Rooibos tea vinegar as well as preparation method and application thereof
A technology of rooibos tea and tea vinegar, applied in the preparation of vinegar, biochemical equipment and methods, methods based on microorganisms, etc., can solve the problems that the efficacy needs to be expanded, and achieve the purpose of inhibiting the accumulation and biological activity of fat in animals Definite, pure-scented results
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[0035] Preparation of yeast strains:
[0036] 1) Firstly, inoculate the yeast slant strain into Sabouraud culture medium, and cultivate it on a constant temperature shaker at 120 r / min at 28°C for 24 hours.
[0037] 2) Inoculate the liquid culture medium with a 4% inoculum size into a medium containing 10wt.% rooibos tea extract, 8wt.% white sugar and 3wt.% ammonium sulfate, and cultivate in a constant temperature shaker at 28°C and 120r / min After 24 hours, a grade I bacterial liquid was obtained.
[0038] 3) Inoculate the grade I bacteria liquid into a culture medium containing 20wt.% rooibos tea extract, 8wt.% white granulated sugar and 3wt.% ammonium sulfate according to the inoculum amount of 4%, and shake at a constant temperature of 120r / min at 28°C The bed was cultivated for 24 hours to obtain Grade II bacterial liquid.
[0039] 4) According to the above-mentioned medium formula, the content of rooibos tea extract is increased by 10wt.% each time, and other culture co...
Embodiment 1
[0045] The preparation method of the rooibos tea vinegar as the embodiment of the present invention comprises the following steps:
[0046] (1) Said that the original rooibos tea leaves were placed in a 500mL triangular flask, the volume of fermented water was 60%, the ratio of rooibos tea to water was 1:30 (g / L), and the addition accounted for 8% of the mass fraction of the liquid phase components white granulated sugar and 3% ammonium sulfate, adjust the pH to 6.0, sterilize at 121°C for 20 minutes, inoculate yeast with 3% inoculum after cooling, after mixing, place the triangular flask in a constant temperature shaker, and let it stand at 26°C for yeast 7 days of fermentation;
[0047] (2) After terminating yeast fermentation, inoculate the Acetobacterium seed solution with 8% inoculation amount, the acetic fermentation conditions include 120r / min, 20% dissolved oxygen, temperature 28°C, and the fermentation time is 10 days;
[0048] (3) After the fermentation in step (2),...
Embodiment 2
[0051] As the preparation method of rooibos tea vinegar according to the embodiment of the present invention, the only difference between this embodiment and Embodiment 1 is: adding 10% of white sugar and 3% of ammonium sulfate accounting for the mass fraction of the liquid phase components.
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