Polypeptide-nanogold modified glassy carbon electrode and application thereof

A technology of glassy carbon electrode and nano-gold, which is applied in the field of polypeptide-nano-gold modified glassy carbon electrode, to achieve the effects of improving electrochemical response signal, high synergy, good stability and reproducibility

Pending Publication Date: 2021-11-09
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the existing detection methods have their own limitations. In order to protect the health of consumers and meet the needs of import and export trade, it is necessary to develop a safe, easy to handle, easy to operate, fast results, and has a certain sensitivity and accuracy. The detection method of aflatoxin

Method used

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  • Polypeptide-nanogold modified glassy carbon electrode and application thereof
  • Polypeptide-nanogold modified glassy carbon electrode and application thereof
  • Polypeptide-nanogold modified glassy carbon electrode and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 prepares the glassy carbon electrode modified by polypeptide-nanogold, comprising the following steps:

[0039] Step 1. Soak all the glass instruments required for the synthesis of composite materials in an acid tank for one day, take them out and thoroughly rinse them with ultrapure water, and dry them in a constant temperature box at 37°C for later use.

[0040] Step 2, HAuCl 4 The solid was dissolved in ultrapure water to prepare a concentration of 10 mg·mL -1 mother solution, and then the freeze-dried polypeptide was dissolved in 20mL HEPES solution to prepare 0.04mmol·L -1 peptide solution.

[0041] Step 3, take 80 μL HAuCl 4 Add the mother liquor to 20mL of polypeptide HEPES solution, and stir at room temperature for 1-2h to obtain the PP-AuNPs composite material.

[0042] Step 4, the bare glassy carbon electrode was ground and polished on alumina powder, then ultrasonically treated in absolute ethanol and water, rinsed with water and placed in po...

Embodiment 2

[0043] The characterization of embodiment 2 modified electrode performance:

[0044] In order to verify whether the electrochemical immunosensor was successfully constructed, cyclic voltammetry was used to study and compare GCE, PP-AuNPs / GCE, antigen / PP-AuNPs / GCE, BSA / antigen / PP-AuNPs / GCE and test / BSA / antigen / The five electrodes of PP-AuNPs / GCE were in potassium ferricyanide solution (1mmol L -1 ) in the electrochemical response signal. Such as figure 1 As shown, these five modified electrodes all have a pair of good redox peaks in potassium ferricyanide solution. On GCE, the potential difference between the redox peaks is less than 75mV, indicating that the electrode activation is better and the conductivity is better. The curve of the electrode modified by PP-AuNPs is more beautiful, and the oxidation peak is slightly reduced, the reduction peak is more obvious, and the peak potential difference is reduced, which shows that PP-AuNPs has been successfully modified on GCE,...

Embodiment 3

[0046] The optimization of embodiment 3 experimental parameters affects the detection of aflatoxin:

[0047] Optimization of antigen concentration ( image 3): Detection of electrochemical signals by differential pulse voltammetry (potential range: -0.2-0.6V, amplitude: 0.05V). Under the conditions of room temperature (25°C), pH=7.4, and incubation time of 20 minutes, set the concentration ratio of antigen and antibody to 2:1, and the detection concentration is 5.0ng·mL -1 The mixed test solution of aflatoxin and antibody, the electrochemical response intensity of aflatoxin is affected by the concentration of antigen and antibody, resulting in different response signals. At the same time, it is compared with the blank liquid to be tested, and the current to be tested - blank current = ΔI is defined, and the detection effect is judged by the size of ΔI. When the concentration of Aspergillus flavus antigen was 10.0 μg·mL -1 and 1.0 μg·mL -1 When , the reduction peak current ...

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Abstract

The invention belongs to the technical field of detection of the total amount of aflatoxin in peanuts and products thereof in food, and discloses a polypeptide-nanogold (PP-AuNPs) modified glassy carbon electrode and a method for detecting the total amount of aflatoxin in peanuts and products thereof by using the electrode. The modified material is prepared by the following steps: (1) adding a HAuCl4 mother solution into a polypeptide-containing HEPES buffer solution, and performing stirring at room temperature to obtain a PP-AuNPs composite material; (2) grinding and polishing the bare glassy carbon electrode on alumina powder, then sequentially carrying out ultrasonic treatment in absolute ethyl alcohol and ultrapure water, then performing washing with water, and placing thebare glassy carbon electrode in a potassium ferricyanide solution for cyclic voltammetry scanning; and (3) dispensing the PP-AuNPs composite material obtained in the step (1) on the surface of the bare glassy carbon electrode treated in the step (2), and performing drying at constant temperature to obtain the PP-AuNPs modified glassy carbon electrode (PP-AuNPs / GCE for short). The modification material is dispensed on the surface of an activated bare glassy carbon electrode and dried, an antigen, a confining liquid (BSA) and a to-be-detected sample are sequentially dispensed, incubation is performed, and then electrochemical signal detection is performed. According to the detection method, the response signal of the total amount of aflatoxin can be improved, the reliability of aflatoxin detection is improved, and qualitative or quantitative detection of the residual amount of aflatoxin in peanuts and products thereof is realized.

Description

technical field [0001] The invention relates to the technical field of residual detection of the total amount of aflatoxin in peanuts and its products, in particular to a glassy carbon electrode modified by polypeptide-nano gold (PP-AuNPs) and using the electrode to construct an electrochemical immunosensor to detect aflatoxin total method. Background technique [0002] Aflatoxins are secondary metabolites of toxin-producing strains such as Aspergillus flavus and Aspergillus parasitica, and are also recognized as the most carcinogenic natural substances, mainly including aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ), aflatoxin M 1 (AFM 1 ) and aflatoxin M 2 (AFM 2 ) and more than 20 kinds, among which peanuts are mainly the first 4 kinds. The physical and chemical properties of aflatoxins are particularly stable. After being digested and absorbed by humans and animals, aflatoxins in food cannot be decomposed and ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 毛烨炫张西亚党梦黄现青谢新华宋莲军张平安王田林杨缓丁明月王尤一
Owner HENAN AGRICULTURAL UNIVERSITY
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