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Fermentation method and device for producing gamma-polyglutamic acid through backflow type solid fermentation

A technology of polyglutamic acid and solid fermentation, which is applied in the biological field to achieve simple equipment, reduce the risk of contamination by bacteria, and improve efficiency

Active Publication Date: 2021-11-02
杨凌未来中科环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the defects and deficiencies in the prior art and solving the technical problems existing in the present stage of solid fermentation, the present invention provides a fermentation method and device for producing γ-polyglutamic acid by reflux solid fermentation

Method used

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  • Fermentation method and device for producing gamma-polyglutamic acid through backflow type solid fermentation
  • Fermentation method and device for producing gamma-polyglutamic acid through backflow type solid fermentation
  • Fermentation method and device for producing gamma-polyglutamic acid through backflow type solid fermentation

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Embodiment 1

[0042] This embodiment provides a kind of fermentation method that reflux solid fermentation produces gamma-polyglutamic acid, comprises the following steps:

[0043] Step 1: culturing Bacillus subtilis to obtain a seed culture; the Bacillus subtilis is Bacillus subtilis FEP205;

[0044] Step 2: mixing agricultural waste, industrial by-products and inorganic salts into a total material, adjusting the moisture content and pH of the total material, and using the adjusted material as a solid medium;

[0045] Step 3: inoculate the seed culture obtained in step 1 onto the solid medium described in step 2, keep it warm, pass sterile air into it for fermentation, and re-spray the leachate of the solid medium through a peristaltic pump after fermentation Pour it into the solid medium for reflux solid fermentation.

[0046] In this embodiment, step 1 includes inoculating the frozen Bacillus subtilis (Bacillus subtilis) FEP205 into the slant activation medium for activation, inoculatin...

Embodiment 2

[0074] The present embodiment carries out fermentation with the fermentation tank of 20L, comprises the following steps:

[0075] Step 1: including inoculating the frozen-preserved gamma-polyglutamic acid production strain into the slant activation medium for activation, inoculating the activated strain into the seed medium for expansion culture, and obtaining the seed culture.

[0076] Among them, the slope activation medium: peptone 10g / L, yeast powder 5g / L, NaCl 5g / L, agar 20g / L, pH 7.0, sterilized at 121°C for 30min, cooled, and prepared slope.

[0077] Seed medium: peptone 10g / L, yeast powder 5g / L, NaCl 5g / L, pH7.0, sterilized at 121°C for 30min;

[0078] Specifically, it includes inoculating the frozen Bacillus amyloliquefaciens FEP205 on the slant activation medium, culturing at 37°C for 24 hours for activation, picking up two rings of activated Bacillus amyloliquefaciens FEP205 from the inoculation loop, inoculating them in the seed medium, and inoculating at 37°C, Th...

Embodiment 3

[0088] This example is the same as Example 2, except that: 800 g / Kg of corn stalks (crushed to 1 cm) in the total material, 200 g / Kg of soybean meal, and no industrial by-products are added. In addition, in step 3, the ventilation rate is 2L / min, and the bottom leachate is sprayed every 4 hours. The main function of industrial by-products is to provide glutamic acid, which is used as a precursor for the synthesis of polyglutamic acid; due to the high protein content in soybean meal, it provides many different amino acids after degradation by bacteria, including glutamic acid, so it is also There may be no need to add other substances to provide the precursor.

[0089] The yield results of γ-polyglutamic acid show that the yield of γ-polyglutamic acid fermented by the fermentation method of this embodiment is 176.04g / Kg, and the output of traditional solid fermentation is 102.30g / Kg. Compared with the control group, this method The yield of γ-polyglutamic acid increased by 72....

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Abstract

The invention discloses a fermentation method and device for producing gamma-polyglutamic acid through reflux type solid fermentation. The fermentation method comprises the following steps: 1, culturing bacillus subtilis to obtain a seed culture; 2, mixing agricultural wastes, industrial byproducts and inorganic salts into a total material, adjusting the moisture content and pH of the total material, and using the adjusted material as a solid culture medium; 3, inoculating the seed culture obtained in the step 1 to the solid culture medium in the step 2, preserving heat, introducing sterile air for fermentation, circularly spraying the leachate of the solid culture medium into the solid culture medium again through a peristaltic pump after fermentation, and carrying out reflux type solid fermentation. Compared with traditional solid fermentation, the fermentation method has the advantages that the microbial activity is higher, moisture in solid materials is supplemented through backflow of percolate, full contact between microorganisms and substrates and moisture is enhanced, and the activity of growth and metabolism of the microorganisms is improved. And the final yield is increased by 150%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fermentation method and device for producing gamma-polyglutamic acid by reflux solid fermentation. Background technique [0002] γ-polyglutamic acid is a natural polymer compound, which is formed by L- or D-glutamic acid monomers linked by amide bonds between α-amino and γ-carboxyl groups. It has excellent water solubility, water absorption, and adsorption It has excellent properties such as non-toxicity, film-forming property, non-toxicity, edibility and biodegradability, and has been successfully applied in many fields such as agriculture, medicine, food, cosmetics and sewage treatment. Because of its wide application value, γ-polyglutamic acid has attracted much attention in recent years, and its demand is increasing year by year. At present, γ-polyglutamic acid is prepared by submerged fermentation of various bacillus, of which glucose, citric acid, L-glutamic acid...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12M1/16C12N1/20C12R1/125
CPCC12P13/02C12M21/16C12N1/20Y02W30/40
Inventor 王森林刘文吴彦武红帽
Owner 杨凌未来中科环保科技有限公司
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