Charcoal-loaded biological iron and manganese oxide material and preparation method and application thereof
A technology of manganese oxide and biochar, applied in the direction of chemical instruments and methods, applications, soil conditioning materials, etc., can solve the problems of high treatment cost and operating cost, damage to soil structure, low repair efficiency, etc., and achieve good oxidation and adsorption Action, huge surface area, effect of reducing scale
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[0044] The preparation process of the production medium is to firstly prepare the precursor of the production medium, then adjust the pH, then add manganese carbonate, and finally sterilize. Specifically, ammonium ferrous sulfate (0.15g / L) in the precursor of production medium; Yeast extract powder (0.075g / L); Sodium citrate (0.15g / L); Sodium pyrophosphate (0.05g / L) and Manganese carbonate (1g / L). Add manganese carbonate 0.2g / L.
[0045] In some examples of this embodiment, after the production and cultivation is completed, the precipitate obtained by centrifugation is the biochar-supported bio-iron-manganese oxide material.
[0046] In some examples of this embodiment, the strain is subjected to production culture after enrichment culture.
[0047] In one or more embodiments, the temperature of the enrichment culture is 15-35° C., and the time is 1-5 days.
[0048] In one or more embodiments, the composition of the enrichment medium for enrichment culture is 0.45-0.55 g / L ...
Embodiment approach
[0049] The third embodiment of the present invention provides an application of the biochar-loaded bio-iron-manganese oxide material in remediating arsenic-contaminated soil.
[0050] The fourth embodiment of the present invention provides a method for arsenic-contaminated soil. The biochar-loaded bio-iron-manganese oxide material is added to the arsenic-contaminated soil, and the moisture content of the arsenic-contaminated soil is adjusted, followed by cultivation and repair.
[0051] In some examples of this embodiment, the moisture content of the arsenic-contaminated soil is adjusted to 20-60%.
[0052] In some examples of this embodiment, the temperature of the culture restoration is 15-35° C., and the time is 2-6 weeks.
[0053] In order to enable those skilled in the art to understand the technical solution of the present invention more clearly, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.
Embodiment 1
[0055] 1) Enrichment culture of bacterial strains
[0056] Pseudomonas putida strain MnB1 was inoculated in Pseudomonas putida enrichment medium at a volume ratio of 2%, and then aerobic enrichment culture was carried out at 30°C under horizontal shaking at 180rpm for 3 days; The enrichment medium is composed of yeast extract powder 0.5g / L; acid hydrolyzed casein 0.5g / L; glucose 1g / L; calcium chloride dihydrate 0.29g / L; magnesium sulfate 0.82g / L; ferric chloride 1ml / L; trace element 1ml / L; PH=7; 1.8g agar / 100ml;
[0057] The selected bacterial strains were from the American Type Culture Collection (ATCC).
[0058] 2) Preparation of biochar-loaded bio-iron-manganese oxides:
[0059] Preparation medium: ferrous ammonium sulfate (0.15g / L); yeast extract powder (0.075g / L); sodium citrate (0.15g / L); sodium pyrophosphate (0.05g / L) and manganese carbonate (1g / L L), add 300mL medium into a 500mL Erlenmeyer flask, adjust pH=7. Add 0.2g / L manganese carbonate at the same time (add 0...
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