present invention, a method for preparing the above-mentioned biological algicide comprises the following steps:

[0035] (1) Fermenting and culturing the pre-prepared compound bacterial agent to obtain a compound bacterial liquid;

[0036] (2) Mixing the composite bacterial solution in step (1) with the chitosan solution in proportion, and modifying the composite bacterial agent with chitosan to obtain a biological algicide.

[0037] Further, in described step (1), the fermentation culture method of composite bacterial agent comprises the following steps:

[0038] A1. Inoculate the compound bacterial agent into the shake flask containing the culture medium, and shake and culture in an incubator at a temperature of 20-40°C for 24-48 hours to obtain a first-grade seed liquid, and control the rotation speed at 100-200rpm;

[0039] A2. Inoculate the primary seed liquid obtained in step A1 into a fermenter with an inoculum volume fraction o...

Embodiment 1

[0049] In the present embodiment, the fermentation culture method of composite bacterial agent comprises the following steps:

[0050] A1. Inoculate the compound microbial agent comprising actinomycetes, bacillus, alteromonas, and myxobacteria into a shake flask containing a culture medium, the inoculum amount is 10%, and culture in a 30° C. incubator with shaking for 24 hours to obtain a Grade seed liquid, and control rotating speed at 150rpm; Described composite bacterial agent comprises the following components by weight: 4 parts of Actinomycetes, 4 parts of Bacillus, 2 parts of Pseudomonas alternata, 0.8 part of Myxobacteria.

[0051] A2. Inoculate the seed liquid obtained in step A1 into a fermenter according to the volume fraction of 10% inoculum. The culture conditions of the fermenter are 30° C. of temperature, 150 rpm of rotation speed, pH 6.8, and 30% of dissolved oxygen for semi-continuous culture. fermentation.

[0052] Further, the semi-continuous fermentation st...

Embodiment 2

[0056] In the present embodiment, adopt the method for chitosan modified composite bacterial agent to comprise the steps:

[0057] Preparation of B1, chitosan solution

[0058] Weigh 100 mg of chitosan, add 20 mL of distilled water, drop a small amount of glacial acetic acid to dissolve it, and finally make up the volume to 100 mL to obtain a 1 mg / mL chitosan solution.

[0059] B2, chitosan modified compound bacterial agent

[0060] Stir and mix the composite bacterial solution obtained in Example 1 and the chitosan solution at a volume ratio of 4:1, and mix them at -4°C for 24 hours to obtain a modified composite bacterial agent, which is the obtained biological algicide .

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Abstract

The invention relates to the technical field of water treatment, in particular to a biological algaecide as well as a preparation method and application thereof. The biological algaecide is obtained by fermenting and culturing a composite microbial agent and then modifying chitosan, wherein the composite microbial agent comprises actinomycetes, bacillus, alteromonas and myxobacteria. The preparation method of the biological algaecide comprises the following steps: (1) fermenting and culturing the composite microbial agent to obtain a composite bacterial solution; (2) mixing the composite bacterial solution obtained in the step (1) with a chitosan solution in proportion, and modifying the composite microbial agent by adopting the chitosan to obtain the biological algaecide. The biological algaecide is obtained by fermenting and culturing the composite microbial agent and then modifying the chitosan, has strong tolerance to environmental change in the actual using process, has the advantages of simplicity in operation, low cost and no secondary pollution when applied to algae removal of water bodies with algae bloom, and is high in algae removal efficiency and high in practicability.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a biological algicide and its preparation method and application. Background technique [0002] With the continuous acceleration of urbanization, water is enriched with a large number of nutrients (especially nitrogen and phosphorus), which continuously promotes the accelerated development of primary production and algae eutrophication. The eutrophication of water body is mainly caused by some phytoplankton's large-scale short-term explosive value-added or even high-density accumulation under the condition of sufficient nutrition, which causes the decrease of water quality transparency and the catastrophic aquatic ecological anomaly of normal life activities of aquatic organisms. Eutrophication is conducive to the periodic reproduction and dominant breeding environment of plankton and benthic organisms such as cyanobacteria. Microcystis aeruginosa blooms often occur in Ch...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/465C12R1/125C12R1/01
CPCC12N1/20C02F3/34
Inventor 杨秋婵刘静兰张保安
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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