Specific lactic acid bacteria culture medium and culture method and application thereof

Abstract

The invention discloses a specific lactic acid bacteria culture medium and a culture method and application thereof. The culture medium is prepared by adding 8-12 g of tryptone, 10-15 g of beef extract, 8-12 g of yeast extract, 30-60 g of malt extract, 10-15 g of glucose, 1.5-2.5 g of diammonium hydrogen citrate, 1.5-2.5 g of dipotassium phosphate, 0.1-0.2 g of manganese sulfate monohydrate, 0.5-0.7 g of magnesium sulfate heptahydrate, 1-2 mL of Tween 80, 1-2 g of L-cysteine hydrochloride and 1-3 mg of vitamin B2 into per 1 L of tap water, and finally adding acetic acid to adjust to 4-6 g / 100mL. The culture medium formula and the culture method disclosed by the invention have the advantages of strong specificity, high culture efficiency, stable culture effect, simple components, low cost and easiness in commercialization, and the lactic acid bacteria HSLZ-75T and HSCY 2073 can be quickly and efficiently cultured.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a specific culture medium for lactic acid bacteria and its cultivation method and application. Background technique [0002] Studies have found that more than 90% of microorganisms without suitable culture methods still exist in nature, and only a small part of them can be cultured using traditional bacterial culture methods. The microorganisms cultivated by traditional methods are all known microorganisms, and there are few studies and reports on the medium components, isolation and cultivation methods of new microbial species. Difficulty in culturing microorganisms is related to special reasons that resist cultivation, including temperature, pH, oxygen, nutrition and many other factors. The cultivation of new microbial species has many technical challenges. [0003] Lactic acid bacteria generally refer to a large class of bacteria that can ferment sugars to produce a large amount ...

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Problems solved by technology

For example, the patent CN202010877453.8 discloses a lactic acid bacteria isolation medium formula and cultivation method, although the target lactic acid bacteria and the new species of lactic acid bacteria HSLZ-75 T The 16S rRNA sequence has 100% identity, but its target lactic acid bacteria is the lactic acid bacteria HSLZ-75 disclosed in the paper (Lactobacillus jinshani sp.nov., isolated from solid-state vinegar culture of Zhenjiangaromatic vinegar.Antonie van Leeuwenhoek) T Cannot grow on the medium formula of

Moreover, the target lactic acid bacteria in patent CN 202010877453.8 need to be cultivated on the solid plate for 7-14 days, and the cultivation time is relatively long; There are 9 kinds; it contains wine grains extract and is used in a large amount, which will easily lead to poor stability of the medium, and it is difficult for ordinary people to obtain wine grains extract, and the overall applicability is not strong

Patent CN 202011227512.3 discloses a culture medium formula and separation method for lactic acid bacteria Z-1, but its culture time is long, and it takes 7 days to form visible colonies on a solid plate; the medium composition is complex, and nearly 20 species need to be added in liquid culture Substance; complex operation, need to separately prepare a solution containing a large amount of complex vitamin B, before use, it needs to be sterilized through a membrane, and two kinds of acids, lactic acid and acetic acid, are needed to adjust the pH value; self-made bran extract is needed, and the stability of the medium is poor

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